[논문]잎들깨 유전자원의 기능성분 및 항산화 활성

안연주; 김정인; 김상우; 김성업; 오은영; 이정은; 이은수; 유은애; 성정숙; 이명희; 김춘송; 김민영

doi:10.9799/ksfan.2023.36.5.379

잎들깨 유전자원의 기능성분 및 항산화 활성
Functional Components and Antioxidant Activities of Perilla Leaf Genetic Resource 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.36 no.5, 2023년, pp.379 - 386

안연주 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과 산학협동과정) , 김정인 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) , 김상우 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) , 김성업 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) , 오은영 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) , 이정은 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) , 이은수 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) , 유은애 (농촌진흥청 국립농업과학원 농업유전자원센터) , 성정숙 (농촌진흥청 국립농업과학원 농업유전자원센터) , 이명희 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) , 김춘송 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) , 김민영 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과)

Abstract ▼ AI-Helper

The objective of this study was to investigate the distribution of functional compounds in perilla leaves of various genetic resources and their antioxidant activities. A comprehensive analysis of functional compounds was conducted for 90 genetic resources, focusing on total polyphenol content (TPC), total flavonoid content (TFC), individual phenolic content (IPC), and lutein. Their antioxidant activities were then analyzed based on their radical scavenging capacity using ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl). The TPC content exhibited a range of 13.19 to 35.85 mg gallic acid equivalent/g, whereas the TFC content varied from 11.74 to 46.51 mg catechin equivalent/g. Total IPC was detected in a range of 6,310.98 to 40,491.82 ㎍/g. Lutein was detected at levels between 70.97 and 597.97 ㎍/g. ABTS and DPPH radical scavenging activities of perilla leaves ranged from 30.39 to 58.58 mg trolox equivalent (TE)/g and from 7.74 to 46.56 mg TE/g, respectively. Furthermore, correlation analysis demonstrated that rosmarinic acid, a phenolic acid, exhibited a significantly positive correlation with antioxidant activity. These findings suggest that various genetic resource of perilla leaves could effectively mediate antioxidant capacity. Results of this study provide valuable information for use of perilla leaves in Korea as functional food materials.

주제어

표/그림 (7)

표 Table 1. The analytical conditions of HPLC for identification and determination of phenolic compounds
표 Table 2. The analytical conditions of HPLC for identification and determination of lutein
표 Table 3. Summary of functional compounds and antioxidant activities
그림 Fig. 1. HPLC chromatograms of phenolic compounds from high caffeic acid resource IT318022 and low caffeic acid resource IT274644: (1) caffeic acid, (2) rosmarinic acid and (3) luteolin.
그림 Fig. 2. Correlation analysis between functional compounds and antioxidant activities. Correlation analysis was performed based on pearson's correlation coefficient. TPC: total polyphenol content, TFC: total flavonoid content.
그림 Fig. 3. Heatmap including cluster analysis according to functional compounds and antioxidant activities in the perilla leaves of genetic resources. A total of eight variables were used in the analysis, which were divided into three clusters. Each cluster is colored vlue (I), red (II) and green (III). TPC: total polyphenol content, TFC: total flavonoid content.
표 Table 4. Summary of principal component analysis (PCA) statics and eigenvalues for functional compounds and antioxidant activities

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