[논문]제주도 야생버섯 hyphosphere 토양에서 분리된 국내 미기록 진균 11종 보고

노형진; 김예인; 이동형; 고평열; 박혜성; 이강효; 김성환

doi:10.14480/jm.2023.21.4.228

제주도 야생버섯 hyphosphere 토양에서 분리된 국내 미기록 진균 11종 보고
Eleven previously unrecorded fungal species isolated from hyphosphere soil supporting wild mushrooms in Jeju Island 원문보기

Journal of mushrooms = 한국버섯학회지, v.21 no.4, 2023년, pp.228 - 240

노형진 (단국대학교 생명과학부 미생물학전공) , 김예인 (단국대학교 생명과학부 미생물학전공) , 이동형 (단국대학교 생명과학부 미생물학전공) , 고평열 (제주대학교 친환경농업연구소) , 박혜성 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) , 이강효 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) , 김성환 (단국대학교 생명과학부 미생물학전공)

초록
AI-Helper

야생버섯의 결실에 미치는 미생물의 영향을 파악하기 위하여 제주도 곶자왈 지역에서 발견된 야생버섯, 끈적버섯 (Cortinarius violaceus), 달걀버섯 (Amanita hemibapha), 색시졸각버섯 (Laccaria vinacelavellanea), 흰알광대버섯 (Amanita verna)의 근권토양 균류를 조사하였다. 형태학적 특성 조사와 ITS, LSU rDNA, β-tubulin 유전자 서열에 대한 분자생물학적 분석을 바탕으로 동정하는 과정에서 국내 미기록 진균 11종을 분리하였다. 이들 진균은 Arthrinium kogelbergensis, Kalmusia longisporum, Keithomyces carneum, Neopyrenochaeta cercidis, Penicillium ranomafanaense, Phomatodes nebulosa, Pyrenochaeta nobilis, Tollypocladium album, Talaromyces kendrickii, Talaromyces qii, Umbelopsis gibberispora이다. 이들 11종에 진균에 대한 형태적 특성과 계통발생학적 위치를 기술하였다.

Abstract ▼ AI-Helper

To understand microorganism effects on wild mushroom fruiting bodies, we investigated the fungi in hyphosphere soil supporting wild mushroom species Cortinarius violaceus, Amanita hemibapha, Laccaria vinacelavellanea, and Amanita verna found in the Gotjawal area of Jeju Island. Fungal species identification based on morphological traits and molecular analysis of ITS, LSU rDNA, and β-tubulin gene sequences resulted in isolation and identification of eleven fungal species previously unrecorded in Korea. These newly-recorded species are: Arthrinium kogelbergensis, Kalmusia longisporum, Keithomyces carneum, Neopyrenochaeta cercidis, Penicillium ranomafanaense, Phomatodes nebulosa, Pyrenochaeta nobilis, Tolypocladium album, Talaromyces kendrickii, Talaromyces qii, and Umbelopsis gibberispora, and their morphological characteristics and phylogenetic positions are described.

주제어

표/그림 (11)

