[논문]배롱나무 꽃 추출물의 항여드름 화장품 소재로서의 특성 연구

유지영; 오세영; 임예지; 전수원; 노경백; 최송아; 조은애; 박덕훈; 정은선

doi:10.15230/scsk.2024.50.1.19

배롱나무 꽃 추출물의 항여드름 화장품 소재로서의 특성 연구
Study on the Properties of Lagerstroemia indica Extract as an Anti-acne Cosmetic Material 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.50 no.1, 2024년, pp.19 - 27

유지영 (바이오스펙트럼(주) 생명과학연구소) , 오세영 (바이오스펙트럼(주) 생명과학연구소) , 임예지 (바이오스펙트럼(주) 생명과학연구소) , 전수원 (바이오스펙트럼(주) 생명과학연구소) , 노경백 (바이오스펙트럼(주) 생명과학연구소) , 최송아 (바이오스펙트럼(주) 생명과학연구소) , 조은애 (바이오스펙트럼(주) 생명과학연구소) , 박덕훈 (바이오스펙트럼(주) 생명과학연구소) , 정은선 (바이오스펙트럼(주) 생명과학연구소)

초록
AI-Helper

본 연구는 배롱나무 꽃(Lagerstroemia indica flower)을 항여드름 화장품 소재로 제안하기 위한 것으로 배롱나무 꽃의 에탄올 추출물(Lagerstroemia indica flower extract, LIFE)을 이용해 피지조절과 항염, 항산화 효능을 확인하였다. 피지조절 효능을 평가한 결과 LIFE가 팔미트산으로 과도하게 유도된 피지생성을 65%까지 억제함을 확인하였다. 이어서 LIFE가 여드름 병변에서 주로 나타나는 Cutibacterium acnes (C. acnes)에 대한 항균작용과 lipase 활성억제능이 있으며, C. acnes로부터 분비되는 독성인자에 의한 염증반응도 감소시킬 수 있음을 확인하였다. 또한 LIFE가 대식세포에서 LPS로 유도된 염증성 매개인자인 일산화질소(NO)와 prostaglandin E₂ (PGE₂)의 분비를 각각 75%, 54%까지 억제함을 확인하였으며, 아스코르브산과 비슷한 정도의 높은 DPPH 라디칼 소거능 또한 있음을 확인하였다. 이 결과들은 천연 추출물인 LIFE가 피지 생성 조절과 함께, C. acnes에 대한 항균 및 lipase 활성 억제, 항염, 항산화능을 가짐으로써 복합성 질환인 여드름을 완화 및 치료하는 항여드름 소재로 활용될 수 있음을 제시한다.

Abstract ▼ AI-Helper

This study is to propose Lagerstroemia indica flower as an anti-acne cosmetic material, and confirmed the sebum control, anti-inflammatory, and antioxidant effects using Lagerstroemia indica flower extract (LIFE) . As a result of evaluating the sebum control effectiveness, it was confirmed that LIFE inhibited the production of sebum excessively induced with palmitic acid up to 65%. Subsequently, it was confirmed that LIFE has an antibacterial effect and the ability to inhibit lipase activity against Cutibacterium acnes (C. acnes), which mainly appears in acne lesions, and can also reduce the inflammatory response caused by virulence factors secreted by C. acnes. It was also confirmed that LIFE inhibited the secretion of nitrogen monoxide (NO) and prostaglandin E2 (PGE2), which are inflammatory mediators induced by LPS in macrophages, by 75% and 54%, respectively, and that it also had a high DPPH radical scavenging ability similar to that of ascorbic acid. These results suggest that LIFE, a natural extract, can be used as an anti-acne material to relieve and treat acne, a complex disease, by controlling sebum production and having antibacterial and lipase activity inhibiting against C. acnes, and anti-inflammatory, antioxidant properties.

주제어

표/그림 (5)

그림 Figure 1. Reduction of sebum production by LIFE in sebocytes. Sebocytes were incubated with various concentration (10, 50, and 100 μg/mL) LIFE under 100 μM palmitic acid for 48 h. Sebum production was confirmed by nile red staining, and fluorescence intensity of nile red was analyzed using ImageJ program. Scale bar is 100 μm. All results are shown as the mean ± SD of triplicate data. ^#p < 0.01 compared to control group, *p < 0.01, **p < 0.001 compared to palmitic acid treated group.
그림 Figure 2. Inhibition of C. acnes lipase activity and TLR2 activity by LIFE. (A) TLR2 activity was detected in HEK293-TLR2 cell after 6 h of treating the cells with LIFE and C. acnes cultured media (CM). (B) Cell viability under C. acnes CM and LIFE treated condition was measured by MTT assay. (C) C. acnes-derived lipase activity was detected using 4-Methylumbelliferyl oleate (4-MUO). Lipase was obtained by culturing C. acnes for 120 h and concentrating the supernatant collected by 20 times. After reacting C. acnes-derived lipase with 4-MUO for 18 h, absorbance was measured at 450 nm. All results are shown as the mean ± SD of triplicate data. *p < 0.01, **p < 0.001 compared to control group, ^#p < 0.01, ^##p < 0.001 compared to C. acnes CM-treated group.
표 Table 1. Minimum Inhibitory Concentration (MIC) of LIFE for C. acnes
그림 Figure 3. Supression of secretion of inflammatory mediators by LIFE. (A) RAW 264.7 cells were pre-treated with LIFE for 1 h and then incubated with LPS for 24 h. The level of NO production was determined by measuring nitrite, the major stable products of NO. (B) The secreted PGE₂ levels were detected using ELISA. All results are shown as the mean ± SD of triplicate data. ^#p < 0.001 compared to LPS untreated group, *p < 0.01, **p < 0.001 compared to LPS treated group.
그림 Figure 4. DPPH free radical scavenging of LIFE. DPPH was reacted with LIFE or 10 μg/mL ascorbic acid for 30 min and the radical scavenging capacity was determined by measuring absorbance at 517 nm. All results are shown as the mean ± SD of triplicate data. *p < 0.001 compared to control group.

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표제어: PCR

동의어: Packet Collision Rate

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