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Cloning, expression, purification, and biochemical characterization of CpxR protein from pectobacterium carotovorum

Biotechnology and applied biochemistry, v.69 no.3, 2022년, pp.898 - 905  

He, Xiaoliang (School of Biological Science and Engineering Hebei University of Science and Technology Shijiazhang Hebei China) ,  Zhang, Jing (School of Biological Science and Engineering Hebei University of Science and Technology Shijiazhang Hebei China) ,  Wang, Shuai (School of Biological Science and Engineering Hebei University of Science and Technology Shijiazhang Hebei China) ,  Yang, Zi (School of Biological Science and Engineering Hebei University of Science and Technology Shijiazhang Hebei China) ,  Zhang, Huan (School of Biological Science and Engineering Hebei University of Science and Technology Shijiazhang Hebei China) ,  Zhou, Xiaohui (School of Biological Science and Engineering Hebei University of Science and Technology Shijiazhang Hebei China)

Abstract AI-Helper 아이콘AI-Helper

AbstractThe cpxR gene, encoding a new cytoplasmic response regulator, which effects virulence, biofilm formation, chemotaxis, resistance to antimicrobials, and controls soft rot, was amplified by the polymerase chain reaction, cloned into the prokaryotic expression vector pET‐15b, and express...

참고문헌 (24)

  1. Nevesinjac, Anna Z., Raivio, Tracy L.. The Cpx Envelope Stress Response Affects Expression of the Type IV Bundle-Forming Pili of EnteropathogenicEscherichiacoli. Journal of bacteriology, vol.187, no.2, 672-686.

  2. Front Microbiol Miguel AD 807 6 2015 The two‐component system CpxRA represses the expression of Salmonella virulence genes by affecting the stability of the transcriptional regulator HilD 

  3. Wolfe, Alan J., Parikh, Niyati, Lima, Bruno P., Zemaitaitis, Bozena. Signal Integration by the Two-Component Signal Transduction Response Regulator CpxR. Journal of bacteriology, vol.190, no.7, 2314-2322.

  4. Gal-Mor, Ohad, Segal, Gil. Identification of CpxR as a Positive Regulator of icm and dot Virulence Genes of Legionella pneumophila. Journal of bacteriology, vol.185, no.16, 4908-4919.

  5. Raivio, T.L.. Everything old is new again: An update on current research on the Cpx envelope stress response. Biochimica et biophysica acta, Molecular cell research, vol.1843, no.8, 1529-1541.

  6. Altman, Efrat, Segal, Gil. The Response Regulator CpxR Directly Regulates Expression of SeveralLegionella pneumophila icm/dotComponents as Well as New Translocated Substrates. Journal of bacteriology, vol.190, no.6, 1985-1996.

  7. De Wulf, Peter, McGuire, Abigail M., Liu, Xueqiao, Lin, Edmund C.C.. Genome-wide Profiling of Promoter Recognition by the Two-component Response Regulator CpxR-P in Escherichia coli. The Journal of biological chemistry, vol.277, no.29, 26652-26661.

  8. Gangaiah, Dharanesh, Zhang, Xinjun, Fortney, Kate R., Baker, Beth, Liu, Yunlong, Munson Jr, Robert S., Spinola, Stanley M.. Activation of CpxRA in Haemophilus ducreyi Primarily Inhibits the Expression of Its Targets, Including Major Virulence Determinants. Journal of bacteriology, vol.195, no.15, 3486-3502.

  9. Weatherspoon-Griffin, Natasha, Yang, Dezhi, Kong, Wei, Hua, Zichun, Shi, Yixin. The CpxR/CpxA Two-component Regulatory System Up-regulates the Multidrug Resistance Cascade to Facilitate Escherichia coli Resistance to a Model Antimicrobial Peptide. The Journal of biological chemistry, vol.289, no.47, 32571-32582.

  10. Zhou, Xiaohui, Keller, Rebecca, Volkmer, Rudolf, Krauss, Norbert, Scheerer, Patrick, Hunke, Sabine. Structural Basis for Two-component System Inhibition and Pilus Sensing by the Auxiliary CpxP Protein. The Journal of biological chemistry, vol.286, no.11, 9805-9814.

