[논문]사람 모유두세포에서 PI3K/Akt와 Wnt/β-catenine 신호전달을 경유한 저령추출물의 세포증식 효과

강리아민주; 강석종; 문연자

doi:10.6116/kjh.2024.39.1.23

사람 모유두세포에서 PI3K/Akt와 Wnt/β-catenine 신호전달을 경유한 저령추출물의 세포증식 효과
Proliferative Activity of Polyporus umbellatus Extract from Mushrooms via the PI3K/Akt and Wnt/β-catenine signaling in HHDPCs 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.39 no.1, 2024년, pp.23 - 29

강리아민주 (원광대학교 한의학전문대학원 한약자원개발학과) , 강석종 (원광대학교 대학원 식품산업융복합학과) , 문연자 (원광대학교 한의학전문대학원 한약자원개발학과)

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Objectives : Polyporus umbellatus is a medicinal mushroom that has been used for over thousands years in Chinese medicine as a powerful diuretic to relieve fluid retention and edema. Dermal papilla is located at the bottom of the hair follicle and connected to the blood vessels where it gets the nutrients and oxygen to nurture hair follicle. This study examined the mechanism through which the ethanol extract of Polyporus umbellatus (EPU) promoted the proliferation of human dermal papilla cells (HHDPCs). Methods : To estimate the proliferative effects of EPU on HHDPCs, cell viability was estimated by thiazolyl blue tetrazolium bromide (MTT) assay. Western blotting was used to investgate the activation of ERK, phosphoinositide 3-kinase (PI3K)/Akt, β-catenin, GSK-3β and heme oxygenase-1 (HO-1). Cells were treated with inhibitors of ERK and Akt prior to EPU treatment. Results : EPU promoted the proliferation of HHDPCs and the phosphorylation of ERK and Akt in dose dependent manner. However, the proliferative effect of EPU on HHDPCs was inhibited by pre-treatment of ERK inhibitor (PD98059) and Akt inhibitor (LY294002). Furthermore, EPU respectively stimulated the protein expression of β-catenin and phosphorylated GSK-3β. EPU significantly increased the protein expression levels of proliferation and cytoprotection related genes such as Bcl-2, SIRT-1, and HO-1 in cells. Conclusion : This results suggest that EPU promoted the proliferation of HHDPCs via activating PI3K/Akt and Wnt/β-catenin signaling pathway in HHDPCs.

주제어

표/그림 (6)

그림 Fig. 1. Extract of Polyporus umbellatus (EPU) accelerates the proliferation of HHDPCs. After treatment with 75, 100 and 150 ㎍ /㎖ EPU for 24 h, the cell viability of HHDPCs was measured by MTT assay. Results represent the mean ± S.D. of triplicate determinations. *p<0.05, **p<0.01 compared with the control.
그림 Fig. 2. ERK activation is involved in EPU-induced proliferation of HHDPCs. Cells were pretreated with ERK inhibitor PD98059 (20 μM) for 1 h followed by treatment with EPU (100 and 150 ㎍/㎖) or Minoxidil (MXD) (10 μM). The protein level of ERK activation was analyzed using immunoblotting after 30 min (A) and the cell viability of HHDPCs were measured by MTT assay after 24 h (B). MXD served as positive control. Results represent the mean ± S.D. of triplicate determinations. *p<0.05, **p<0.01 compared with the control.
그림 Fig. 3. PI3K/Akt pathway is involved in EPU-induced proliferation of HHDPCs. Cells were pretreated with PI3K inhibitor LY294002 (20 μM) for 1 h followed by treatment with EPU (100 and 150 ㎍ /㎖) or Minoxidil (MXD) (10 μM). The cell viability of HHDPCs were measured by MTT assay after 24 h (A) and the protein level of Akt activation was analyzed using immunoblotting after 30 min (B). MXD served as positive control. Results represent the mean ± S.D. of triplicate determinations. *p<0.05, **p<0.01 compared with the control.
그림 Fig. 4. EPU promotes β-catenin and GSK-3β activation in HHDPCs. (A) Cells were treated with EPU (100, 150 ㎍/㎖) or MXD (10 μM) for 72 h. The protein level of β-catenin and phosphorylated GSK-3β were analyzed using immunoblotting. (B) The quantification of (A) by ImageJ. Relative expression levels of β-catenin and phosphorylated GSK-3β. Results represent the mean ± S.D. of triplicate determinations. *p<0.05, **p<0.01 compared with the control.
그림 Fig. 5. EPU induces protein expression of proliferation related factors in HHDPC. Cells were treated with EPU (100, 150 ㎍/㎖) or MXD (10 μM) for 72 h. Protein expressions of Bcl-2 and SIRT-1 were assess using immunoblotting (A) and quentified by ImageJ (B). Results represent the mean ± S.D. of duplicate determinations. *p<0.05, **p<0.01 compared with the control.
그림 Fig. 6. EPU induces protein expression of HO-1 in HHDPC. Cells were treated with EPU (100, 150 ㎍/㎖) or MXD (10 μM) for 72 h. Protein expressions of HO-1 was assess using immunoblotting (A) and quentified by ImageJ (B). Results represent the mean ± S.D. of duplicate determinations. *p<0.05, **p<0.01 compared with the control.

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