[논문]정금나무 열매(Fruit of Vaccinum oldhami)의 분리 정제물(클로로겐산, 퀘르세틴 및 퀘르시트린)에 관한 항산화 및 미백활성 검증

채정우; 오민정; 염현지; 이진영

doi:10.5352/jls.2023.33.2.115

정금나무 열매(Fruit of Vaccinum oldhami)의 분리 정제물(클로로겐산, 퀘르세틴 및 퀘르시트린)에 관한 항산화 및 미백활성 검증
Antioxidant and Whitening Activities of Chlorogenic Acid, Quercetin, and Quercitrin from the Fruit of Vaccinum oldhami 원문보기

생명과학회지 = Journal of life science, v.33 no.2, 2023년, pp.115 - 128

채정우 (경기도산림환경연구소) , 오민정 (호서대학교 화장품생명공학부) , 염현지 (호서대학교 화장품생명공학부) , 이진영 (호서대학교 화장품생명공학부)

초록
AI-Helper

정금나무 열매(Fruit of Vaccinum oldhami)를 분리 정제하여 클로로겐산(CA), 퀘르세틴(QT) 및 퀘르시트린(QR)을 얻었다. 전자공여능 측정 결과, CA, QT, QR은 각각 1,000 ㎍/ml의 농도에서 91.9%, 89.9%, 77.4%의 효과를 나타내었다. ABTS⁺ 라디칼 소거능 측정 결과, QT 및 QR은 1,000 ㎍/ml의 농도에서 각각 99.5%, 91.4%를 나타내었으며, CA는 100 ㎍/ml의 농도에서 90% 이상의 소거능을 나타내었다. Tyrosinase 억제 활성 측정 결과, CA, QT, QR은 각각 1,000 ㎍/ml이 농도에서 29.5%, 34.7%, 23.7%의 저해율을 나타내었다. MTT assay를 통해 B16F10의 세포 생존율을 측정한 결과, CA, QT, QR 모두 100 ㎍/ml의 농도에서 80% 이상의 생존율을 나타내었다. 단백질 발현을 측정한 결과, 농도가 증가함에 따라 CA에서 TRP-1, TRP-2의 단백질 발현이 억제되는 것을 확인할 수 있었으며 QT에서는 TRP-1, TRP-2 및 tyrosinase에서 우수한 단백질 발현 억제율을 나타내었다. CA의 mRNA 발현 억제율을 측정한 결과, MITF의 mRNA 발현 억제 효과가 우수함을 확인하였으며, TRP-1, TRP-2 및 tyrosinase의 mRNA 발현량은 농도가 증가할수록 감소하였다. QT는 농도가 증가할수록 MITF, TRP-2, tyrosinase의 mRNA 발현이 감소하였다. QR의 mRNA 발현 억제율을 측정한 결과, 농도가 증가할수록 mRNA 발현량이 감소하는 것을 확인할 수 있었다. 이하의 결과에 따라 CA, QT, QR의 항산화 및 미백 효과를 확인할 수 있었다.

Abstract ▼ AI-Helper

The fruit of Vaccinum oldhami was separated and purified to obtain the compounds chlorogenic acid (CA), quercetin (QT), and quercitrin (QR). The electron-donating abilities of CA, QT, and QR at 1,000 ㎍/ml were 91.9%, 89.9%, and 77.4%, respectively QT and QR showed 99.5% and 91.4% ABTS+ radical scavenging ability at a 1,000 ㎍/ml concentration, respectively, and CA showed a 95% ability or higher at 100 ㎍/ml. Regarding tyrosinase inhibitory activity, CA, QT, and QR exhibited 29.5%, 34.7%, and 23.7% efficacy, respectively, at 1,000 ㎍/ml. Regarding the cell viability for melanoma cells (B16F10) assessed through MTT assay, CA, QT, and QR showed cell a viability of 80% or more at 100 ㎍/ml. To measure the deterrent of protein expression, CA affected TRP-1 and TRP-2 in accordance with increases in concentration. The protein expression inhibition rate of QT was excellent for TRP-1, TRP-2, and tyrosinase. CA was confirmed to have an excellent mRNA expression inhibitory effect against MITF, and the amount of mRNA expression of TRP-1, TRP-2, and tyrosinase decreased with an increase in the CA concentration. As the concentration of QT increased, the mRNA expression of MITF, TRP-2, and tyrosinase decreased. QR decreased the amount of mRNA as the QR concentration increased. The excellent antioxidant and whitening effects of CA, QT, and QR were thus confirmed.

