[논문]Spatial and temporal diversity of glycome expression in mammalian brain

Lee, Jua; Ha, Seungshin; Kim, Minsoo; Kim, Seong-Wook; Yun, Jaekyung; Ozcan, Sureyya; Hwang, Heeyoun; Ji, In Jung; Yin, Dongtan; Webster, Maree J.; Shannon Weickert, Cynthia; Kim, Jae-Han; Yoo, Jong Shin; Grimm, Rudolf; Bahn, Sabine; Shin, Hee-Sup; An, Hyun Joo

doi:10.1073/pnas.2014207117

[해외논문] Spatial and temporal diversity of glycome expression in mammalian brain 원문보기

Proceedings of the National Academy of Sciences of the United States of America, v.117 no.46, 2020년, pp.28743 - 28753

Lee, Jua (Graduate School of Analytical Science & Technology, Chungnam National University, 34134 Daejeon, South Korea) , Ha, Seungshin , Kim, Minsoo (Center for Cognition and Sociality, Institute for Basic Science, 34051 Daejeon, South Korea) , Kim, Seong-Wook , Yun, Jaekyung (Center for Cognition and Sociality, Institute for Basic Science, 34051 Daejeon, South Korea) , Ozcan, Sureyya , Hwang, Heeyoun (Center for Cognition and Sociality, Institute for Basic Science, 34051 Daejeon, South Korea) , Ji, In Jung , Yin, Dongtan (Graduate School of Analytical Science & Technology, Chungnam National University, 34134 Daejeon, South Korea) , Webster, Maree J. , Shannon Weickert, Cynthia (Department of Chemical Engineering and Biotechnology, University of Cambridge, CB2 1QT Cambridge, United Kingdom) , Kim, Jae-Han , Yoo, Jong Shin (Research Center for Bioconvergence Analysis, Korea Basic Science Institute, 28119 Cheongju, South Korea) , Grimm, Rudolf , Bahn, Sabine (Graduate School of Analytical Science & Technology, Chungnam National University, 34134 Daejeon, South Korea) , Shin, Hee-Sup , An, Hyun Joo (Graduate School of Analytical Science & Technology, Chungnam National University, 34134 Daejeon, South Korea)

Abstract ▼ AI-Helper

SignificanceDeciphering molecular mechanisms at the glycome level in mammalian brain remains a missing piece of the puzzle in molecular neuroscience due to the intrinsic complexity of glycosylation and the lack of analytical tools. Here, we uncovered the variation and diversity of glycome expression in human and mouse brain samples according to spatial and temporal differences. We further constructed a comprehensive synthesis map using glycans structurally elucidated by LC-MS/MS and found strong evidence on the conservation and developmental divergence of human and mouse prefrontal cortex N-glycome. Our data could be the reference for future mammalian brain glycome study. Furthermore, it provides valuable information on human brain glycome, which has languished in relative obscurity.Mammalian brain glycome remains a relatively poorly understood area compared to other large-scale “omics” studies, such as genomics and transcriptomics due to the inherent complexity and heterogeneity of glycan structure and properties. Here, we first performed spatial and temporal analysis of glycome expression patterns in the mammalian brain using a cutting-edge experimental tool based on liquid chromatography-mass spectrometry, with the ultimate aim to yield valuable implications on molecular events regarding brain functions and development. We observed an apparent diversity in the glycome expression patterns, which is spatially well-preserved among nine different brain regions in mouse. Next, we explored whether the glycome expression pattern changes temporally during postnatal brain development by examining the prefrontal cortex (PFC) at different time point across six postnatal stages in mouse. We found that glycan expression profiles were dynamically regulated during postnatal developments. A similar result was obtained in PFC samples from humans ranging in age from 39 d to 49 y. Novel glycans unique to the brain were also identified. Interestingly, changes primarily attributed to sialylated and fucosylated glycans were extensively observed during PFC development. Finally, based on the vast heterogeneity of glycans, we constructed a core glyco-synthesis map to delineate the glycosylation pathway responsible for the glycan diversity during the PFC development. Our findings reveal high levels of diversity in a glycosylation program underlying brain region specificity and age dependency, and may lead to new studies exploring the role of glycans in spatiotemporally diverse brain functions.

