[논문]갓과 흑미강 혼합물의 항산화 활성 및 지방세포 억제 효과

임지현; 임준석; 한웅호; 문효; 오건; 조근희; 최선일; 황운상; 이옥환

doi:10.9799/ksfan.2023.36.4.244

갓과 흑미강 혼합물의 항산화 활성 및 지방세포 억제 효과
Antioxidant Activity and Anti-Obesity Effects of Mixture of Brassica juncea and Black Rice Bran 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.36 no.4, 2023년, pp.244 - 252

임지현 (강원대학교 식품환경융합학과) , 임준석 (강원대학교 바이오산업공학부 식품생명공학전공) , 한웅호 (강원대학교 식품환경융합학과) , 문효 (강원대학교 식품환경융합학과) , 오건 (강원대학교 식품환경융합학과) , 조근희 (강원대학교 바이오산업공학부 식품생명공학전공) , 최선일 (강원대학교 농업생명과학연구원) , 황운상 ((주)에스티알 바이오텍) , 이옥환 (강원대학교 식품환경융합학과)

Abstract ▼ AI-Helper

This study was conducted to provide basic data on the antioxidant activity, inhibition of adipocyte differentiation and reactive oxygen species (ROS) production of a mixture of Brassica juncea extract (BJE) and fermented black rice fraction (BRF). We investigated the total phenol content, total flavonoid content, antioxidant effects (DPPH radical scavenging, ABTS radical scavenging, reducing power, FRAP and ORAC assay) and anti-obesity activity of the mixture in 3T3-L1 cells. Our results showed that the total phenol and flavonoid content increased with increasing BRF mixture ratio. The antioxidant activity increased as the BRF mixture ratio increased. In addition, BJE and BRF mixtures did not show any cytotoxicity during the 3T3-L1 differentiation period. During adipocyte differentiation, BJE and BRF mixtures significantly inhibited lipid accumulation and ROS production compared to the control group. These results warrant further experiments to develop an anti-obesity functional food using a mixture of BJE and BRF.

주제어

표/그림 (6)

표 Table 1. Total polyphenol and flavonoid contents of BRM1, BRM2, BRM3
그림 Fig. 1. Antioxidant activity of BRM1, BRM2 and BRM3 and ascorbic acid (ASA). (A) 2,2-diphenyl-1-pic-rhlhydrazyl (DPPH) radical scavenging activity, (B) 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, (C) FRAP activity, (D) reducing power, (E) Decay curve of fluorescence in the presence or absence of samples, (F) Radical scavenging activity of BRM1, BRM2 and BRM3 expressed as Trolox equivalents. Each value is expressed as the mean±S.D. of triple determination. The other letters on the bar indicate a significant difference of p<0.05 in the Duncan multi-range test.
그림 Fig. 2. Effect of BRM1, BRM2, and BRM3 on cell viability on 3T3-L1 preadipocytes. Each value is expressed the mean±S.D. of the results from five different plates (n=5). Statistical analysis was performed using the one-way ANOVA (p<0.05).
그림 Fig. 3. Effect of BRM1, BRM2, and BRM3 on lipid accumulation during adipogenesis of 3T3-L1 preadipocytes. Oil red O staining at day 6. Lipid accumulation determined by optical density at 490 nm. The result is presented as the mean±S.D. of 3 independent in triplicate. Bars with different letters indicate significantly different at p<0.05 by Duncan's multiple range test.
표 Table 2. Correlation coefficients among total phenol content (TPC), total flavonoid content (TFC), DPPH radical scavenging (DPPH), ABTS radical scavenging (ABTS), FRAP, reducing power (RP) and ORAC vale of mixtures of Brassica juncea and black rice bran
그림 Fig. 4. Effect of BRM1, BRM2 and, BRM3 on ROS production during adipogenesis of 3T3-L1 preadipocytes. Dark-blue formazan was dissolved and the optical density was determined at 570 nm. The result is presented as the mean±S.D. of 3 independent in triplicate. Bars with different letters indicate significantly different at p<0.05 by Duncan's multiple range test.

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