그림 Fig 1. Colony morphology on PDA (A-F, M-Q), light microscopic images (G-J, L-W) and SEM image (K) of the 11 unrecorded fungal species isolated from hyphosphere of wild mushrooms in Jeju Island in 2019. A, G: Apiospora kogelbergensis DK14-10. B, H: Kalmusia longisporum DK14-12. C, I: Keithomyces carneum DK14-31. D, J: Neopyrenochaeta cercidis DK14-13. E, K: Penicillium ranomafanaense DK14-30. F, L: Phomatodes nebulosa DK14-15. M, S: Pyrenochaeta nobilis DK14-16. N, T: Tolypocladium album DK14-20. O, U: Talaromyces kendrickii DK14-18. P, V: Talaromyces qii DK14-19. Q, W: Umbelopsis gibberispora DUCC17752. Scale bar = 10 μm).
그림 Fig 2. Neighbor joining phylogenetic tree based on the concatenated ITS region and LSU rDNA nucleotide sequences of Apiospora kogelbergensis DK14-10(NIBRFGC000505467). The number of nodes represents the reliability value through 1000 bootstrap repetitions, and those below 60% were removed. Fungal strain isolated in this study is indicated in bold.
그림 Fig 3. Neighbor joining phylogenetic tree based on the concatenated ITS region and LSU rDNA nucleotide sequences of Kalmusia longispora DK14-12(NIBRFGC000505477). The number of nodes represents the reliability value through 1000 bootstrap repetitions, and those below 60% were removed. Fungal strain isolated in this study is indicated in bold.
그림 Fig 4. Neighbor joining phylogenetic tree based on the concatenated ITS region and LSU rDNA nucleotide sequences of Keithomyces carneus DK14-31(NIBRFGC000505475). The number of nodes represents the reliability value through 1000 bootstrap repetitions, and those below 60% were removed. Fungal strain isolated in this study is indicated in bold.
그림 Fig 5. Neighbor joining phylogenetic tree based on the concatenated ITS region and LSU rDNA nucleotide sequences of Neopyrenochaeta cercidis DK14-13(NIBRFGC000505479). The number of nodes represents the reliability value through 1000 bootstrap repetitions, and those below 60% were removed. Fungal strain isolated in this study is indicated in bold.
그림 Fig 6. Neighbor joining phylogenetic tree based on the concatenated ITS region and BenA gene nucleotide sequences of Penicillium ranomafanaense DK14-30(NIBRFGC000505470). The number of nodes represents the reliability value through 1000 bootstrap repetitions, and those below 60% were removed. Fungal strain isolated in this study is indicated in bold.
그림 Fig 7. Neighbor joining phylogenetic tree based on the concatenated ITS region and BenA gene nucleotide sequences of Phomatodes nebulosa DK14-15 (NIBRFGC000505476). The number of nodes represents the reliability value through 1000 bootstrap repetitions, and those below 60% were removed. Fungal strain isolated in this study is indicated in bold.
그림 Fig 8. Neighbor joining phylogenetic tree based on the concatenated ITS region and LSU rDNA nucleotide sequences of Pyrenochaeta nobilis DK14-16(NIBRFGC000505671). The number of nodes represents the reliability value through 1000 bootstrap repetitions, and those below 60% were removed. Fungal strain isolated in this study is indicated in bold.
그림 Fig 9. Neighbor joining phylogenetic tree based on the concatenated ITS region and LSU rDNA nucleotide sequences of Tolypocladium album DK14-20 (NIBRFGC000505478). The number of nodes represents the reliability value through 1000 bootstrap repetitions, and those below 60% were removed. Fungal strain isolated in this study is indicated in bold.
그림 Fig 10. Neighbor joining phylogenetic tree based on the concatenated ITS region and BenA gene nucleotide sequence of Talaromyces qii DK14-19(NIBRFGC000505483) and Talaromyces kendrickii DK14-18(NIBRFGC000505482). The number of nodes represents the reliability value through 1000 bootstrap repetitions, and those below 60% were removed. Fungal strain isolated in this study is indicated in bold.
그림 Fig 11. Neighbor joining phylogenetic tree based on the concatenated ITS region and LSU rDNA nucleotide sequences of Umbelopsis angularis DUCC17752 (NIBRFGC000508333). The number of nodes represents the reliability value through 1000 bootstrap repetitions, and those below 60% were removed. Fungal strain isolated in this study is indicated in bold.

참고문헌 (45)

Assemie A, Abaya G. 2022. The effect of edible mushroom？on health and their biochemistry. Int J Microbiol 2022: 8744788.
Cerar D, Malallah YM, Howard SJ, Bowyer P, Denning DW. 2009. Isolation, identification and susceptibility of？Pyrenochaeta romeroi in a case of eumycetoma of the？foot in the UK. Int J Antimicrob Agents 34(6): 617-618.
Chen Q, Jiang JR, Zhang GZ, Cai L, Crous PW. 2015.？Resolving the Phoma enigma. Stud Mycol 82: 137-217.
Choi MA, Park SJ, Ahn GR, Kim SH. 2014. Identification？and characterization of Paraconiothyrium brasiliense from？garden plant Pachysandra terminalis. Kor J Mycol 42(4):？262-268.
Crous PW, Groenewald JZ. 2013. A phylogenetic reevaluation of Arthrinium. IMA Fungus 4(1): 133-154.

상세보기
Gams W. 1980. Chaunopycnis alba gen. et sp. nov., a soil？fungus intermediate between the Moniliales and？Sphaeropsidales. Pers: Mol Phylogeny Evol Fungi 11(1):？75-79.
Houbraken J, Visagie CM, Meijer M, Frisvad JC, Busby？PE, Pitt JI, Seifert KA, Louis-Seize G, Demirel R, Yilmaz？N, Jacobs K, Christensen M, Samson RA. 2014. A？taxonomic and phylogenetic revision of Penicillium？section Aspergilloides. Stud Mycol 78: 373-451.
Jang YC, Lee CW. 2009. Gotjawal Forest in Jeju Island as？an internationally important wetland. J Wetlands Res 11:？99-104.
Jayasiri SC, Hyde KD, Jones EBG, McKenzie EHC, Jeewon？R, Phillips AJL, Bhat DJ, Wanasinghe DN, Liu JK, Lu？YZ, Kang JC, Xu J, Karunarathna SC. 2019. Diversity,？morphology and molecular phylogeny of Dothideomycetes？on decaying wild seed pods and fruits. Mycosphere 10(1):？1-186.

