[논문]Purinergic Signaling Pathway in Human Olfactory Neuronal Precursor Cells

Solí; s-Chagoyá; n, Hé; ctor; Flores-Soto, Edgar; Valdé; s-Tovar, Marcela; Cercó; s, Montserrat G.; Calixto, Eduardo; Montañ; o, Luis M.; Barajas-Ló; pez, Carlos; Sommer, Bettina; Aquino-Gá; lvez, Arnoldo; Trueta, Citlali; Bení; tez-King, Gloria A.

doi:10.1155/2019/2728786

Purinergic Signaling Pathway in Human Olfactory Neuronal Precursor Cells 원문보기

Stem cells international, v.2019, 2019년, pp.2728786 -

Solís-Chagoyán, Héctor (Instituto Nacional de Psiquiatrí) , Flores-Soto, Edgar (a Ramó) , Valdés-Tovar, Marcela (n de la Fuente Muñ) , Cercós, Montserrat G. (iz, Laboratorio de Neurofarmacologí) , Calixto, Eduardo (a, Calzada Mé) , Montaño, Luis M. (xico-Xochimilco 101, San Lorenzo Huipulco, CP 14370 Ciudad de Mé) , Barajas-López, Carlos (xico, Mexico) , Sommer, Bettina (Universidad Nacional Autó) , Aquino-Gálvez, Arnoldo (noma de Mé) , Trueta, Citlali (xico, Departamento de Farmacologí) , Benítez-King, Gloria A. (a, Facultad de Medicina, CP 04510 Ciudad de Mé)

Abstract ▼ AI-Helper

Extracellular ATP and trophic factors released by exocytosis modulate in vivo proliferation, migration, and differentiation in multipotent stem cells (MpSC); however, the purinoceptors mediating this signaling remain uncharacterized in stem cells derived from the human olfactory epithelium (hOE). Our aim was to determine the purinergic pathway in isolated human olfactory neuronal precursor cells (hONPC) that exhibit MpSC features. Cloning by limiting dilution from a hOE heterogeneous primary culture was performed to obtain a culture predominantly constituted by hONPC. Effectiveness of cloning to isolate MpSC-like precursors was corroborated through immunodetection of specific protein markers and by functional criteria such as self-renewal, proliferation capability, and excitability of differentiated progeny. P2 receptor expression in hONPC was determined by Western blot, and the role of these purinoceptors in the ATP-induced exocytosis and changes in cytosolic Ca2+ ([Ca2+]i) were evaluated using the fluorescent indicators FM1-43 and Fura-2 AM, respectively. The clonal culture was enriched with SOX2 and OCT3/4 transcription factors; additionally, the proportion of nestin-immunopositive cells, the proliferation capability, and functionality of differentiated progeny remained unaltered through the long-term clonal culture. hONPC expressed P2X receptor subtypes 1, 3-5, and 7, as well as P2Y2, 4, 6, and 11; ATP induced both exocytosis and a transient [Ca2+]i increase predominantly by activation of metabotropic P2Y receptors. Results demonstrated for the first time that ex vivo-expressed functional P2 receptors in MpSC-like hONPC regulate exocytosis and Ca2+ signaling. This purinergic-triggered release of biochemical messengers to the extracellular milieu might be involved in the paracrine signaling among hOE cells.

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표제어: PCR

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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Purinergic Signaling Pathway in Human Olfactory Neuronal Precursor Cells 원문보기

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