[논문]기능성 단옥수수 품종 육성을 위한 자식계통의 카로티노이드 함량 및 항산화 활성 평가

하준영; 신성휴; 고영삼; 배환희; 김상곤

doi:10.7740/kjcs.2023.68.2.059

기능성 단옥수수 품종 육성을 위한 자식계통의 카로티노이드 함량 및 항산화 활성 평가
Assessing Carotenoid Levels and Antioxidant Properties in Korean Sweet Corn Inbred Lines to Develop High-Quality Sweet Corn Varieties through Breeding 원문보기

Korean journal of crop science = 韓國作物學會誌, v.68 no.2, 2023년, pp.59 - 68

하준영 (농촌진흥청 국립식량과학원 중부작물부) , 신성휴 (농촌진흥청 기획조정관실 혁신행정법무담당관실) , 고영삼 (농촌진흥청 국립식량과학원 중부작물부) , 배환희 (농촌진흥청 국립식량과학원 중부작물부) , 김상곤 (경남항노화연구원 항노화연구실)

초록
AI-Helper

본 연구는 국립식량과학원에서 육성한 단옥수수 자식계통의 phytochemical 함량과 항산화 활성을 평가하여 고기능성 단옥수수 품종 육성을 위한 기초 자료로 이용하고자 수행하였다. 1. Carotenoid 함량이 가장 높은 계통은 KSE34 (1239.3±6.4 mg 100 g^-1), flavonoid 함량이 가장 높은 계통은 KSE6(68.6±9.6 mg CE 100 g^-1)으로 나타나 각 계통마다 화합물의 함량이 다양하였다. Phenolic compound 함량은 대부분의 단옥수수 자식계통에서 풍부하게 나타났으며, KSE8과 KSE25가 가장 높은 함량을 가진 계통이었다. 2. DPPH와 ABTS 라디칼 소거능으로 평가한 국내 단옥수수 자식계통의 항산화 활성 중 DPPH 활성은 계통 간에 다양하였으나, ABTS 활성은 차이가 적게 나타났다. KSE30과 KSE6이 각각 108.4±9.6 mg TE 100 g^-1, 104.2±12.8 mg TE 100 g^-1로 가장 좋은 DPPH 활성을 보인 계통이었으며 KSE47, KSE4, KSE21은 낮은 활성을 보였다. 3. 계층 군집 분석과 주성분 분석을 사용하여 단옥수수 자식계통의 농업적 특성을 비교하고 분류하였다. Carotenoid, polyphenol, flavonoid 함량이 높고 항산화 활성이 우수한 4개 계통(KSE6, KSE30, KSE34, KSE40)이 선발되었고, 향후 조합 능력 평가를 거쳐 고기능성 단옥수수 품종 개발에 활용할 것이다.

Abstract ▼ AI-Helper

Sweet corn is widely consumed due to its high nutritional content and diverse phytochemical composition, including carotenoids and phenolic compounds, which have several benefits for human health. This study aims to identify breeding materials for developing high-functional sweet corn varieties by evaluating the phytochemical and antioxidant activities of 37 Korean sweet corn inbred lines. The results revealed genetic variation in various components, such as carotenoid content (range of 120.7~1239.3 mg 100 g-1), polyphenol content (490.5~740.6 mg gallic acid equivalent 100 g-1), and flavonoid content (7.3~68.6 mg catechin equivalent 100 g-1). In addition, the free radical scavenging capacity, measured using 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid), also varied among the inbred lines. Therefore, in this study, we identified Korean sweet corn inbred lines with high phytochemical content and excellent antioxidant activity. The development of sweet corn varieties with improved functionality is expected to further expand the role of sweet corn as a source of antioxidants in the Korean diet.

주제어

표/그림 (7)

표 Table 1. List of Korean sweet corn inbred lines evaluated for carotenoid contents and antioxidant activities.
그림 Fig. 1. Visible colors and color parameters of 37 inbred lines of Korean sweet corn. (A) Dried grains, (B) Grain powders, (C) Color parameters of the dried grain, and (D) Color parameters of the grain powder. The color parameters are represented by Hunter's color L*, a*, and b*, representing the degree of lightness and darkness, redness and greenness, and yellowness and blueness, respectively. Individual dots represent the measured values, and the significance between inbred lines was tested using ANOVA. Different letters indicate significant differences between inbred lines at the 95% confidence level.
그림 Fig. 2. Phytochemical contents of Korean sweet corn inbred lines. (A) Carotenoids, (B) Polyphenols, and (C) Flavonoids. The experiments were performed three times, and the results are expressed as mean±standard deviation. The significance between the inbred lines was evaluated using one-way ANOVA (p < 0.05), and different letters indicate significant differences based on the Post hoc Duncan's multiple range test. Red dots represent the measurements.
그림 Fig. 3. Antioxidant activity of Korean sweet corn inbred lines. (A) DPPH and (B) ABTS. All experiments were performed in triplicate, and results were expressed as mean±standard deviation. Statistical significance among inbred lines was evaluated using one-way ANOVA (p < 0.05) followed by Duncan's multiple range test. Statistical significance according to Post hoc test is indicated by different letters. The experimental values are shown as red dots.
그림 Fig. 4. Pearson correlation coefficient (r) matrix for phytochemicals and antioxidant activity of Korean sweet corn inbred lines. (A) Correlation of carotenoid contents with color parameters and (B) Correlation of carotenoids, polyphenols, and flavonoids with free radical scavenging capacity. Positive and negative correlations are represented by red and blue colors, respectively. Asterisks (*, **, and ***) indicate statistically significant correlations at p < 0.05, 0.01, and 0.001, respectively.
그림 Fig. 5. Hierarchical cluster analysis heatmap of phytochemical contents and antioxidant activities in Korean sweet corn inbred lines. Pearson's correlation distance and Ward clustering were used to visualizing the hierarchical clustering of mean values.
그림 Fig. 6. A score plot (A) and biplot (B) by principal component analysis (PCA) of 37 Korean sweet corn inbred lines based on carotenoid contents and four antioxidant activities. Each number in the score plot represents a sample within the inbred line (for example, KSE1 is visualized as 1.1, 1.2, and 1.3, where .1, .2, and .3 indicate the three replicates of each sample).

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저자의 다른 논문 :

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동의어: Packet Collision Rate

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