상세보기
Kang JC, Kim MS. 2012. Seasonal variation in depth-stratified macroalgal assemblage patterns on Marado, Jeju？Island, Korea. Algae 27(4): 269-281.

원문보기 상세보기
Kepler RM, Humber RA, Bischoff JF, Rehner SA. 2014.？Clarification of generic and species boundaries for？Keithomyces and related fungi through multigene？phylogenetics. Mycologia 106(4): 811-829.
Karavani A, De Caceres M, de Aragon JM, Bonet JA, de-Miguel S. 2018. Effect of climatic and soil moisture？conditions on mushroom productivity and related？ecosystem services in Mediterranean pine stands facing？climate change. Agric For Meteorol 248: 432-440.

상세보기
Kauserud H, Stige LC, Vik JO, Okland RH, Hoiland K,？Stenseth NC. 2008. Mushroom fruiting and climate？change. Proc Natl Acad Sci U S A 105(10): 3811-3814.

상세보기
Kim CS. 2009. Vascular plant diversity of Jeju Island,？Korea. Korean J Plant Res 22(6): 558-570.

원문보기 상세보기
Kim JT, Park IH, Ryu KY, Cheon JU, Yu SH. 2003. Corky？root of tomato caused by Pyrenochaeta lycopersici in？Korea. Plant Pathol J 19(3): 181-183.

원문보기 상세보기
Ko PY. 2013. Study on species diversity of indigenous？mushrooms in Jeju [dissertation]. Jeju: Jeju National？University.
Ko PY, Seok SJ, Lee HB, Ko HS, Jeun YC. 2014. Species？diversity of spontaneous mushrooms on Jeju Island. Kor J？of Mycol 42(2): 104-132.
Kumar S, Stecher G, Li M, Knyaz C, Tamura K. 2018.？MEGA X: Molecular Evolutionary Genetics Analysis？across computing platforms. Mol Bio Evol 35(6): 1547-1549
Lee SH, Ko PY, Jeun YC. 2018. Domestic unrecorded？species found in the eastern part of Jeju Island. Kor J？Mycol 46(2): 105-113.
Li H, Tian Y, Menolli Jr N, Ye L, Karunarathna SC, Perez-Moreno J, Rahman MM, Rashid MH, Phensintham P,？Rizal L, Kasuya T, Lim YW, Dutta AK, Khalid AN,？Huyen LT, Balolong MP, Baruah G, Madawala S,？Thongklang N, Hyde KD, Kirk PM, Xu J, Sheng J, Boa？E, Mortimer PE. 2021. Reviewing the world's edible？mushroom species: A new evidence-based classification？system. Compr Rev Food Sci Food Saf 20(2): 1982-2014.
Llanaj X, Toros G, Hajdu P, Abdalla N, El-Ramady H, Kiss？A, Solberg SO, Prokisch J. 2023. Biotechnological？applications of mushrooms under the water-energy-food？nexus: crucial aspects and prospects from farm to？pharmacy. Foods 12(14): 2671
National Institute of Biological Resources. 2020. Survey and？community analysis of indigenous fungi related to the？growth of wild animals and plants (II). ISBN 11-1480592-001747-01.
Nei M and Kumar S. 2000. Molecular Evolution and？Phylogenetics. Oxford University Press, New York.
Oh S-Y, Fong JJ, Park MS, Lim YW. 2016. Distinctive？feature of microbial communities and bacterial functional？profiles in Tricholoma matsutake dominant soil. PLoS？ONE 11(12): e0168573.

상세보기
Oh SY, Yang JH, Woo JJ, Oh SO, Hur JS. 2020. Diversity？and distribution patterns of endolichenic fungi in Jeju？Island, South Korea. Sustainability 12(9): 3769.
Phan CW, Sabaratnam V. 2012. Potential uses of spent？mushroom substrate and its associated lignocellulosic？enzymes. Appl Microbiol Biotechnol 96(4): 863-873.
Pitt JI. 1991. Penicillium toxins. In B.R. Champ, E. Highley,？A.D. Hocking, and J.I. Pitt. (ed.), Fungi and Mycotoxins？in Stored Products: Proceedings of an international？conference held at Bangkok, Thailand, 23-26 April 1991,？ACIAR Proc.
Quandt CA, Kepler RM, Gams W, Araujo JP, Ban S, Evans？HC, Hughes D, Humber R, Hywel-Jones N, Li Z,？Luangsa-ard J, Rehner SA, Sanjuan T, Sato H, Shrestha？B, Sung GH, Yao YJ, Zare R, Spatafora JW. 2014.？Phylogenetic-based nomenclatural proposals for？Ophiocordycipitaceae (Hypocreales) with new combinations？in Tolypocladium. IMA Fungus 5(1): 121-134.

