[논문]매실나무 가지 추출물의 항산화, 항염, 항균 활성 및 유효성분 연구

김혜빈; 김정은; 이남호

doi:10.15230/scsk.2024.50.3.227

[국내논문] 매실나무 가지 추출물의 항산화, 항염, 항균 활성 및 유효성분 연구
Anti-oxidative, Anti-inflammatory and Anti-bacterial Constituents from the Extract of Prunus mume Branches

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.50 no.3, 2024년, pp.227 - 237

김혜빈 (제주대학교 화학.코스메틱스학과) , 김정은 (제주대학교 화학.코스메틱스학과) , 이남호 (제주대학교 화학.코스메틱스학과)

초록
AI-Helper

본 연구에서는 매실나무(Prunus mume, P. mume) 가지 추출물의 항산화, 항염, 항균 활성 및 유효성분을 연구하였다. 매실나무 가지의 ethyl acetate (EtOAc) 분획물에서 noreugenin (1), naringenin (2), prunin (3), procyanidin A2 (4) 및 epiafzelechin-(2β→O→7, 4β→8)-epicatechin (5)의 5개 화합물을 분리하여 NMR 스펙트럼을 포함한 분광 데이터를 기반으로 화학 구조를 동정하였다. DPPH 및 ABTS⁺ 라디칼을 이용한 항산화 실험에서 추출물, EtOAc 및 n-butanol (BuOH) 분획물과 분리된 화합물 4, 5는 강력한 라디칼 소거 활성을 보였다. RAW 264.7 대식세포를 이용한 항염 활성 실험 결과, n-hexane (Hex), EtOAc 분획물 및 분리된 화합물 1 ~ 5는 세포 독성 없이 농도 의존적으로 nitric oxide (NO)의 생성을 억제하였다. 또한, 추출물과 n-Hex, EtOAc 및 n-BuOH 분획물은 Staphylococcus epidermidis 및 Cutibacterium acnes에 대하여 항균 활성을 보였다. 이상의 연구 결과를 바탕으로 매실나무 가지 추출물, 용매 분획물 및 분리된 화합물은 항산화, 항염 및 항균 효과를 갖는 천연 화장품 소재로 적용 가능할 것이라 사료된다.

Abstract ▼ AI-Helper

In this study, we investigated anti-oxidative, anti-inflammatory and anti-bacterial constituents from the extract of Prunus mume (P. mume) branches. Five phytochemicals were isolated from the ethyl acetate (EtOAc) fraction of P. mume branches; noreugenin (1), naringenin (2), prunin (3), procyanidin A2 (4) and epiafzelechin-(2β→O→7, 4β→8)-epicatechin (5). The chemical structures of isolated compounds were elucidated based on the spectroscopic data including NMR spectra as well as comparison of the data to the literature values. Upon the anti-oxidative studies by DPPH and ABTS+ radicals, potent radical scavenging activities were observed in the extract, EtOAc, n-butanol (BuOH) fractions and isolated compounds 4, 5. In the anti-inflammatory tests using RAW 264.7 macrophages, the n-hexane (Hex), EtOAc fractions and compounds 1-5 inhibited production of nitric oxide (NO) without causing cell toxicity. Also, the extract, n-Hex, EtOAc and n-BuOH fractions showed anti-bacterial activities against Staphylococcus epidermidis and Cutibacterium acnes. Based on these results, it was suggested that the extract, solvent fractions and phytochemicals from P. mume branches could be applicable as natural source for cosmetic ingredients.

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표/그림 (10)

$Figure 1. DPPH (A) and ABTS<sup>+</sup> (B) radical scavenging activities of extract and solvent fractions from Prunus mume (P. mume) branches. The data are expressed as a percentage of control and represent the mean ± SD of triplicate experiments.$ 그림 Figure 1. DPPH (A) and ABTS⁺ (B) radical scavenging activities of extract and solvent fractions from Prunus mume (P. mume) branches. The data are expressed as a percentage of control and represent the mean ± SD of triplicate experiments.
표 Table 1. SC₅₀ Values of DPPH and ABTS⁺ Radical Scavenging Activities of Extract and Solvent Fractions from P. mume Branches
$Figure 2. Effects of extract and solvent fractions from P. mume branches on NO production and cell viability in LPS-stimulated RAW 264.7 cells. The cells were stimulated with 100 ng/mL of LPS only, or with LPS plus extract and solvent fractions from P. mume branches for 24 h. The data represent the mean ± SD of triplicate experiments. (* p < 0.05, **p < 0.01)$ 그림 Figure 2. Effects of extract and solvent fractions from P. mume branches on NO production and cell viability in LPS-stimulated RAW 264.7 cells. The cells were stimulated with 100 ng/mL of LPS only, or with LPS plus extract and solvent fractions from P. mume branches for 24 h. The data represent the mean ± SD of triplicate experiments. (* p < 0.05, **p < 0.01)
$Figure 3. E ffects of n-Hex (A) and EtOAc (B) fractions from P. mume branches on NO production and cell viability in LPS-stimulated RAW 264.7 cells. The cells were stimulated with 100 ng/mL of LPS only, or with LPS plus n-Hex and EtOAc fractions from P. mume branches for 24 h. The data represent the mean ± SD of triplicate experiments. ( * p < 0.05, **p < 0.01)$ 그림 Figure 3. E ffects of n-Hex (A) and EtOAc (B) fractions from P. mume branches on NO production and cell viability in LPS-stimulated RAW 264.7 cells. The cells were stimulated with 100 ng/mL of LPS only, or with LPS plus n-Hex and EtOAc fractions from P. mume branches for 24 h. The data represent the mean ± SD of triplicate experiments. ( * p < 0.05, **p < 0.01)
그림 Figure 4. Chemical structures of isolated compounds 1-5 from P. mume branches.
표 Table 2. Anti-bacterial Activities of Extract and Solvent Fractions from P. mume Branches
그림 Figure 5. DPPH (A) and ABTS⁺ (B) radical scavenging activities of isolated compounds 1-5 from P. mume branches. The data are expressed as a percentage of control and represent the mean ± SD of triplicate experiments.
표 Table 3. SC₅₀ Values of DPPH and ABTS⁺ Radical Scavenging Activities of Isolated Compounds 1-5 from P. mume Branches
그림 Figure 7. HPLC chromatogram of 70% EtOH extract from P. mume branches at 280 nm.
그림 Figure 6. Effects of isolated compounds 1-5 from P. mume branches on NO production and cell viability in LPS-stimulated RAW 264.7 cells. The cells were stimulated with 100 ng/mL of LPS only, or with LPS plus compounds 1-5 from P. mume branches for 24 h. The data represent the mean ± SD of triplicate experiments. (* p < 0.05, **p < 0.01)

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