[논문]넓패추출물에 의한 RAW 264.7 세포에서의 항염효과

심중현

doi:10.15230/scsk.2024.50.1.29

넓패추출물에 의한 RAW 264.7 세포에서의 항염효과
Anti-inflammatory Effect of Ishige foliacea in RAW 264.7 Cells 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.50 no.1, 2024년, pp.29 - 36

심중현 (서울여자대학교 바이오헬스융합학과)

초록
AI-Helper

본 연구는 RAW 264.7 세포를 이용하여 넓패추출물(Ishige foliacea, I. foliacea)의 피부 항염 효과를 확인하고자 하였다. RAW 264.7 세포에 대한 넓패추출물의 항염증 효과는 세포 생존력 분석, mRNA 발현 및 산화질소(NO)/프로스타글란딘 E₂ (PGE₂) 생성을 통해 확인하였다. 넓패추출물의 항염증 효과는 IL-1α/IL-1β/IL-6/TNFα mRNA 발현 및 NO/PGE₂ 생성을 분석하여 규명하였고, 정량적 실시간 중합효소 연쇄반응을 통해 넓패추출물은 iNOS/COX2/IL-1α/IL-1β 및 IL-6의 mRNA 발현을 감소시키는 것을 확인하였다. 또한 PGE₂와 NO 생성의 비교를 통해 넓패추출물이 항염증 효과가 있음을 확인하였다. 넓패추출물의 항염 효과를 바탕으로 코스메슈티컬 소재로 활용될 수 있는 가능성을 확인하였다. 넓패추출물의 항염증 메커니즘에 대한 후속 연구를 바탕으로 코스메슈티컬뿐만 아니라 염증과 관련된 약리학적인 메커니즘 발굴에도 도움이 될 것으로 보인다.

Abstract ▼ AI-Helper

This study was carried out to identify the anti-inflammatory effects of Ishige foliacea (I. foliacea) extract on skin using RAW 264.7 cells. The anti-inflammatory effects of I. foliacea extract on RAW 264.7 cells were assessed by cell viability assay, mRNA expressions, and nitric oxide (NO)/prostaglandin E2 (PGE2) productions. The anti-inflammatory effects of I. foliacea extract were elucidated by analysis of IL-1α/IL-1β/IL-6/TNFα gene expressions and PGE2/NO production. Quantitative real-time polymerase chain reaction showed that I. foliacea extract decreased the gene expression levels of iNOS/COX2/IL-1α/IL-1β and IL-6. Furthermore, PGE2/NO production also revealed that I. foliacea extract exhibited anti-inflammatory properties. These results suggest that I. foliacea extract is an anti-inflammatory compound. It could be a potent cosmeceutical material for anti-inflammatory effects. Further studies on the anti-inflammatory mechanisms of broadleaf extracts are expected to help identify pharmacological mechanisms related to inflammation in addition to cosmeceuticals.

주제어

표/그림 (5)

그림 Figure 1. Cytotoxicity of Ishige foliacea (I. foliacea) extract in RAW 264.7 cells. RAW 264.7 cells (1 × 10⁴ cells/well) were seeded in 96 well cell culture plates and treated with I. foliacea extract for 24 h. The CCK-8 assay was used to perform the cell viability. These results are presented as mean ± SD of the percentage of control optical density in triplicate. * value relative to the control; *p < 0.05.
표 Table 1. Gene Name and Assay ID Number in Real-time RT-PCR Analysis
그림 Figure 2. Effects of I. foliacea extract on NO synthesis in RAW 264.7 cells. RAW 264 .7 c ells were seeded i n 35 mm cell c ulture d ishes and treated with I. foliacea extract for 24 h. (A) The conditioned media were analyzed for NO levels using a NO detection kit. (B) Realtime qPCR analysis of iNOS mRNA. The data represents the mean ± SD of three independent experiments. * value relative to the control; ^# value relative to the LPS-treated condition; *, ^# p < 0.05.
그림 Figure 3. Effects of I. foliacea extract on PGE2 production in RAW 264.7 cells. RAW 264.7 cells were seeded in 35 mm cell culture dishes and treated with I. foliacea extract for 24 h. (A) The conditioned media were analyzed for PGE₂ by PGE₂-ELISA . (B) Real-time qPCR analysis of COX2 mRNA. The data represents the mean ± SD of three independent experiments. * value relative to the control; ^# value relative to the LPS-treated condition; *, ^# p < 0.05.
그림 Figure 4. Effects of I. foliacea extract on the expression of inflammatory cytokines. Real-time qPCR analysis of inflammatory transcription factors was carried out for IL-1α (A), IL-1β (B), IL-6 (C), and TNFα (D). The graphs are shown as mean ± SD of three independent experiments. * value relative to the control; ^# value relative to the LPS-treated condition; *, ^# p < 0.05