상세보기
Raaijmakers JM, Paulitz TC, Steinberg C, Alabouvette C,？Moenne-Loccoz Y. 2009. The rhizosphere: a playground？and battlefield for soilborne pathogens and beneficial？microorganisms. Plant Soil 321: 341-361.
Raper KB, Alexander DF, Coghill RD. 1944. Penicillin: II.？Natural variation and penicillin production in Penicillium？notatum and allied species 1, 2. J Bacteriol 48(6): 639-659.
Royse DJ, Baars J, Tan Q. 2017. Current overview of？mushroom production in the world. In C.Z. Diego, A.？Pardo-Gimenez. (e.d.), Edible and Medicinal Mushrooms:？Technology and Applications, John Wiley & Sons Ltd.
Saitou N, Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Bio？Evol 4(4): 406-425.
Sanchez-Garcia M, Ryberg M, Khan FK, Varga T, Nagy LG,？Hibbett DS. 2020. Fruiting body form, not nutritional？mode, is the major driver of diversification in mushroom-forming fungi. Proc Natl Acad Sci U S A 117(51): 32528-32534.

상세보기
Sangthong S, Pintathong P, Pongsua P, Jirarat A, Chaiwut P. 2022. Polysaccharides from Volvariella volvacea？mushroom: Extraction, biological activities and cosmetic？efficacy. J Fungi (Basel) 8(6): 572.
Schneider R. 1979. The genus Pyrenochaeta De Notaris.？Mitteilungen aus der Biologischen Bundesanstalt fuer？Land und Forstwirtschaft. In German.
Smith JJ, Lilly MD, Fox RI. 1990. The effect of agitation？on the morphology and penicillin production of？Penicillium chrysogenum. Biotechnol Bioeng 35(10): 1011-1023.

상세보기
Tang LQ, Hyun MW, Yun YH, Sun DY, Kim SH, Sung？GH. 2012. New record of Mariannaea elegans var.？elegans in Korea. Mycobiology 40(1): 14-19.

원문보기 상세보기
Teponno RB, Noumeur S., Helaly SE, Huttel S, Harzallah？D, Stadler M. 2017. Furanones and anthranilic acid？derivatives from the endophytic fungus Kalmusia？variisporum. Molecules 22(10): 1674.
Verkley GJM, Dukik K, Renfurm R, Goker M, Stielow JB. 2014. Novel genera and species of coniothyrium-like？fungi in Montagnulaceae(Ascomycota). Persoonia 32: 25-51.

상세보기
Visagie CM, Yilmaz N, Frisvad JC, Houbraken J, Seifert？KA, Samson RA, Jacobs K. 2015. Five new Talaromyces？species with ampulliform-like phialides and globose rough？walled conidia resembling T. verruculosus. Mycoscience？56(5): 486-502.

상세보기
Wang QM, Zhang YH, Wang B, Wang L. 2016.？Talaromyces neofusisporus and T. qii, two new species of？section Talaromyces isolated from plant leaves in Tibet,？China. Sci Rep 6: 18622.

상세보기
White TJ, Bruns TD, Lee SB, Taylor JW. Amplification and？direct sequencing of fungal ribosomal RNA genes for？phylogenetics. In Innis MA, Gelfand D & Sninsky JJ.？(ed.), PCR Protocols: A Guide to Methods and？Applications, Academic Press, USA.
Yang X, Feng P, Yin Y, Bushley K, Spatafora JW, Wang C. 2018. Cyclosporine biosynthesis in Tolypocladium？inflatum benefits fungal adaptation to the environment.？mBio 9(5): e01211-18.

상세보기
Yin C, Luo F, Zhang H, Fang X, Zhu T, Li S. 2021. First？report of Apiospora kogelbergensis causing blight disease？of Bambusa intermedia in Sichuan Province, China. Plant？Dis 105(1): 214.
Yun YH, Hyun MW, Suh DY, Kim YM, Kim SH. 2009.？Identification and characterization of Eurotium rubrum？isolated from Meju, Korea. Mycobiology 37(4):251-257.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증