[논문]Effects of Natural Rheum tanguticum on the Cell Wall Integrity of Resistant Phytopathogenic Pectobacterium carotovorum subsp. Carotovorum

Qi, Yanjiao; Wang, Mingyang; Zhang, Bo; Liu, Yue; Fan, Jiaqin; Wang, Zifan; Song, Li; Mohamed Abdul, Peer; Zhang, Hong

doi:10.3390/molecules27165291

Effects of Natural Rheum tanguticum on the Cell Wall Integrity of Resistant Phytopathogenic Pectobacterium carotovorum subsp. Carotovorum 원문보기

Molecules : a journal of synthetic chemistry and natural product chemistry, v.27 no.16, 2022년, pp.5291 -

Qi, Yanjiao (Key Laboratory for Utility of Environment-Friendly Composite Materials and Biomass, Universities of Gansu Province, Lanzhou 730000, China) , Wang, Mingyang (Key Laboratory for Utility of Environment-Friendly Composite Materials and Biomass, Universities of Gansu Province, Lanzhou 730000, China) , Zhang, Bo (Gansu Provincial Biomass Function Composites Engineering Research Center, Lanzhou 730000, China) , Liu, Yue (Gansu Provincial Biomass Function Composites Engineering Research Center, Lanzhou 730000, China) , Fan, Jiaqin (College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China) , Wang, Zifan (China-Malaysia National Joint Laboratory, Biomedical Research Center, Northwest MinZu University, Lanzhou 730000, China) , Song, Li (Gansu Hualing Dairy Co., Ltd., Ganna 747000, China) , Mohamed Abdul, Peer (Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia) , Zhang, Hong (Key Laboratory for Utility of Environment-Friendly Composite Materials and Biomass, Universities of Gan)

Abstract ▼ AI-Helper

The abuse of agricultural antibiotics has led to the emergence of drug-resistant phytopathogens. Rifampicin and streptomycin and streptomycin resistance Pectobacterium carotovorum subsp. carotovorum (PccS1) was obtained from pathological plants in a previous experiment. Rheum tanguticum, derived from the Chinese plateau area, exhibits excellent antibacterial activity against PccS1, yet the action mode has not been fully understood. In present text, the cell wall integrity of the PccS1 was tested by the variation of the cellular proteins, SDS polyacrylamide gel electrophoresis (SDS-PAGE), scanning electron microscopy (SEM) and Fourier transform infrared spectrophotometer (FTIR) characteristics. Label-free quantitative proteomics was further used to identify the DEPs in the pathogen response to treatment with Rheum tanguticum Maxim. ex Balf. extract (abbreviated as RTMBE). Based on the bioinformatics analysis of these different expressed proteins (DEPs), RTMBE mainly inhibited some key protein expressions of beta-Lactam resistance, a two-component system and phosphotransferase system. Most of these membrane proteins were extraordinarily suppressed, which was also consistent with the morphological tests. In addition, from the downregulated flagellar motility related proteins, it was also speculated that RTMBE played an essential antibacterial role by affecting the swimming motility of the cells. The results indicated that Rheum tanguticum can be used to attenuate the virulence of the drug-resistant phytopathogenic bacteria.

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Effects of Natural Rheum tanguticum on the Cell Wall Integrity of Resistant Phytopathogenic Pectobacterium carotovorum subsp. Carotovorum 원문보기

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