참고문헌 (28)

J. Albina and J. Reichner, Nitric oxide in inflammation and immunity, New Horiz., 3(1), 46 (1995).？

상세보기
H. J. Kim and Y. C. Chang, Suppression of TNF-α-induced inflammation by extract from different parts of Moringa in HaCaT cells, J. Life Sci., 22(9), 1254 (2012).？
S. Akira and K. Takeda, Toll-like receptor signaling, Nat. Rev. Immunol., 4(7), 499 (2004).？
P. F. Gomez, M. H. Pilllinger, M. Attur, N. Marjanovic, M. Dave, J. Park, C. O. Binagham, H. Al-Mussawir, and S. B. Abramson, Resolution of inflammation:prostaglandin E 2 dissociates nuclear trafficking of individual NF-kappa B subunits (p65, p50) in stimulated rheumatoid synovial fibroblasts, J. Immunol., 175(10), 6924 (2005).？
C. Gabay, Interleukin-6 and chronic inflammation, Arthritis Res. Ther., 8(Suppl 2), S3 (2006).？
J. J. Triel, J. H. E. Arts, H. Muijser, and C. F. Kuper, Allergic inflammation in the upper respiratory tract of the rat upon repeated inhalation exposure to the contact allergen dinitrochlorobenzene (DNCB), Toxicol., 269(1), 73 (2010).？
H. J. Lee, B. Y. Sim, J. W. Bak, and D. H. Kim, Effect of Gami-sopungsan on inflammatory and DNCB-induced dermatitis in NC/Nga in mice, Korean J. Oriental Physiology & Pathology, 28(2), 146 (2014).？
N. J. Horwood, T. H. Page, J. P. McDaid, C. D. Palmer, J. Campbell, T. Mahon, F. M. Brennan, D. Webster, and B.M. Foxwell, Bruton's tyrosine kinase is required for TLR2 and TLR4-induced TNF, but not IL-6, production, J. Immunol., 176(6), 3635 (2006).？

상세보기
N. Hirohashi and D. C. Morrison, Low-dose lipopolysaccharide (LPS) pretreatment of mouse macrophages modulates LPS-dependent interleukin-6 production in vitro, Infect. Immun., 64(3), 1011 (1996).？

상세보기
C. H. Cho, M. Y. Kim, G. H. Youm, S. Kim, Y. K. Park, and S. H. Lee, Advanced glycation end-products inhibitory activities and renoprotective effects of Ishige foliacea ethanolic extract, J. Chitin Chitosan, 25(3), 134 (2020).？

