[논문]화장품 소재 개발을 위한 원지 (Polygala tenuifolia), 백지(Angelica dahurica) 및 꽃향유 (Elsholtzia splendens) 추출물의 혼합 비율 최적화

정서아; 송가현; 박수인; 정연옥

doi:10.17703/jcct.2023.9.6.993

화장품 소재 개발을 위한 원지 (Polygala tenuifolia), 백지(Angelica dahurica) 및 꽃향유 (Elsholtzia splendens) 추출물의 혼합 비율 최적화
Optimization of mixing ratio of Polygala tenuifolia, Angelica dahurica and Elsholtzia splendens extracts for cosmetic material development 원문보기

Journal of the convergence on culture technology : JCCT = 문화기술의 융합, v.9 no.6, 2023년, pp.993 - 1000

정서아 ((주)수이케이 연구원) , 송가현 ((주)수이케이 연구원) , 박수인 (을지대학교 미용화장품과학과) , 정연옥 ((주)수이케이)

초록
AI-Helper

최근 화장품 소재로서 환경친화적인 식물성 유래 천연물질이 주목받고 있으며, 천연물의 다양한 생리활성에 대한 연구가 활발히 이루어지고 있다. 본 연구에서는 다양한 생리활성이 있다고 알려져 있는 약용식물 원지 (P olygala tenuifolia), 백지 (Angelica dahurica), 꽃향유 (Elsholtzia splendens) 3종 추출물의 배합 비율을 달리하여 항산화, 항염증, 보습 및 항균 효능을 조사하였다. 배합 비율은 7가지 조건 (M1, 1:1:1; M2, 0.5:1.5:1; M3, 1.5:0.5:1; M4, 0.1:0.95:0.95; M5, 0.5:0.5:2; M6, 0.95:1.95:0.1; M7, 1.45:0.1:1.45)으로 설정하여 추출하였고, 화장품 소재로 활용하기위해 최적의 혼합 비율을 확인하였다. DPPH 및 ABTS radical 소거 활성은 M6 1,000 ㎍/mL에서 각각 75.37% 및99.19%의 소거능을 나타냈다. M6 200 ㎍/mL 농도에서는 염증반응을 유도한 lipopolysaccharide 처리군 대비 50% 의 Nitric oxide 생성 억제 효과를 보였다. M3와 M6은 50 ㎍/mL 농도에서 대조군에 비해 hyaluronic acid 생성량이 각각 1.47배, 1.49배 높은 것을 확인하였다. Disc diffiusion 테스트에서는 M6 8 ㎍/mL에서 clear zone이 9.75 mm로 나타나 Staplylococcus aureus 균주의 생장 억제를 확인하였다. 이상의 결과로 원지, 백지, 꽃향유 혼합 추출물은 화장품의 기능성 천연소재로 사용 가능할 것이라 판단된다.

Abstract ▼ AI-Helper

Recently, enviromentally friendly natural substances derived from plants have been attracting attention as cosmetic materials, and research on various physiological activities of natural substances is being actively conducted. This study investigated the antioxidant, anti-inflammatory, moisturizing, and antibacterial effects of three types of extracts of mixtures containing different mixing ratios, Polygala tenuifolia, Angelica dahurica, and Elsholtzia splendens, known to have various physiological activities. The mixing ratio is 7 conditions (M1, 1:1:1; M2, 0.5:1.5:1; M3, 1.5:0.5:1; M4, 0.1:0.95:0.95; M5, 0.5:0.5:2; M6, 0.95 :1.95:0.1; M7, 1.45:0.1:1.45), and the optimal mixing ratio was confirmed for use as a cosmetic material. DPPH and ABTS radical scavenging activities showed scavenging abilities of 75.37% and 99.19%, respectively, at 1,000 ㎍/mL of M6. At a concentration of 200 ㎍/mL of M6, it showed 50% of nitric oxide production inhibition compared to the lipopolysaccharide-treated that induced an inflammatory response. It was confirmed that M3 and M6 produced hyaluronic acid 1.47 and 1.49 times higher than the control at a concentration of 50 ㎍/mL, respectively. Through the disc diffiusion test, the clear zone was 9.75 mm at 8 ㎍/mL of M6, confirming the inhibition of growth of staplylococcus aureus strain. Based on the above results, it is believed that the mixed extract of Polygala tenuifolia, Angelica dahurica, and Elsholtzia splendens can be used as a functional natural material for cosmetics.