주제어

표/그림 (24)

그림 Fig. 1. The procedure for extraction from Fruit of Vaccinum oldhami.
그림 Fig. 2. Separation flow diagram of Fruit of Vaccinum oldhami Extracts.
그림 Fig. 3. Chemical structure of compound 1-3. Compound 1 - ¹H-NMR (600 MHz, MeOD) δ : 7.07 (1H, brs, H-2'), 6.96 (1H, brd, J = 6.6 Hz, H-5'), 6.80 (1H, dd, J = 2.4, 7.2 Hz, H-6'), 7.59 (1H, d, J = 15.6 Hz, H-7'), 6.31 (1H, d, J = 15.6 Hz, H-8'), 2.10 – 2.21 (4H, m, H-2, 6), 3.73 (1H, brs, H-3), 4.19(1H, brs, H-4), 5.39(1H, s, H-5). ¹³C-NMR (150MHz, MeOD) δ : 167.7 (COO), 148.1 (C-4'), 145.4 (C-3'), 145.3 (C-7') 126.4 (C-1'), 121.5 (C-6'), 115.1 (C-2') 114.1 (C-5'), 113.7 (C-8'), 73.5 (C-1), 71.5 (C-3), 71.2 (C-4), 39.0 (C-2), 37.5 (C-6). Compound 2 - ¹H-NMR (600 MHz, MeOD) δ : 7.33 (1H, brs, H-2'), 7.32 (1H, dd, J = 2.4, 8.4, H-6'), 6.96 (1H, d, J = 8.4, H-5'), 6.40 (1H, d, J = 1.8, H-8), 6.23 (1H, d, J = 1.8, H-6), 5.36 (1H, s, anomeric), 4.20 (1H, s, H-2''), 3.87 (1H, m, H-4''), 3.65 (1H, m, H-3''), 3.07 (1H, t, J = 11.4, H-5'', 1.05, 3H, s, rha-CH3), ¹³C-NMR (150 MHz, MeOD) δ : 178.3 (C=O), 164.5 (C-7), 161.8 (C-5), 157.8 (C-2), 157.1 (C-9), 148.4 (C-4'), 145.1 (C-3'), 135.3 (C-3), 121.4 (C-1'), 121.0 (C-6'), 115.4 (C-2'), 115.1 (C-5'), 104.4 (C-10), 101.8 (C-1''), 98.5 (C-6), 93.4 (C-8), 72.3 (C-4''), 70.5 (C-2''), 70.4 (C-3''), 69.5 (C-4''), 16.2 (C-6''). Compound 3 - ¹H-NMR (600 MHz, MeOD) δ : 7.75 (1H, brs, H-2'), 7.66 (1H, dd, J = 2.4, 8.4, H-6'), 6.91 (1H, d, J = 8.4, H-5'), 6.40 (1H, d, J = 1.8, H-8), 6.20 (1H, d, J = 1.8, H-6) ¹³C-NMR (150 MHz, MeOD) δ : 175.9 (C=O), 164.1 (C-7), 161.1 (C-5), 156.8 (C-9), 147.3 (C-4'), 146.6 (C-2), 144.8 (C-3'), 135.8 (C-3), 122.7 (C-1'), 120.2 (C-6'), 114.8 (C-5'), 114.5 (C-2'), 103.1 (C-10), 97.8 (C-6), 93.0 (C-8).
그림 Fig. 4. Linearity review results of compound 1-3.
$Fig. 5. Chromatogram of ethyl acetate fractions from Fruit of Vaccinum oldhami.$ 그림 Fig. 5. Chromatogram of ethyl acetate fractions from Fruit of Vaccinum oldhami.
표 Table 1. Sequence of the primers used for RT-PCR
그림 Fig. 6. Electron donating ability of Fruit of Vaccinum oldhami separation and purification chlorogenic acid.
그림 Fig. 7. Electron donating ability of Fruit of Vaccinum oldhami separation and purification quercetin.
그림 Fig. 8. Electron donating ability of Fruit of Vaccinum oldhami separation and purification quercitrin.
그림 Fig. 9. ABTS⁺ radical scavenging ability of Fruit of Vaccinum oldhami separation and purification chlorogenic acid.
그림 Fig. 10. ABTS⁺ radical scavenging ability of Fruit of Vaccinum oldhami separation and purification quercetin.
그림 Fig. 11. ABTS⁺ radical scavenging ability of Fruit of Vaccinum oldhami separation and purification quercitrin.
그림 Fig. 12. Inhibition rate of Fruit of Vaccinum oldhami separation and purification chlorogenic acid on tyrosinase.
그림 Fig. 13. Inhibition rate of Fruit of Vaccinum oldhami separation and purification quercetin on tyrosinase.
그림 Fig. 14. Inhibition rate of Fruit of Vaccinum oldhami separation and purification quercitrin on tyrosinase.
그림 Fig. 15. Cell viability of extracts from Fruit of Vaccinum oldhami separation and purification chlorogenic acid on melanoma cell (B16f10).
그림 Fig. 16. Cell viability of extracts from Fruit of Vaccinum oldhami separation and purification quercetin on melanoma cell (B16f10).
그림 Fig. 17. Cell viability of extracts from Fruit of Vaccinum oldhami separation and purification quercitrin on melanoma cell (B16f10).
그림 Fig. 18. MITF, TRP-1, TRP-2 and tyrosinase protein expression rate of Fruit of Vaccinum oldhami separation and purification chlorogenic acid on melanoma cell (B16f10).
그림 Fig. 19. MITF, TRP-1, TRP-2 and tyrosinase protein expression rate of Fruit of Vaccinum oldhami separation and purification quercetin on melanoma cell (B16f10).
그림 Fig. 20. MITF, TRP-1, TRP-2 and tyrosinase protein expression rate of Fruit of Vaccinum oldhami separation and purification quercitrin on melanoma cell (B16f10).
그림 Fig. 21. MITF, TRP-1, TRP-2 and tyrosinase mRNA expression rate of Fruit of Vaccinum oldhami separation and purification chlorogenic acid on melanoma cell (B16f10).
그림 Fig. 22. MITF, TRP-1, TRP-2 and tyrosinase mRNA expression rate of Fruit of Vaccinum oldhami separation and purification quercetin on melanoma cell (B16f10).
그림 Fig. 23. MITF, TRP-1, TRP-2 and tyrosinase mRNA expression rate of Fruit of Vaccinum oldhami separation and purification quercitrin on melanoma cell (B16f10).

