[논문]백수오(白首烏)와 비주화(啤酒花) 복합물의 에스트로겐 활성과 파골세포 분화 억제효과

박동준; 이홍구; 민경인; 박형국; 진무현; 조호성

doi:10.6116/kjh.2022.37.5.1.

[국내논문] 백수오(白首烏)와 비주화(啤酒花) 복합물의 에스트로겐 활성과 파골세포 분화 억제효과
Effects of a Mixture of Cynanchi Wilfordii Radix and Humuli Lupuli Flos Extract on Estrogenic Activities and Anti-Osteoclastogenesis 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.5, 2022년, pp.1 - 8

박동준 (LG생활건강 기술연구원) , 이홍구 (LG생활건강 기술연구원) , 민경인 (LG생활건강 기술연구원) , 박형국 (LG생활건강 기술연구원) , 진무현 (LG생활건강 기술연구원) , 조호성 (LG생활건강 기술연구원)

Abstract ▼ AI-Helper

Objectives : This study aimed to investigate the synergistic effect of combining Cynanchi Wilfordii Radix extract with Humuli Lupuli Flos extract on estrogenic and anti-osteoclastogenic activity. Methods : Estrogenic effect of a mixture of Cynanchi Wilfordii Radix extract and Humuli Lupuli Flos extract (CWHL), Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin (an active ingredient of Cynanchi wilfordii Radix extract) and 8-prenylnaringenin (an active ingredient of Humuli Lupuli Flos extract) were examined by proliferation E-screen assay and expression of estrogen inducible gene, pS2 via Real Time-PCR (RT-PCR) in MCF-7 estrogen responsive cells. And their estrogenic activities were investigated how to modulate Estrogen receptor 𝛽 by binding affinity assay. Inhibitory effect of CWHL, Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin and 8-prenylnaringenin on RANKL-induced osteoclast differentiation were tested by TRAP (Tartrate-resistant acid phosphatase) staining in osteoclastogenic RAW 264.7 cells. Results : CWHL, Humuli Lupuli Flos extract and 8-prenylnaringenin accelerated the proliferation of MCF-7 and the expression of pS2 in MCF-7. CWHL, Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin and 8-prenylnaringenin bind to estrogen receptor 𝛽. CWHL, Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin and 8-prenylnaringenin inhibited RANKL-induced osteoclastogenesis in osteoclastogenic RAW 264.7. CWHL is more effective for all markers than Cynanchi Wilfordii Radix extract or Humuli Lupuli Flos extract alone. Conclusions : CWHL may a potential therapeutic agent for menopause and osteoporosis as a natural food resource. CWHL as a natural food source has therapeutic potential in cases of menopause and osteoporosis.

주제어

표/그림 (4)

그림 Fig. 1 Effect of estrogenic activity on proliferation of MCF-7 cells. Bars mean relative absorbance at 450 nm vs (-) control and present the mean ± SD. E2; 17β-estradiol, CWHL; Mixture of Cynanchi Wilfordii Radix and Humuli Lupuli Flos extract, HL; Humuli Lupuli Flos extract, CW; Cynanchi Wilfordii Radix extract. PN; 8-prenylnaringenin, CD; Caudatin. Statistical significance; (A, C and D), *p < 0.05, **p < 0.01, ***p < 0.001 vs (-) control. (B) Each alphabet groups were significantly different, p < 0.05.
그림 Fig. 2 Effect of CWHL on pS2 mRNA expression in MCF-7 cells. Bars mean relative pS2 mRNA level vs (-) control and present the mean ± SD. E2; 17β-estradiol, CWHL; Mixture of Cynanchi Wilfordii Radix and Humuli Lupuli Flos extract, HL; Humuli Lupuli Flos extract, CW; Cynanchi Wilfordii Radix extract. PN; 8-prenylnaringenin, CD; Caudatin. Statistical significance; (A) Each alphabet groups were significantly different, p < 0.05. (B) *p < 0.05, **p < 0.01 vs (-) control.
그림 Fig. 3 Induction of the ERβ binding affinity assays. Bars mean relative ERβ binding affinity level vs (-) control and present the mean ± SD. E2; 17β-estradiol, CWHL; Mixture of Cynanchi Wilfordii Radix and Humuli Lupuli Flos extract, HL; Humuli Lupuli Flos extract, CW; Cynanchi Wilfordii Radix extract. PN; 8-prenylnaringenin, CD; Caudatin. Statistical significance; (A) Each alphabet groups were significantly different ; p < 0.05. (B, C) * ; p < 0.05, ** ; p < 0.01, *** ; p < 0.001 vs (-) control.
그림 Fig. 4 The effect of CWHL on Inhibition of osteoclast differentiation in RANKLE induced osteoclastogenic RAW 264.7. CWHL; mixture of Cynanchi Wilfordii Radix and Humuli Lupuli Flos extract 30 μg/ml, HL; Humuli Lupuli Flos extract 10 μg/ml, CW; Cynanchi Wilfordii Radix extract 20 μg/ml, PN; 8-prenylnaringenin 20 ng/ml, CD; Caudatin 6 ng/ml, CDPN; mixture of caudatin and 8-prenylnaringenin 26 ng/ml. The osteoclast differentiation was observed through TRAP staining (Scale bar= 200 μm).

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