주제어

표/그림 (7)

표 표 1. 원지, 백지, 꽃향유 추출물의 혼합 비율 Table 1. Mixture ratio of Polygala teuifolia, Angelicae dahurica, Elsholtzia splendens extract
그림 그림 2. Raw 264.7 세포 생존율(%) Figure 2. Raw 2647.7 cell viability treated with Mixture extract of Polygala teuifolia, Angelica dahurica, Elsholtzia splendens
그림 그림 3. Raw 264.7 세포의 NO 생성량(%) Figure 3. The inhibitory effect Mixture extract of Polygala teuifolia , Angelica dahurica, Elsholtzia splendens on NO production in LPS treated Raw 267.7 cell, ^###P<0.001 vs. CTL, *P<0.1 and ***P<0.001 vs. LPS, CTL: non-treated control, LPS: LPS treated control.
그림 그림 4. HaCaT 세포 생존율(%) Figure 4. HaCaT cell viability treated with Mixture extract of Polygala teuifolia, Angelica dahurica, Elsholtzia splendens.
그림 그림 1. 추출물 혼합 비율에 따른 DPPH (A) and ABTS(B) radical 소거 활성 Figure 1. DPPH (A) and ABTS(B) radical scavenging activity according to extracts mixture ratio.
그림 그림 5. 원지, 백지, 꽃향유 혼합 추출물이 히알루론산 생성 에 미치는 영향 Figure 5. Effect of Mixture extract of Polygala teuifolia, Angelica dahurica, Elsholtzia splendens on production of hyaluronic acid, *P<0.1 and **P<0.01 vs. CTL.
표 표 2. 원지, 백지, 꽃향유 혼합 추출물의 Staphylococcus aureus에 대한 clear zone 직경 측정 결과 Table 2. Clear zone diameter measurement results for Staphylococcus aureus of Mixture extract ratio of Polygala teuifolia, Angelica dahurica, Elsholtzia splendens

참고문헌 (35)

Y.K. Choi and B.J. Ha, "Comparison of the？Physicochemical Characteristics and Antibacterial Efficiencies of the Extracts Obtained from？Artemisia princeps var. orientalis," Asian Journal？of Beauty & Cosmetology, Vol. 12, No. 5, pp.？685-692, October 2014.
H.S. Kim, J.M. Cheon, D.H. Kwon, E.O. Choi,？M.J. Kim, Y.H. Choi, B.W. Kim and H.J. Hwang,？"Inhibitory Effects of Myelophycus simplex？Papenfuss Methanol Extract on Melanogenesis in？B16F10 Melanoma Cells," Journal of The Korean？Society of Food Science and Nutrition, Vol. 46,？No. 1, pp. 34-38, January 2017. DOI:？https://doi.org/10.3746/jkfn.2017.46.1.034

원문보기 상세보기
K.D. Kim, "Research of Efficacy & Stability？about Mixed Medicinal Plants Extracts," Jounal？of The Korean Society of cosmetology, Vol. 13,？No. 2, pp. 601-608, July 2007.
Ahn, J.H., the Antioxidant, Antimicrobial and？Anti-Inflammatory Effect of Natural Extract？Mixtures, Ph.D. Thesis. University of Kosin,？Busan, Korea., 2018.
B.H. May, C. Lu, Y. Lu, A.L. Zhang and C.C？Xue, "Chinese Herbs for Memory Disorders: A？Review and Systematic Analysis of Classical？Herbal Literature," Journal of Acupuncture &？Meridian Studies, Vol. 6, No. 1, pp. 2-11,？February 2013. DOI: https://doi.org/10.1016/j.jams.2012.11.009

상세보기
R.O. de Campos, A.R. Santos, Z.R. Vaz, T.R.？Pinheiro, M.G. Pizzolatti and V. Cechinel Filho,？"Antinociceptive Properties of the Hydroalcoholic？Extract and Preliminary Study of a Xanthone？Isolated from Polygala cyparissias(Polygalaceae),"？Life Sciences, Vol. 61, No. 16, pp. 1619-1630,？September 1997. DOI: https://doi.org/10.1016/S0024-3205(97)00741-8