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참고문헌 (51)

Hawrylycz, Michael J., Lein, Ed S., Guillozet-Bongaarts, Angela L., Shen, Elaine H., Ng, Lydia, Miller, Jeremy A., van de Lagemaat, Louie N., Smith, Kimberly A., Ebbert, Amanda, Riley, Zackery L., Abajian, Chris, Beckmann, Christian F., Bernard, Amy, Bertagnolli, Darren, Boe, Andrew F., Cartagena, Preston M., Chakravarty, M. Mallar, Chapin, Mike, Chong, Jimmy, Dalley, Rachel A., Daly, Barry David, Dang, Chinh, Datta, Suvro, Dee, Nick, Dolbeare, Tim A., Faber, Vance, Feng, David, Fowler, David R., Goldy, Jeff, Gregor, Benjamin W., Haradon, Zeb, Haynor, David R., Hohmann, John G., Horvath, Steve, Howard, Robert E., Jeromin, Andreas, Jochim, Jayson M., Kinnunen, Marty, Lau, Christopher, Lazarz, Evan T., Lee, Changkyu, Lemon, Tracy A., Li, Ling, Li, Yang, Morris, John A., Overly, Caroline C., Parker, Patrick D., Parry, Sheana E., Reding, Melissa, Royall, Joshua J.. An anatomically comprehensive atlas of the adult human brain transcriptome. Nature, vol.489, no.7416, 391-399.

상세보기
Ivanisevic, J., Epstein, Adrian A., Kurczy, Michael E., Benton, Paul H., Uritboonthai, W., Fox, Howard S., Boska, Michael D., Gendelman, Howard E., Siuzdak, G.. Brain Region Mapping Using Global Metabolomics. Chemistry & biology, vol.21, no.11, 1575-1584.

상세보기
Ng, Lydia, Bernard, Amy, Lau, Chris, Overly, Caroline C, Dong, Hong-Wei, Kuan, Chihchau, Pathak, Sayan, Sunkin, Susan M, Dang, Chinh, Bohland, Jason W, Bokil, Hemant, Mitra, Partha P, Puelles, Luis, Hohmann, John, Anderson, David J, Lein, Ed S, Jones, Allan R, Hawrylycz, Michael. An anatomic gene expression atlas of the adult mouse brain. Nature neuroscience, vol.12, no.3, 356-362.

상세보기
Sharma, Kirti, Schmitt, Sebastian, Bergner, Caroline G, Tyanova, Stefka, Kannaiyan, Nirmal, Manrique-Hoyos, Natalia, Kongi, Karina, Cantuti, Ludovico, Hanisch, Uwe-Karsten, Philips, Mari-Anne, Rossner, Moritz J, Mann, Matthias, Simons, Mikael. Cell type– and brain region–resolved mouse brain proteome. Nature neuroscience, vol.18, no.12, 1819-1831.

상세보기
Lein, Ed S., Hawrylycz, Michael J., Ao, Nancy, Ayres, Mikael, Bensinger, Amy, Bernard, Amy, Boe, Andrew F., Boguski, Mark S., Brockway, Kevin S., Byrnes, Emi J., Chen, Lin, Chen, Li, Chen, Tsuey-Ming, Chi Chin, Mei, Chong, Jimmy, Crook, Brian E., Czaplinska, Aneta, Dang, Chinh N., Datta, Suvro, Dee, Nick R., Desaki, Aimee L., Desta, Tsega, Diep, Ellen, Dolbeare, Tim A., Donelan, Matthew J., Dong, Hong-Wei, Dougherty, Jennifer G., Duncan, Ben J., Ebbert, Amanda J., Eichele, Gregor, Estin, Lili K., Faber, Casey, Facer, Benjamin A., Fields, Rick, Fischer, Shanna R., Fliss, Tim P., Frensley, Cliff, Gates, Sabrina N., Glattfelder, Katie J., Halverson, Kevin R., Hart, Matthew R., Hohmann, John G., Howell, Maureen P., Jeung, Darren P., Johnson, Rebecca A., Karr, Patrick T., Kawal, Reena, Kidney, Jolene M., Knapik, Rachel H., Kuan, Chihchau L.. Genome-wide atlas of gene expression in the adult mouse brain. Nature, vol.445, no.7124, 168-176.

