[논문]합성 접촉페로몬을 이용한 꽃노랑총채벌레의 성충 행동 교란과 이를 이용한 방제기술

박현제; 김철영; 정성채; 김영운; 김용균

doi:10.5656/ksae.2023.08.0.030

합성 접촉페로몬을 이용한 꽃노랑총채벌레의 성충 행동 교란과 이를 이용한 방제기술
Behavioral Disruption of Frankliniella occidentalis Adults by a Synthetic Contact Pheromone and its Application to Control the Insect Pest 원문보기

한국응용곤충학회지 = Korean journal of applied entomology, v.62 no.3, 2023년, pp.161 - 169

박현제 (안동대학교 식물의학과) , 김철영 (안동대학교 식물의학과) , 정성채 ((주)에이디) , 김영운 ((주)에이디) , 김용균 (안동대학교 식물의학과)

초록
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소형 곤충으로 좁은 틈새에 있는 총채벌레는 종종 살충제 살포에 노출되기 어렵다. 이에 접촉페로몬을 처리하여 은둔행동을 막으려는 행동교란 방제 전략을 세웠다. 꽃노랑총채벌레(Frankliniella occidentalis)에서 밝혀진 접촉페로몬은 7-methyltricosane (7TM)으로 본 연구에서는 먹이에 이 물질을 처리하여 행동 교란 유무를 생물검정하였다. 이 접촉페로몬은 유충에게 영향을 주지 않았지만 수컷 성충으로 하여금 처리된 먹이로부터 회피하려는 행동을 유발하였다. 반면에 암컷 성충에게는 오히려 7TM이 처리된 지역으로 이동하는 행동을 유발하였다. 동일한 접촉페로몬에 대해서 대만총채벌레(Frankliniella intonsa)에서도 유사한 행동 변화가 관찰되었다. 이러한 7TM에 기인한 총채벌레의 행동 변화를 살충제 처리와 연결하여 고추를 가해하는 총채벌레류를 대상으로 방제효과를 검정하였다. 스피네토람 살충제 단독 처리에 비해 7TM과 혼합하여 처리하면 총채벌레류 방제효율이 증가하였다. 흥미로운 점은 7TM 단독 처리로도 대만총채벌레 밀도에 일부 감소 효과를 보여 이 물질이 총채벌레의 행동교란을 주는 것을 뒤받침하였다. 본 연구는 접촉페로몬 처리로 총채벌레의 살충제 회피 행동을 줄여 방제효과를 높인 새로운 해충방제기술을 제시한다.

Abstract ▼ AI-Helper

Thrips are usually not exposed to insecticide spray due to hiding at holes, gaps, or crevices of host plants with their relatively small body sizes. This study devised a strategy to use a contact pheromone to suppress the seclusive behavior of the thrips, A contact pheromone identified as 7-methyltricosane (7TM) in the western flower thrips, Frankliniella occidentalis, was added to the diet to test the preference of the thrips. Although 7TM did not change the larval behavior, it significantly induced the avoiding behavior in male adults. In contrast, the contact pheromone was preferred by the female adults. Similar behavioral changes were also observed in another flower thrips, Frankliniella intonsa. Based on the behavioral changes induced by the contact pheromone, a mixture of 7TM and insecticide was applied to thrips infesting hot peppers in field conditions. Compared to spinetoram treatment, its mixture treatment with 7TM enhanced the control efficacy against thrips. Interestingly, 7TM treatment alone also gave slight reduction in F. intonsa density, suggesting a behavioral disruption of thrips by the contact pheromone. This study suggests a novel technology to control insect pests using contact pheromone by suppressing the seclusive behavior to avoid exposure to insecticide spray.

주제어

표/그림 (4)

그림 Fig. 1. Chemical synthesis of 7-methyltricosane. (A) A schematic summary of the synthesis. Reagents and conditions in each synthetic step are (a) triphenylphosphine, toluene, reflux, (b) 3,4-dihydropyran, pyridinium p-toluenesulfonate, tetrahydrofuran, room temperature, (c) Pyridinium chlorochromate, dichloromethane, 0°C, (d) Potassium tert-butoxide, tetrahydrofuran, room temperature, (e) p-toluenesulfonic acid, methanol, and (f) Palladium on carbon, methanol. Compounds in the synthetic scheme are 7-methyltricosane (1), 1-bromotetrade cane (2), triphenyl(tetradecyl)phosphonium bromide (3), 1-bromobutane (4), buthyltriphenylphosphonium bromide (5), 3-methyl-1,5- pentanediol (6), 3-methyl-5-((tetrahydro-2H-pyran-2-yl)oxy)pentan-1-ol (7), 3-methyl-5-((tetrahydro-2H-pyran-2-yl)oxy)pentanal (8), (Z)-2 ((3-methylnonadec-5-en-1-yl)oxy)tetrahydro-2-H-pyran (9), (Z)-3-methylnonadec-5-en-1-ol (10), (Z)-3-methylnonadec-5-enal (11), and (4Z,9Z)-7-methyltricosa-4,9-diene (12). (B) Gas chromatogram of the synthesized 7-methyltricosane.
그림 Fig. 2. Behavioral test in an arena (A) of two thrips species of F. occidentalis (B) and F. intonsa (C). In an arena, 7-methyltricosane (7TM) was treated on filter paper disc on soy bean diet. Control diet was treated with hexane solvent. After 10 min, the number of thrips reached to each diet were counted. Each treatment was replicated four times by exchanging the treatment and control positions. Asterisk stands for significant difference at Type I error = 0,05 (LSD test) while 'NS' stands for no significant difference.
그림 Fig. 3. Control effect of a mixture of 7-methyltricosane ('7TM', 100 'g/ml) and spinetoram ('SP', 25 μg/ml) against thrips infesting hot peppers in an open field. Control ('CON') represents no spray. A hot pepper was an experimental unit. A total number of thrips were counted in each plant at 0, 1, and 3 days after treatment. Each treatment was installed by a randomized block design with three replications.
표 Table 1. Control efficacy of a mixture of 7-methyltricosane (7MT) and spinetoram against thrips infesting hot peppers in an open field at three days after treatment. Survival rate was calculated in percentage by dividing the thrips number at 3 days after treatment with the initial number. Three replications are denoted by I, II, and III

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