상세보기
J.Y. Hwang, Y.X. Wu, D.I. Hwang, S.J. Bae and？T. Kim, "Anti-obesity Effect of Polygala？tenuifolia," Korean Journal of Food Preservation,？Vol. 21, No. 1, pp. 97-106, February 2014. DOI:？https://doi.org/10.11002/kjfp.2014.21.1.97

원문보기 상세보기
K.C. Huang, The Pharmacology of Chinese herbs,？CRC press LLC Florida, pp. 286-287, 1999.
H.Y. Kim, C. Park, Y.H. Choi and W.D. Hwang,？"The Protective Effect of Ethanol Extract of？Polygalae Radix against Oxidative？Stress-Induced DNA Damage and Apoptosis in？Chang Liver Cells," Journal of Korean Medicine？for Obesity Research, Vol. 19, No. 1, pp. 1-11,？May 2019. DOI: https://doi.org/10.15429/jkomor.2019.19.1.1

원문보기 상세보기
H.S. Oh and B.W. Kim, "Anti-inflammatory？activity of the Water Extract of Root of Polygala？tenuifolia Willd.," The Journal of Internal Korean？Medicine, Vol. 34, No. 2, pp. 204-214, June 2013.
D.S. Lee, H.G. Choi, B. Li, K.S. Kim, S.A. Kim,？S.K. Chon, J.M. Rho, K.M. Kim, J.H. Han, G.S.？Jeong and Y.C. Kim, "Neuroprotective Effect of？the Acid Hydrolysis Fraction of the Root of？Polygala tenuifolia." Korean J Oriental Physiology？& Pathology, Vol. 25, No. 4, pp. 628-634, August？2013.
S.H. Kim and D.K. Chung, "Nootropic and？Anti-amnestic Effect of PPA on？Scopolamine-induced Cognitive Impairment in？Mice". J of Oriental Neuropsychiatry. Vol. 22, No.？4, pp. 185-199, October 2011.
H.S. Kim, and H.J. Choi, "Studies on Essential？Oils of Plants of Angelica genus in Korea(III),"？Korean Journal of Pharmacognosy, Vol. 21, No. 2,？pp. 121-125, June 1990.
E.Y. Joo and W.J. Kang, "Analysis on the？Components of the Angelica dahurica Root," the？Korean Society of Food Preservation, Vol. 12, No.？5, pp. 476-481, October 2005.
S.H. Kim, S.S. Kang and C.M. Kim, "Coumarin？Glycosides from the Roots of Angelica dahurica,"？Archives of Pharmacal Research, Vol. 15, No. 1,？pp. 73-77, March 1992.

원문보기 상세보기
Y.S. Kwon and C.M. Kim, "Coumarin Glycosides？from the Roots of Angelica dahurica," Korean？Journal of Pharmacognosy, Vol. 24, No. 4, pp.？221-224, December 1992.
M.H. Jin, M.H. Jung, Y.H. Lim, S.H. Lee, S.J.？Kang and W.G. Cho, "Promoting Synthesis of？Collagen from Angelica dahurica Root," Korean？Journal of Pharmacognosy, Vol. 35, No. 4, pp.？315-319, December 2004.
D.K. Kim, J.P. Lim, J.H. Yang, D.O. Eom, J.S.？Eun and K.H. Leem, "Acetylcholinesterase？Inhibitors from the Roots of Angelica dahurica,"？Archives of Pharmacal Research, Vol. 25, No. 6,？pp. 856-859, December 2002.

원문보기 상세보기
C.M. Kim, Y.S. Kwon and S.Y. Choi,？"Antithrombotic Effect of the BuOH Soluble？Fraction of Angelica dahurica Root," Korean？Journal of Pharmacognosy, Vol. 26, No. 1, pp.？74-77, March 1995.
S.Y. Ryu, J.C. Kim, Y.S. Kim, H.T. Kim, W.K.？Kim, G.J. Choi, J.S. Kim, S.W. Lee, J.H. Heor？and K.Y. Cho, "Antifungal Activities of？Coumarins Isolated from Angelica gigas and？Angelica dahurica Against Plant Pathogenic Ffungi," The Korean Society of Pesicide Science,？Vol. 5, No. 3, pp. 26-35, September 2001.
H.S. Han, S.S. Lim, K. Suzuki, S.H. Jung, S.？Lee, Y.S. Lee, K.H. Shin and K. Ohuchi,？"Inhibitory Effects of Furanocoumarins Isolated？from the Roots of Angelica dahurica on？Prostaglandin E2 Production," Planta Medica, Vol.？69, No. 5, pp. 408-412, May 2003. DOI:？https://doi.org/10.1055/s-2003-39702.