상세보기
Bakken, Trygve E., Miller, Jeremy A., Ding, Song-Lin, Sunkin, Susan M., Smith, Kimberly A., Ng, Lydia, Szafer, Aaron, Dalley, Rachel A., Royall, Joshua J., Lemon, Tracy, Shapouri, Sheila, Aiona, Kaylynn, Arnold, James, Bennett, Jeffrey L., Bertagnolli, Darren, Bickley, Kristopher, Boe, Andrew, Brouner, Krissy, Butler, Stephanie, Byrnes, Emi, Caldejon, Shiella, Carey, Anita, Cate, Shelby, Chapin, Mike, Chen, Jefferey, Dee, Nick, Desta, Tsega, Dolbeare, Tim A., Dotson, Nadia, Ebbert, Amanda, Fulfs, Erich, Gee, Garrett, Gilbert, Terri L., Goldy, Jeff, Gourley, Lindsey, Gregor, Ben, Gu, Guangyu, Hall, Jon, Haradon, Zeb, Haynor, David R., Hejazinia, Nika, Hoerder-Suabedissen, Anna, Howard, Robert, Jochim, Jay, Kinnunen, Marty, Kriedberg, Ali, Kuan, Chihchau L., Lau, Christopher, Lee, Chang-Kyu, Lee, Felix, Luong, Lon, Mastan, Naveed, May, Ryan, Melchor, Jose, Mosqueda, Nerick, Mott, Erika, Ngo, Kiet, Nyhus, Julie, Oldre, Aaron, Olso. Comprehensive transcriptional map of primate brain development. Nature, vol.535, no.7612, 367-375.

상세보기
Frese, Christian K., Mikhaylova, Marina, Stucchi, Riccardo, Gautier, Violette, Liu, Qingyang, Mohammed, Shabaz, Heck, Albert J.R., Altelaar, A.F. Maarten, Hoogenraad, Casper C.. Quantitative Map of Proteome Dynamics during Neuronal Differentiation. Cell reports, vol.18, no.6, 1527-1542.

상세보기
Jové, Mariona, Portero-Otín, Manuel, Naudí, Alba, Ferrer, Isidre, Pamplona, Reinald. Metabolomics of Human Brain Aging and Age-Related Neurodegenerative Diseases. Journal of neuropathology and experimental neurology, vol.73, no.7, 640-657.

상세보기
Ohtsubo, Kazuaki, Marth, Jamey D.. Glycosylation in Cellular Mechanisms of Health and Disease. Cell, vol.126, no.5, 855-867.

상세보기
10.1007/978-1-4613-2925-1_3
Yamakawa, Nao, Vanbeselaere, Jorick, Chang, Lan-Yi, Yu, Shin-Yi, Ducrocq, Lucie, Harduin-Lepers, Anne, Kurata, Junichi, Aoki-Kinoshita, Kiyoko F., Sato, Chihiro, Khoo, Kay-Hooi, Kitajima, Ken, Guerardel, Yann. Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns. Nature communications, vol.9, no.1, 4647-.

상세보기
Kleene, Ralf, Schachner, Melitta. Glycans and neural cell interactions. Nature reviews. Neuroscience, vol.5, no.3, 195-208.

상세보기
Hakomori, S. Glycosphingolipids in Cellular Interaction, Differentiation, and Oncogenesis. Annual review of biochemistry, vol.50, 733-764.

상세보기
Lau, Ken S., Partridge, Emily A., Grigorian, Ani, Silvescu, Cristina I., Reinhold, Vernon N., Demetriou, Michael, Dennis, James W.. Complex N-Glycan Number and Degree of Branching Cooperate to Regulate Cell Proliferation and Differentiation. Cell, vol.129, no.1, 123-134.

상세보기
Zoldos, V., Horvat, T., Lauc, G.. Glycomics meets genomics, epigenomics and other high throughput omics for system biology studies. Current opinion in chemical biology, vol.17, no.1, 34-40.

