AbstractThe effects of cell cycle on recombinant protein production and infection yield in the baculovirus-insect cell expression system (BES) were investigated. When, at any cell cycle phase, the host cell was infected by baculovirus, the cell cycle was finally arrested at the S or G2/M phase with ...
AbstractThe effects of cell cycle on recombinant protein production and infection yield in the baculovirus-insect cell expression system (BES) were investigated. When, at any cell cycle phase, the host cell was infected by baculovirus, the cell cycle was finally arrested at the S or G2/M phase with 4n DNA. In the case of G1 or S phase-infection, cell cycle of virus-infected cells began to be arrested at S phase from 8 h post-infection or at G2/M phase from 4 h post-infection, respectively; while, in the case of M phase-infection, cell cycle was arrested at S phase after 12 h post-infection. When the host cell was infected at the G1 phase, average intracellular GFPuv fluorescence intensity was 1.3-fold higher than that at G2/M phase at 24 h post-infection. The GFPuv expression corresponded to the profile of the G1 cell cycle in the BES. Infection yield was measured by detection of intracellular DNA binding protein using immunohistochemical method within 7 h post-infection. The infection yield at G1 or S phase-infection was 1.5–1.8-fold higher than that at G2/M phase-infection.
AbstractThe effects of cell cycle on recombinant protein production and infection yield in the baculovirus-insect cell expression system (BES) were investigated. When, at any cell cycle phase, the host cell was infected by baculovirus, the cell cycle was finally arrested at the S or G2/M phase with 4n DNA. In the case of G1 or S phase-infection, cell cycle of virus-infected cells began to be arrested at S phase from 8 h post-infection or at G2/M phase from 4 h post-infection, respectively; while, in the case of M phase-infection, cell cycle was arrested at S phase after 12 h post-infection. When the host cell was infected at the G1 phase, average intracellular GFPuv fluorescence intensity was 1.3-fold higher than that at G2/M phase at 24 h post-infection. The GFPuv expression corresponded to the profile of the G1 cell cycle in the BES. Infection yield was measured by detection of intracellular DNA binding protein using immunohistochemical method within 7 h post-infection. The infection yield at G1 or S phase-infection was 1.5–1.8-fold higher than that at G2/M phase-infection.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.