상세보기
B. O. Cho, H. W. Ryu, Y. K. So, C. H. Jin, M. W. Byun, W. G. Kim, and I. Y. Jeong, Ishige sinicola extracts induce apoptosis via activation of a caspase cascade in human HeLa cell, J Korean Soc Food Sci Nutr, 41(7), 901 (2012).？
M. S. Kim, K. J. Kwon, M. J. Lee, S. M. Ahn, and H. Y. Sohn, Evaluation of the antimicrobial activities of 35 seaweed extracts against pathogenic bacteria and Candida sp, Korean J. Microbiol. Biotechnol., 40(2), 144 (2012).？
S. M. Ahn, Y. K. Hong, G. S. Kwon, and H. Y. Sohn, Evaluation of antioxidant and nitrite scavenging activity of seaweed extracts, Journal of Life Science, 21(4), 576 (2011).？
J. H. Lim, K. S. Jung, J. S. Lee, E. S. Jung, D. K. Kim, Y. S. Kim, Y. W. Kim, and D. H. Park, The study on antimicrobial and antifungal activity of the wild seaweeds of Jeju island, J. Soc. Cosmet. Sci. Korea, 34(3), 201 (2008).？
S. H. Cha, G. N. Ahn, S. J. Heo, K. N. Kim, K. W. Lee, C. B. Song, S. K. Cho, and Y.J. Jeon, Screening of extracts from marine green and brown algae in Jeju for potential marine angiotensin-I converting enzyme (ACE) inhibitory activity, J Korean Soc Food Sci Nutr, 35(3), 307 (2006).？
Ishige foliacea voucher CNUK PI046 cytochrome oxidase subunit III (cox3) gene, partial cds; mitochondrial. GenBank: FJ427587.1？
Ishige foliacea voucher CNUK PI046 ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; plastid. GenBank: FJ427692.1？
J. H. Kim, M. J. Kim, K. B. W. R. Kim, S. H. Pa, K. S. Cho, G. U Kim, X. Xiaotong, D. H. Lee, G. R. Park. and D.H. Ahn, Anti-inflammatory effects of Ishige sinicola ethanol extract in SLP-induced RAW 264.7 cell and mouse model, Korean J. Food Preserv., 24(8), 1149 (2017).？
A. N. Paunel, A. Dejam, S. Thelen, M. Kirsch, M. Horstjann, P. Gharini, M. Murtz, M. Kelm, H. de Groot, V. Kolb-Bachofen, and C. V Suschek, Enzyme-independent nitric oxide formation during UVA challenge of human skin: characterization, molecular sources, and mechanisms, Free Radic. Biol. Med., 38(5), 606 (2005).？

상세보기
K. J. Yun, J. Y. Kim, J. B. Kim, K. W. Lee, S. Y. Jeong, H. J. Park, H. J. Jung, Y. W. Cho, K. Yun, and K. T. Lee, Inhibition of LPS-induced NO and PGE 2 production by asiatic acid via NF-kB inactivation in RAW 264.7 macrophages: Possible involvement of the IKK and MAPK pathways, Internation. Immunopharmacol., 8(3), 431 (2008).？
S. G. Harris, J. Padilla, L. Koumas, D. Ray, and R. P. Phipps, Prostaglandins as modulators of immunity, Trends Immunol., 23(3), 144 (2002).？

상세보기
A. S. M. Noman, N. Koide, F. Hassan, I. I-E-Khuda, J. Dagvadorj, G. Tumurkhuu, S. Islam, Y. Naiki, T. Yoshida, and T. Yokochi, Thalidomide inhibits lipopolysaccharide-induced tumor necrosis factor-a production via downregulation of MyD88 expression, Innate Immun., 15(1), 33 (2009).？
P. C. Lord, L. M. Wilmoth, S. B. Mizel, and C.E. McCall, Expression of interleukin-1 alpha and beta genes by human blood polymorphonuclear leukocytes, J. Clin Invest., 87(4), 1312 (1991).？

상세보기
I. Roitt, J. Brostoff and D. Male, Immunology sixth edition, p119,128,441, Mosby, London, U.K. (2002).？
Y. Zhang, B. F. Ramos, and B. A. Jakschik, Neutrophil recruitment by tumor necrosis factor from mast cells in immune complex peritonitis, Science, 258(5090), 1957 (1992).？

상세보기
H. S. Talwar, C. E. Griffiths, G. J. Fisher, T. A. Hamilton, and J. J. Voorhees, Reduced type I and type III procollagens in photodamaged adult human skin, J. Invest. Dermatol., 105(2), 285 (1995).？

상세보기
J. Kim, C. W. Lee, E. K. Kim, S. J. Lee, N. H. Park, H. S. Kim, H. K. Kim, K. Char, Y. P. Jang, and J. W. Kim, Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts, J. Enthnopharmacol., 137(1), 427 (2011).？
T. B. L. Kirkwood, Understanding the odd science of aging, Cell, 120(4), 437 (2005).

상세보기

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