  11. J Chem Pharma Res Zhou X 396 6 6 2014 Cloning and expression of cpxA gene from Pectobacterium carotovorum subsp. Carotovorum 

  12. Hunke, Sabine, Keller, Rebecca, Müller, Volker S.. Signal integration by the Cpx‐envelope stress system. FEMS microbiology letters, vol.326, no.1, 12-22.

  13. He, X., Zhou, X., Yang, Z., Xu, L., Yu, Y., Jia, L., Li, G.. Cloning, expression and purification of d-tagatose 3-epimerase gene from Escherichia coli JM109. Protein expression and purification, vol.114, 77-81.

  14. International Conference on Biomedical Engineering and Biotechnology Yang Z 119 2012 Construction and expression of CpxP protein from plant pathogenic bacteria 

  15. He, Xiaoliang, Ren, Yuwen, Meng, Wanli, Yu, Xinran, Zhou, Xiaohui. Knocking out Analysis of the CpxP gene using Crispr/Cas9 in Escherichia coli MG1655. AMB Express, vol.10, no.1, 172-.

  16. MANSFIELD, JOHN, GENIN, STEPHANE, MAGORI, SHIMPEI, CITOVSKY, VITALY, SRIARIYANUM, MALINEE, RONALD, PAMELA, DOW, MAX, VERDIER, VALÉRIE, BEER, STEVEN V., MACHADO, MARCOS A., TOTH, IAN, SALMOND, GEORGE, FOSTER, GARY D.. Top 10 plant pathogenic bacteria in molecular plant pathology. Molecular plant pathology, vol.13, no.6, 614-629.

  17. Lee, Dong Hwan, Lim, Jeong-A, Lee, Juneok, Roh, Eunjung, Jung, Kyusuk, Choi, Minseon, Oh, Changsik, Ryu, Sangryeol, Yun, Jongchul, Heu, Sunggi. Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage. Microbiology, vol.159, no.7, 1487-1496.

  18. Raivio, Tracy L.. Envelope stress responses and Gram-negative bacterial pathogenesis. Molecular microbiology, vol.56, no.5, 1119-1128.

  19. Ma, Qun, Wood, Thomas K.. OmpA influences Escherichia coli biofilm formation by repressing cellulose production through the CpxRA two-component system. Environmental microbiology, vol.11, no.10, 2735-2746.

  20. De Wulf, Peter, Kwon, Ohsuk, Lin, E. C. C.. The CpxRA Signal Transduction System of Escherichia coli : Growth-Related Autoactivation and Control of Unanticipated Target Operons. Journal of bacteriology, vol.181, no.21, 6772-6778.

  21. Weatherspoon-Griffin, Natasha, Zhao, Guang, Kong, Wei, Kong, Ying, Morigen,, Andrews-Polymenis, Helene, McClelland, Michael, Shi, Yixin. The CpxR/CpxA Two-component System Up-regulates Two Tat-dependent Peptidoglycan Amidases to Confer Bacterial Resistance to Antimicrobial Peptide. The Journal of biological chemistry, vol.286, no.7, 5529-5539.

  22. Hirakawa, Hidetada, Nishino, Kunihiko, Hirata, Takahiro, Yamaguchi, Akihito. Comprehensive Studies of Drug Resistance Mediated by Overexpression of Response Regulators of Two-Component Signal Transduction Systems in Escherichia coli. Journal of bacteriology, vol.185, no.6, 1851-1856.

  23. Nishino, Kunihiko, Yamasaki, Seiji, Hayashi-Nishino, Mitsuko, Yamaguchi, Akihito. Effect of NlpE Overproduction on Multidrug Resistance in Escherichia coli. Antimicrobial agents and chemotherapy, vol.54, no.5, 2239-2243.

  24. Mahoney, Tara F., Silhavy, Thomas J.. The Cpx Stress Response Confers Resistance to Some, but Not All, Bactericidal Antibiotics. Journal of bacteriology, vol.195, no.9, 1869-1874.

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