상세보기
C.B. Lee, Korean Dictionary of Plant,？Hyangmunsa, pp. 660, 1999.
M.S. Chung and M.S. Lee, "Development of？Elsholtzia splendens-Flavored Oils and Analysis？of Flavor Pattern Using Electronic Nose," Korean？Journal of Food and Cookery Science, Vol. 18,？No. 4, pp. 455-460, August 2002.
K. Bae, the medicinal plants of Korea, Kyohaksa？Co., pp. 435, 2000.
K.H. Sohn, J.S. Song, Y.A. Chae and K.S. Kim,？"The Growth and Analysis of Essential oil Of？Elsholtzia splendens Nakai," Korean Society for？Horticultural Science, Vol. 40, No. 2, pp. 271-275,？April 1999.
S.J. Kim and G.H. Kim, "Identification for？Flavones in Different Parts of Cirsium？japonicum," Preventive Nutrition and Food？Science, Vol. 8, No. 4, pp. 330-335, December？2003. DOI: https://doi.org/10.3746/jfn.2003.8.4.330

원문보기 상세보기
Kang, J.R., Antioxidant Activity and Tyrosinase？Inhibitory Activity of Elscholtziae Herba, Ph.D.？thesis. Chung-Ang University, Seoul, Korea.,？2004
D.W. Kim, K.H. Son, H.W. Chang, K.H. Bae, S.S？Kang and H.P. Kim, "Anti-inflammatory Activity？of Elsholtzia splendens," Archives of Pharmacal？Research, Vol. 26, No. 3, pp. 232-236, March？2003.

원문보기 상세보기
M.S. Blois, "Antioxidant Determinations by the？Use of a Stable Free Radical," Nature, Vol. 181,？No. 4617, pp. 1199-1200, April 1958. DOI:？https://doi.org/10.1038/1811199a0

상세보기
S.H. Heo, S.I. Park, G.M. An and M.S. Shin,？"Physiological Activity of Robinia pseudo acacia？Leaf Extracts and Enhancement of Skin？Permeation Using Polymer Micelles and Cell？Penetrating Peptide," The Journal of the？Convergence on Culture Technology, Vol. 5, No.？3, pp. 271-282, August 2019. DOI:？https://doi.org/10.17703/JCCT.2019.5.3.271

원문보기 상세보기
R. Re, N. Pellegrini, A. Proteggente, A. Pannala,？M. Yang and C. Riceevans, "Antioxidant Activity？Applying an Improved ABTS Radical Cation？Decolorization Assay," Free Radical Biology &？Medicine, Vol. 26, No. 9-10, pp. 1231-1237, May？1999. DOI: https://doi.org/10.1016/S0891-5849(98)00315-3

상세보기
M.O. Ko, H.J. Kang, J.H. Hwang and K.W.？Yang, "Screening of the Antibacterial Effects by？Ethanol Extracts from Natural Plant in Jeju？Against Propionibacterium Acnes," Journal of？Society of Cosmetic Scientists of Korea. Vol. 44,？No. 1, pp. 59-66, March 2018. DOI:？https://doi.org/10.15230/SCSK.2018.44.1.59

원문보기 상세보기
V. Bondet, W. Brand-Williams and C. Berset,？"Kinetics and Mechanisms of Antioxidant？Activity Using the DPPH Rree Radical Method,"？Lebensm Wiss Technol, Vol. 30, No. 6, pp.？609-615, September 1997. DOI: https://doi.org/10.1006/fstl.1997.0240

상세보기
E. Papakonstantinou, M. Roth and G.？Karakiulakis, "Hyaluronic acid: A Key Molecule？in Skin Aging," Dermato Endocrinology, Vol. 4,？No. 3, pp. 253-258, July 2012. DOI:？https://doi.org/10.4161/derm.21923

상세보기
J.J. Biemer, "Antimicrobial Susceptibility Testing？by the Kirby-Bauer Disc Diffusion Method,"？Annals of Clinical & Laboratory Science, Vol. 3,？No. 2, pp. 135-140, April 1973.

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