AI 본문요약
AI-Helper

문제 정의

Even though research is currently being conducted on Fruit of Vaccinum oldhami, there is still little research on Fruit of Vaccinum oldhami. Therefore, the aim of the present study was to investigate the antioxidant effects and whitening activities of Fruit of Vaccinum oldhami by examining the effects of purified isolates of chlorogenic acid (CA), quercetin (QT), and quercitrin (QR) on B16F10 cells.

참고문헌 (27)

Blois, M. S. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181, 1199-1120.？

상세보기
Carmichael, J., DeGraff, W. G., Gazdar, A. F., Minna, J. D. and Mitchell, J. B. 1987. Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47, 936-942.？

상세보기
Cha, J. Y., Yang, H. J., Jeong, J. J., Seo, W. S., Park, J. S., Ok, M. and Cho, Y. S. 2010. Tyrosinase inhibition activity and antioxidant capacity by fermented products of some medicinal plants. J. Life Sci. 20, 940-947.？

원문보기 상세보기
Chung, D. M., Chung, Y. C. and Chun, H. K. 2011. Spectrophotometric assay for determination of chlorogenic acid using green pigment formation and quantitative analysis of chlorogenic acid in Blueberry Leaf. J. Life Sci. 21, 610-612.？

원문보기 상세보기
Hearing, V. J. 1999. Biochemical control of melanogenesis and melanosomal organization. J. Invest. Dermatol. Symp. Proc. 4, 24-28.？

상세보기
Hirotoshi, T., Hisato, K., Ryoko, K. T., Kazuo, N., Masao, Y., Haruki, K. and Chizuko, Y. 2013. Antioxidant activities and anti-cancer cell proliferation properties of Natsuhaze (Vaccinium oldhamii Miq.), Shashanbo (V. bracteatum Thunb.) and Blueberry Cultivars. Plants 2, 57-71.？

상세보기
Hwang, C. E., Kim, S. C., Cho, C. S., Song, W. Y., Joo, O. S. and Cho, K. M. 2020. Comparison of chlorogenic acid and rutin contents and antioxidant activity of Dendropanax morbiferus extracts according to ethanol concentration. KoSFoP. 27, 880-887.？
Hwang, J. H. and Lee, B. M. 2007. Inhibitory effects of plant extracts on tyrosinase, L-DOPA oxidation, and melanin synthesis. J. Toxicol. Environ. Health Part A 70, 393-407.？

상세보기
Imokawa, G. and Mishima, Y. 1981. Biochemical characterization of tyrosinase inhibitors using tyrosinase binding affinity chromatography. Br. J. Dermatol. 104, 531-539.？

