[논문]LAD1 expression is associated with the metastatic potential of colorectal cancer cells

Moon, Byul; Yang, Suk-Jin; Park, Seong Min; Lee, Sang-Hyun; Song, Kyu Sang; Jeong, Eun-Jeong; Park, Mijin; Kim, Jang-Seong; Yeom, Young Il; Kim, Jung-Ae

doi:10.1186/s12885-020-07660-0

[해외논문] LAD1 expression is associated with the metastatic potential of colorectal cancer cells 원문보기

BMC cancer, v.20 no.1, 2020년, pp.1180 -

Moon, Byul (Personalized Genomic Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141 South Korea) , Yang, Suk-Jin (Personalized Genomic Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141 South Korea) , Park, Seong Min (Personalized Genomic Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141 South Korea) , Lee, Sang-Hyun (Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141 South Korea) , Song, Kyu Sang (Department of Pathology, Chungnam National University College of Medicine, Daejeon, 34134 South Korea) , Jeong, Eun-Jeong (Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141 South Korea) , Park, Mijin (Personalized Genomic Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141 South Korea) , Kim, Jang-Seong (Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Bi) , Yeom, Young Il , Kim, Jung-Ae

Abstract ▼ AI-Helper

BackgroundAnchoring filament protein ladinin-1 (LAD1) was related to the aggressive progression of breast, lung, laryngeal and thyroid cancers. However, the association of LAD1 with colorectal cancer remained unknown. Here, to determine the relationship of LAD1 with colorectal cancer progression, we explored the effect of LAD1 loss on the malignant features of colorectal cancer cells.MethodsWe constructed LAD1-depleted cell lines and examined the effect of LAD1 deficiency on the phenotypic and molecular features of colorectal cancer cells in vitro. The function of LAD1 in metastasis in vivo was examined by establishing a spleen-to-liver metastasis mouse model. LAD1 protein expression in colorectal cancer patient specimens was assessed by immunohistochemistry of tumor microarrays.ResultsWe found that LAD1 was abundant in most colorectal cancer cells. In addition, high expression of LAD1 significantly correlated with poor patient outcome. LAD1 depletion inhibited the migration and invasion of two different colorectal cancer cell lines, SW620 and Caco-2, without affecting their proliferation. In addition, LAD1 loss led to defects in liver metastasis of SW620 cells in the mouse model. Immunohistochemistry of colorectal cancer tissues revealed LAD1 enrichment in metastatic tissues compared to that in primary tumor and normal tissues.ConclusionThese results suggest that LAD1 expression is associated with the metastatic progression of colorectal cancer by promoting the migration and invasion of cancer cells.Supplementary InformationThe online version contains supplementary material available at 10.1186/s12885-020-07660-0.

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