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NTIS 바로가기Journal of chemical engineering of Japan, v.19 no.3, 1986년, pp.227 - 231
YAMAMOTO, SHUICHI (Department of Chemical Engineering, Yamaguchi University) , NOMURA, MASAKI (Department of Chemical Engineering, Yamaguchi University) , SANO, YUJI (Department of Chemical Engineering, Yamaguchi University)
Since gel filtration chromatography with particles having a relatively small diameter and high mechanical stability (medium-performance gel filtration chromatography, MPGFC) is useful not only in scaling up the separation of proteins but also in reducing the separation time, HETP of proteins and pressure drop were measured for MPGFC (particle diameter, 44 mu m) columns of various column dimensions. The smallest column was 1. 0 cm in diameter and 30 cm in length (24 cm**3), while the largest was 9 multiplied by 90 cm (5726 cm**3). The dependences of HETP and of pressure drop per unit length on column dimensions were found to be negligible for these columns. The sample volume dependence of HETP was predicted well on the basis of the HETP at small sample volumes, when the protein concentration is low. However, when the protein concentration was increased to above 3%, HETP increased sharply.(Edited author abstract)
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