상세보기
Transforming Glycoscience: A Roadmap for the Future National Research Council 2012 National Research Council, Transforming Glycoscience: A Roadmap for the Future (National Academies Press, 2012).
Zielinska, Dorota F., Gnad, F., Schropp, K., Wisniewski, Jacek R., Mann, M.. Mapping N-Glycosylation Sites across Seven Evolutionarily Distant Species Reveals a Divergent Substrate Proteome Despite a Common Core Machinery. Molecular cell, vol.46, no.4, 542-548.

상세보기
Essentials of Glycobiology Cummings R. 2017 3 R. Cummings, F. Liu, G. Vasta, “Chapter 1: Historical Background and Overview” in Essentials of Glycobiology, A. Varki, Ed. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 3rd Ed., 2017).
Rudd, Pauline M., Merry, Anthony H., Wormald, Mark R., Dwek, Raymond A.. Glycosylation and prion protein. Current opinion in structural biology, vol.12, no.5, 578-586.

상세보기
Gao, Wei-Na, Yau, Lee-Fong, Liu, Liang, Zeng, Xing, Chen, Da-Can, Jiang, Min, Liu, Ju, Wang, Jing-Rong, Jiang, Zhi-Hong. Microfluidic Chip-LC/MS-based Glycomic Analysis Revealed Distinct N -glycan Profile of Rat Serum. Scientific reports, vol.5, 12844-.

상세보기
Wu, Di, Struwe, Weston B., Harvey, David J., Ferguson, Michael A. J., Robinson, Carol V.. N-glycan microheterogeneity regulates interactions of plasma proteins. Proceedings of the National Academy of Sciences of the United States of America, vol.115, no.35, 8763-8768.

상세보기
Ji, In Jung, Hua, Serenus, Shin, Dong Hee, Seo, Nari, Hwang, Jae Yun, Jang, Ik-Soon, Kang, Myoung-Goo, Choi, Jong-Soon, An, Hyun Joo. Spatially-Resolved Exploration of the Mouse Brain Glycome by Tissue Glyco-Capture (TGC) and Nano-LC/MS. Analytical chemistry, vol.87, no.5, 2869-2877.

상세보기
10.1093/acprof:oso/9780195134971.003.0029
Fuster, Joaquín M.. Frontal lobe and cognitive development. Journal of neurocytology, vol.31, no.3, 373-385.

상세보기
Semple, B.D., Blomgren, K., Gimlin, K., Ferriero, D.M., Noble-Haeusslein, L.J.. Brain development in rodents and humans: Identifying benchmarks of maturation and vulnerability to injury across species. Progress in neurobiology, vol.106, 1-16.

상세보기
Gotoh, Masanori, Sato, Takashi, Kiyohara, Katsue, Kameyama, Akihiko, Kikuchi, Norihiro, Kwon, Yeon-Dae, Ishizuka, Yasuko, Iwai, Toshie, Nakanishi, Hiroshi, Narimatsu, Hisashi. Molecular cloning and characterization of β1,4-N-acetylgalactosaminyltransferases IV synthesizing N,N′-diacetyllactosediamine. FEBS letters, vol.562, no.1, 134-140.

상세보기
Machado, E., Kandzia, S., Carilho, R., Altevogt, P., Conradt, H. S., Costa, J.. N-Glycosylation of total cellular glycoproteins from the human ovarian carcinoma SKOV3 cell line and of recombinantly expressed human erythropoietin. Glycobiology, vol.21, no.3, 376-386.

상세보기
Fukushima, K., Satoh, T., Baba, S., Yamashita, K.. 1,2-Fucosylated and -N-acetylgalactosaminylated prostate-specific antigen as an efficient marker of prostatic cancer. Glycobiology, vol.20, no.4, 452-460.

상세보기
Fiete, D., Srivastava, V., Hindsgaul, O., Baenziger, J.U.. A hepatic reticuloendothelial cell receptor specific for SO₄-4GalNAcβ1, 4GlcNAcβ1,2Manα that mediates rapid clearance of lutropin. Cell, vol.67, no.6, 1103-1110.

