[논문]Isolation and characterization of two unrecorded yeast species in the phylum Basidiomycota

Jieun Seok; Jaewoo Bai; Sathiyaraj Srinivasan

doi:10.12651/jsr.2024.13.1.105

[국내논문] Isolation and characterization of two unrecorded yeast species in the phylum Basidiomycota

Journal of species research, v.13 no.1, 2024년, pp.105 - 110

Jieun Seok (College of Natural Science, Seoul Women's University) , Jaewoo Bai (College of Natural Science, Seoul Women's University) , Sathiyaraj Srinivasan (College of Natural Science, Seoul Women's University)

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The purpose of this study was to isolate and identify wild yeasts from soil samples collected in Seoul and Daejeon, Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and carbon source assimilation test were done using API 20C AUX kit. Among the 13 isolated strains, 11 strains were previously reported, but two strains have never been reported from Republic of Korea. The 13 strains were assigned to the phylum Basidiomycota. The two unrecorded yeast strains B2UV-201 and DJ1-5-B-10C belong to the genera Rhodotorula and Rhodosporidiobolus, respectively. The two unrecorded yeast strains are oval shaped and polar budding cells. This research focuses on the morphological and biochemical properties of the two unreported yeast species that have not officially been reported in Korea.

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그림 Fig. 1. Morphology of unrecorded strains incubated at 10℃. The colonies of Rhodotorula alborubescens B2UV-201 (A) and Rhodosporidiobolus odoratus DJ1-5-B-10C (B). Budding cells of Rhodotorula alborubescens B2UV-201 (C) and Rhodosporidiobolus odoratus DJ1-5-B-10C (D). All strains were grown for 3 days on YPD agar. Bars, 5 μm, and 10 μm, respectively.
표 Table 1. Yeasts isolated strains from soil in Republic of Korea. All strains were cultured at 10℃ for 3 days.
표 Table 2. Mycological characteristics of the unrecorded yeasts strains and closely related species.
그림 Fig. 2. A neighbor-joining phylogenetic tree constructed from a comparative analysis of 26S rRNA gene sequences showing the relationships of strain B2UV-201 with closely related species. Bootstrap values (>70%) are shown at the branch nodes. Bar, 0.01 substitutions per nucleotide position.
그림 Fig. 3. A neighbor-joining phylogenetic tree constructed from a comparative analysis of 26S rRNA gene sequences showing the relationships of strain DJ1-5-B-10C with closely related species. Bootstrap values (>70%) are shown at the branch nodes. Bar, 0.02 substitutions per nucleotide position.

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[국내논문] Isolation and characterization of two unrecorded yeast species in the phylum Basidiomycota

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[국내논문] Isolation and characterization of two unrecorded yeast species in the phylum Basidiomycota

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