[논문]Type 2 Diabetes Alters Intracellular Ca 2+ Handling in Native Endothelium of Excised Rat Aorta

Berra-Romani, Roberto; Guzmá; n-Silva, Alejandro; Vargaz-Guadarrama, Ajelet; Flores-Alonso, Juan Carlos; Alonso-Romero, José; Treviñ; o, Samuel; Sá; nchez-Gó; mez, Josué; Coyotl-Santiago, Nayeli; Garcí; a-Carrasco, Mario; Moccia, Francesco

doi:10.3390/ijms21010250

Type 2 Diabetes Alters Intracellular Ca 2+ Handling in Native Endothelium of Excised Rat Aorta 원문보기

International journal of molecular sciences, v.21 no.1, 2020년, pp.250 -

Berra-Romani, Roberto (Laboratory of Cardiovascular Physiology, Biomedicine School, Faculty of Medicine, Benemé) , Guzmán-Silva, Alejandro (rita Universidad Autó) , Vargaz-Guadarrama, Ajelet (noma de Puebla, Puebla 72410, Mexico) , Flores-Alonso, Juan Carlos (aleckz.guzman@gmail.com (A.G.-S.)) , Alonso-Romero, José (oublitte_15@hotmail.com (J.A.-R.)) , Treviño, Samuel (nayevader@gmail.com (N.C.-S.)) , Sánchez-Gómez, Josué (Laboratory of Cardiovascular Physiology, Biomedicine School, Faculty of Medicine, Benemé) , Coyotl-Santiago, Nayeli (rita Universidad Autó) , García-Carrasco, Mario (noma de Puebla, Puebla 72410, Mexico) , Moccia, Francesco (aleckz.guzman@gmail.com (A.G.-S.))

Abstract ▼ AI-Helper

An increase in intracellular Ca2+ concentration ([Ca2+]i) plays a key role in controlling endothelial functions; however, it is still unclear whether endothelial Ca2+ handling is altered by type 2 diabetes mellitus, which results in severe endothelial dysfunction. Herein, we analyzed for the first time the Ca2+ response to the physiological autacoid ATP in native aortic endothelium of obese Zucker diabetic fatty (OZDF) rats and their lean controls, which are termed LZDF rats. By loading the endothelial monolayer with the Ca2+-sensitive fluorophore, Fura-2/AM, we found that the endothelial Ca2+ response to 20 µM and 300 µM ATP exhibited a higher plateau, a larger area under the curve and prolonged duration in OZDF rats. The “Ca2+ add-back” protocol revealed no difference in the inositol-1,4,5-trisphosphate-releasable endoplasmic reticulum (ER) Ca2+ pool, while store-operated Ca2+ entry was surprisingly down-regulated in OZDF aortae. Pharmacological manipulation disclosed that sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) activity was down-regulated by reactive oxygen species in native aortic endothelium of OZDF rats, thereby exaggerating the Ca2+ response to high agonist concentrations. These findings shed new light on the mechanisms by which type 2 diabetes mellitus may cause endothelial dysfunction by remodeling the intracellular Ca2+ toolkit.

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81. Petersen O.H. Tepikin A.V. Gerasimenko J.V. Gerasimenko O.V. Sutton R. Criddle D.N. Fatty acids, alcohol and fatty acid ethyl esters: Toxic Ca2+ signal generation and pancreatitis Cell Calcium 2009 45 634 642 10.1016/j.ceca.2009.02.005 19327825

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82. Trevino S. Waalkes M.P. Flores Hernandez J.A. Leon-Chavez B.A. Aguilar-Alonso P. Brambila E. Chronic cadmium exposure in rats produces pancreatic impairment and insulin resistance in multiple peripheral tissues Arch. Biochem. Biophys. 2015 583 27 35 10.1016/j.abb.2015.07.010 26253262

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83. Berra-Romani R. Raqeeb A. Torres-Jacome J. Guzman-Silva A. Guerra G. Tanzi F. Moccia F. The Mechanism of Injury-Induced Intracellular Calcium Concentration Oscillations in the Endothelium of Excised Rat Aorta J. Vasc. Res. 2012 49 65 76 10.1159/000329618 21997119

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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Type 2 Diabetes Alters Intracellular Ca 2+ Handling in Native Endothelium of Excised Rat Aorta 원문보기

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