상세보기
Sasaki, Norihiko, Shinomi, Masahito, Hirano, Kazumi, Ui-Tei, Kumiko, Nishihara, Shoko. LacdiNAc (GalNAcβ1-4GlcNAc) Contributes to Self-Renewal of Mouse Embryonic Stem Cells by Regulating Leukemia Inhibitory Factor/STAT3 Signaling. Stem cells^®, vol.29, no.4, 641-650.

상세보기
Sato, T., Taka, J., Aoki, N., Matsuda, T., Furukawa, K.. Expression of -N-Acetylgalactosaminylated N-Linked Sugar Chains Is Associated with Functional Differentiation of Bovine Mammary Gland. The Journal of biochemistry, vol.122, no.5, 1068-1073.

상세보기
Glycoconj. J. Torii T. 671 31 2014 Determination of major sialylated N-glycans and identification of branched sialylated N-glycans that dynamically change their content during development in the mouse cerebral cortex T. Torii ., Determination of major sialylated N-glycans and identification of branched sialylated N-glycans that dynamically change their content during development in the mouse cerebral cortex. Glycoconj. J. 31, 671-683 (2014).
Saghatelyan, Armen K., Gorissen, Silke, Albert, Martine, Hertlein, Birgit, Schachner, Melitta, Dityatev, Alexander. The extracellular matrix molecule tenascin‐R and its HNK‐1 carbohydrate modulate perisomatic inhibition and long‐term potentiation in the CA1 region of the hippocampus. The European journal of neuroscience, vol.12, no.9, 3331-3342.

상세보기
Chou, Denise K. H., Evans, James E., Jungalwala, Firoze B.. Identity of nuclear high-mobility-group protein, HMG-1, and sulfoglucuronyl carbohydrate-binding protein, SBP-1, in brain. Journal of neurochemistry, vol.77, no.1, 120-131.

상세보기
Yamamoto, Shoji, Oka, Shogo, Inoue, Mitsuhiro, Shimuta, Misa, Manabe, Toshiya, Takahashi, Hideki, Miyamoto, Masaomi, Asano, Masahide, Sakagami, Junko, Sudo, Katsuko, Iwakura, Yoichiro, Ono, Katsuhiko, Kawasaki, Toshisuke. Mice Deficient in Nervous System-specific Carbohydrate Epitope HNK-1 Exhibit Impaired Synaptic Plasticity and Spatial Learning. The Journal of biological chemistry, vol.277, no.30, 27227-27231.

상세보기
Belmonte, J., Callaway, Edward M., Caddick, S.J., Churchland, P., Feng, G., Homanics, Gregg E., Lee, K.F., Leopold, David A., Miller, Cory T., Mitchell, Jude F., Mitalipov, S., Moutri, Alysson R., Movshon, J., Okano, H., Reynolds, John H., Ringach, D.L., Sejnowski, Terrence J., Silva, Afonso C., Strick, Peter L., Wu, J., Zhang, F.. Brains, Genes, and Primates. Neuron, vol.86, no.3, 617-631.

상세보기
Hua, Serenus, Jeong, Ha Neul, Dimapasoc, Lauren M., Kang, Inae, Han, Chanyoung, Choi, Jong-Soon, Lebrilla, Carlito B., An, Hyun Joo. Isomer-Specific LC/MS and LC/MS/MS Profiling of the Mouse Serum N-Glycome Revealing a Number of Novel Sialylated N-Glycans. Analytical chemistry, vol.85, no.9, 4636-4643.

상세보기
Davies, Leela R. L., Varki, Ajit. Why Is N-Glycolylneuraminic Acid Rare in the Vertebrate Brain?. Topics in current chemistry, vol.366, 31-54.

상세보기
Albach, Claus, Klein, Roger A., Schmitz, Brigitte. Do Rodent and Human Brains Have Different N-Glycosylation Patterns?. Biological chemistry, vol.382, no.2,

상세보기
Ishii, Akihiro, Ikeda, Takeshi, Hitoshi, Seiji, Fujimoto, Ichiro, Torii, Tomohiro, Sakuma, Keiichiro, Nakakita, Shin-ichi, Hase, Sumihiro, Ikenaka, Kazuhiro. Developmental changes in the expression of glycogenes and the content of N-glycans in the mouse cerebral cortex. Glycobiology, vol.17, no.3, 261-276.

