[논문]Method for determining microbial activity (hydrolysis of fluorescein diacetate (FDA), 490 nm)

Frlolov, O A; Terekhin, A V; Yakushev, A V; Milanovskiy, E Yu

doi:10.1088/1755-1315/1093/1/012016

Method for determining microbial activity (hydrolysis of fluorescein diacetate (FDA), 490 nm) 원문보기

IOP conference series : Earth and environmental science, v.1093 no.1, 2022년, pp.012016 -

Frlolov, O A , Terekhin, A V , Yakushev, A V , Milanovskiy, E Yu

Abstract ▼ AI-Helper

AbstractFluorescein diacetate (FDA) is used to determine microbial activity. The determination of this activity can be carried out within the frame of various study objects using soil, litter, peat, etc. This method is based on a hydrolysis reaction of converting fluorescein diacetate (FDA) to fluorescein. The concentration is usually determined using a spectrophotometer in the UV range. This article discusses the possible concentration of fluorescence in the visible spectrum (490 nm). The activity of microorganisms was determined in the Chernozem typical arable horizon. The work used two methods for determining the concentration of fluorescence by optical density. The first option is to use a calibration concentration (the dependence of optical density on the concentration of fluorescein). In this case two options were used: with heating (60 °C) and without it (25 °C). The second option is to use the Bouguer-Lambert-Beer equation and the extinction coefficient. The results were used for calculating the Michaelis-Menten constants (Km) and the maximum reaction rate (Vm). While determining the microbial activity with FDA, the concentration of fluorescein can be determined in the visible range. The more preferable way of determining the concentration of fluorescein is by using the extinction coefficient of fluorescein, rather than by plotting a calibration graph based on matrix solutions.

참고문헌 (16)

Analytical Chemistry Kramer 35 588 1963 10.1021/ac60197a027 A Substrate for the Fluorometric Determination of Lipase Activity

상세보기
Microbial ecology Swisher 6 217 1980 10.1007/BF02010387 Fluorescein diacetate hydrolysis as an estimator of microbial biomass on coniferous needle surfaces

상세보기
Applied and environmental microbiology Schnürer 43 1256 1982 10.1128/aem.43.6.1256-1261.1982 Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter

상세보기
Mar. Ecol. Prog. Ser Meyer-Reil 31 143 1986 10.3354/meps031143 Measurement of hydrolytic activity and incorporation of dissolved organic substrates by microorganisms in marine sediments

상세보기
Bulletin of environmental contamination and toxicology Reed 43 929 1989 10.1007/BF01702067 Fluorescein diacetate hydrolysis for determination of accelerated degradation of thiocarbamate herbicides

상세보기
Eurasian Soil Science Yakushev 52 1081 2019 10.1134/S1064229319090114 Microbiological Characteristics of Bare Peat Circles on Flat-Topped Peat Mounds in the North of Western Siberia

상세보기
Soil Biology and Biochemistry Adam 33 943 2001 10.1016/S0038-0717(00)00244-3 Development of a sensitive and rapid method for the measurement of total microbial activity using fluorescein diacetate (FDA) in a range of soils

상세보기
Hunter 2000
Environmental pollution Carrasco-Letelier 127 49 2004 10.1016/S0269-7491(03)00258-6 Preliminary study of prairies forested with Eucalyptus sp at the northwestern Uruguayan soils

상세보기
water research Akram 70 404 2015 10.1016/j.watres.2014.12.009 Development of an automated ballast water treatment verification system utilizing fluorescein diacetate hydrolysis as a measure of treatment efficacy

상세보기
Soil Biology and Biochemistry Rutigliano 36 1719 2004 10.1016/j.soilbio.2004.04.029 Soil microbial metabolism and nutrient status in a Mediterranean area as affected by plant cover

상세보기
Canadian journal of microbiology El-Tarabily 48 176 2002 10.1139/w01-140 Total microbial activity and microbial composition of a mangrove sediment are reduced by oil pollution at a site in the Arabian Gulf

상세보기
Enock 2002
Biochemistry Voss Jr 10 208 1971 10.1021/bi00778a003 Fluorescein Hapten and antibody active-site probe

상세보기
Moscow University Bulletin Yakushev 17 41 2009 Hydrolase activity as an indicator of the state of the microbial community of vermicompost
Soil Biology and Biochemistry Green 38 693 2006 10.1016/j.soilbio.2005.06.020 Assay for fluorescein diacetate hydrolytic activity: optimization for soil samples

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Method for determining microbial activity (hydrolysis of fluorescein diacetate (FDA), 490 nm) 원문보기

Abstract ▼ AI-Helper

참고문헌 (16)

이 논문을 인용한 문헌

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트