상세보기
Iwata, M., Corn, T., Iwata, S., Everett, M. A. and Fuller, B. B. 1990. The relationship between tyrosinase activity and skin color in human foreskins. J. Invest. Dermatol. 95, 9-15.？

상세보기
Kameyama, K., Sakai, C., Kuge, S., Nishiyama, S., Tomita, Y., Ito, S., Wakamatsu, K. and Hearing, V. J. 1995. The expression of tyrosinase, tyrosinase-related proteins 1 and 2 (TRP1 and TRP2), the silver protein, and a melanogenic inhibitor in human melanoma cells of differing melanogenic activities. Pigment. Cell. Res. 8, 97-104.？

상세보기
Kim, J. W., Kim, H. I., Kim, J. H., Kwon, O. C., Son, E. S., Lee, C. S. and Park, Y. J. 2016. Effects of ganodermanondiol, a new melanogenesis inhibitor from the medicinal mushroom Ganoderma lucidum. Int. J. Mol. Sci. 17, 1798.？
Kim, T. J. 1996. Korean resources plants III. pp. 230, Publishing center of seoul national university, Seoul, Korea.？
Kim, Y. E., Yang, J. W., Lee, C. H. and Kwon, E. K. 2009. ABTS radical scavenging and anti-tumor effects of Tricholoma matsutake Sing. (Pine Mushroom). J. Kor. Soc. Food Sci. Nutr. 38, 555-560.？

원문보기 상세보기
Kwon, O. J. 2016. Antioxidant and tyrosinase inhibitory activities of immature fruits of Malus pumila cv. Fuji. Kor. J. Food Preserv. 23, 585-590.？

원문보기 상세보기
Lee, J. Y., Joo, D. H., Yoo, D. H. and Chae, J. W. 2017. Anti-inflammatory activities verification of Vaccinum oldhami fruit ethanol extracts on RAW 264.7. J. Life Sci. 27, 417-422.？

원문보기 상세보기
Lee, T. B. 1986. Illustrated flora of Korea. pp. 603, Hyangmunsa, Seoul, Korea.？
Lee, T. G., Hyun, S. W., Lee, I. S., Park, B. K., Kim, J. S. and Kim, C. S. 2018. Antioxidant and α-glucosidase inhibitory activities of the extracts of Aster koraiensis Leaves. Kor. J. Medicinal Crop Sci. 26, 382-390.？

원문보기 상세보기
Lee, W. T. 1996. Coloured standard illustrations of Korean plant. pp. 268, Academy Publishing Co., Seoul, Korea.？
Lee, Y. N. 1997. Flora of Korea. pp. 590-592, Kyo-Hak Publishing, Seoul, Korea.？
Oboh, G., Agunloye, O. M., Adefegha, S. A., Akinyemi, A. J. and Ademiluyi, A. O. 2015. Caffeic and chlorogenic acids inhibit key enzymes linked to type 2 diabetes(in vitro): A comparative study. J. Basic Clin. Physiol. Pharmacol. 26,165-170.？
Park, J. G., Karmer, B. S., Steinberg, S. M., Carmichael, J., Collins, J. M., Minna, J. D. and Gazdar, A. F. 1987. Chemosensitivity testing of human colorectal carcinoma cell lines using a tetrazolium-based colorimetric assay. Cancer Res. 47, 5875-5879.？

상세보기
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26, 1231-1237.？

상세보기
Yagi, A., Kanbara, T. and Morinobu, N. 1987. Inhibition of mushroom-tyrosinase by aloe extract. Planta Med. 53, 515-517.？

상세보기
Yu, M. H., Im, H. G., Lee, H. J., Ji, Y. J. and Lee, I. S. 2006. Components and their antioxidative activities of methanol extracts from sarcocarp and seed of Zizyphus jujuba var. inermis Rehder. Kor. J. Food. Sci. Technol. 38, 128-134.？
Yun, H. J., Lim, S. Y., Hur, J. M., Jeong, J. W., Yang, S. H. and Kim, D. H. 2007. Changes of functional compounds in, and texture characteristics of, apples, during post-irradiation storage at different temperatures. Kor. J. Food Preserv. 14, 239-246.？
Zaro, M. J., Keunchkarian, S., Chaves, A. R., Vicente, A. R. and Concellon, A. 2014. Changes in bioactive compounds and response to postharvest storage conditions in purple eggplants as affected by fruit developmental stage. Postharvest Biol. Technol. 96, 110-117.

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증