상세보기
Nakakita, S.-i., Natsuka, S., Ikenaka, K., Hase, S.. Development-Dependent Expression of Complex-Type Sugar Chains Specific to Mouse Brain. The Journal of biochemistry, vol.123, no.6, 1164-1168.

상세보기
Kronewitter, Scott R., An, Hyun Joo, de Leoz, Maria Lorna, Lebrilla, Carlito B., Miyamoto, Suzanne, Leiserowitz, Gary S.. The development of retrosynthetic glycan libraries to profile and classify the human serum N-linked glycome. Proteomics, vol.9, no.11, 2986-2994.

상세보기
Chui, Daniel, Oh-Eda, Masayoshi, Liao, Yung-Feng, Panneerselvam, Krishnasamy, Lal, Anita, Marek, Kurt W, Freeze, Hudson H, Moremen, Kelley W, Fukuda, Michiko N, Marth, Jamey D. Alpha-Mannosidase-II Deficiency Results in Dyserythropoiesis and Unveils an Alternate Pathway in Oligosaccharide Biosynthesis. Cell, vol.90, no.1, 157-167.

상세보기
Lee, Jua, Hua, Serenus, Lee, Sung Hyeon, Oh, Myung Jin, Yun, Jaekyung, Kim, Jin Young, Kim, Jae-Han, Kim, Jung Hoe, An, Hyun Joo. Designation of fingerprint glycopeptides for targeted glycoproteomic analysis of serum haptoglobin: insights into gastric cancer biomarker discovery. Analytical and bioanalytical chemistry, vol.410, no.6, 1617-1629.

상세보기
Lo, N W, Shaper, J H, Pevsner, J, Shaper, N L. The expanding beta 4-galactosyltransferase gene family: messages from the databanks.. Glycobiology, vol.8, no.5, 517-526.

상세보기
Okamoto, Y., Omichi, K., Yamanaka, S., Ikenaka, K., Hase, S.. Conversion of Brain-Specific Complex Type Sugar Chains by N-Acetyl- -D-Hexosaminidase B. The Journal of biochemistry, vol.125, no.3, 537-540.

상세보기
Naito-Matsui, Yuko, Davies, Leela R. L., Takematsu, Hiromu, Chou, Hsun-Hua, Tangvoranuntakul, Pam, Carlin, Aaron F., Verhagen, Andrea, Heyser, Charles J., Yoo, Seung-Wan, Choudhury, Biswa, Paton, James C., Paton, Adrienne W., Varki, Nissi M., Schnaar, Ronald L., Varki, Ajit. Physiological Exploration of the Long Term Evolutionary Selection against Expression of N-Glycolylneuraminic Acid in the Brain. The Journal of biological chemistry, vol.292, no.7, 2557-2570.

상세보기
Bauer, Deborah, Haroutunian, Vahram, Meador-Woodruff, James H., McCullumsmith, Robert E.. Abnormal glycosylation of EAAT1 and EAAT2 in prefrontal cortex of elderly patients with schizophrenia. Schizophrenia research, vol.117, no.1, 92-98.

상세보기
Tucholski, J., Simmons, M.S., Pinner, A.L., Haroutunian, V., McCullumsmith, R.E., Meador-Woodruff, J.H.. Abnormal N-linked glycosylation of cortical AMPA receptor subunits in schizophrenia. Schizophrenia research, vol.146, no.1, 177-183.

상세보기
Mueller, Toni Marie, Haroutunian, Vahram, Meador-Woodruff, James H. N-Glycosylation of GABA_A Receptor Subunits is Altered in Schizophrenia. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, vol.39, no.3, 528-537.

상세보기
Bartel, Jörg, Krumsiek, Jan, Theis, Fabian J.. Statistical methods for the analysis of high-throughput metabolomics data. Computational and structural biotechnology journal, vol.4, no.5, e201301009-.

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