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Ultra-sensitive detection of two garlic potyviruses using a real-time fluorescent (Taqman®) RT-PCR assay

Journal of virological methods, v.118 no.1, 2004년, pp.15 - 21  

Lunello, Pablo (INIA, Department of Biotechnology, Autopista A-6, km 7, 28040 Madrid, Spain) ,  Mansilla, Carmen (INIA, Department of Biotechnology, Autopista A-6, km 7, 28040 Madrid, Spain) ,  Conci, Vilma (Instituto de Fitopatologı) ,  Ponz, Fernando (́)

Abstract AI-Helper 아이콘AI-Helper

AbstractA method for the detection of Onion yellow dwarf virus (OYDV) and Leek yellow stripe virus (LYSV), the two most prevalent garlic potyviruses, has been developed that combines IC-RT-PCR/RT-PCR with the use of TaqMan® probes. Comparisons with ELISA results obtained with identical OYDV a...

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참고문헌 (35)

  1. Arch. Virol. BlancoUrgoiti 141 12 2425 1996 10.1007/BF01718641 A strain-type clustering of Potato virus Y based on the genetic distance between isolates calculated by RFLP analysis of the amplified coat protein gene 

  2. J. Virol. Methods Boonham 101 1-2 37 2002 10.1016/S0166-0934(01)00418-9 The detection of Tomato spotted wilt virus (TSWV) in individual thrips using real-time fluorescent RT-PCR (TaqMan®) 

  3. Bos, L., 1976. Onion yellow dwarf virus. CMI/AAB Descriptions of Plant Viruses, p. 158. 

  4. Bos, L., 1981. Leek yellow stripe virus. CMI/AAB Descriptions of Plant Viruses, p. 240. 

  5. Cambra, M., Garris, M.T., Terrada, M.E., 1996. Characterizacion, diagnostico y deteccion serologica de virus. In: G. Llacer, M.M. Lopez, A. Trapero, A. Bello (Eds.), Patologı?a Vegetal, vol. 1, pp. 207-254. 

  6. Fitopatologı?a Brasilera Canavelli 23 3 354 1998 Incidencia de las virosis en cultivos de ajo Rosado Paraguayo 

  7. J. Gen. Virol. Clark 34 475 1977 10.1099/0022-1317-34-3-475 Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses 

  8. Conci, V.C., 1997. Virus y fitoplasmas de ajo. In: J.L. Burba (Ed.), 50 temas sobre produccion de ajo, vol. 3. EEA-INTA, Mendoza, Argentina, pp. 267-291. 

  9. Fitopatologı?a Brasilera Conci 24 73 1999 Serological and biological comparison of Onion yellow dwarf virus from onion and garlic in Argentina 

  10. J. Phytopathol. Conci 132 186 1991 10.1111/j.1439-0434.1991.tb00111.x Virus-free garlic (Allium sativum L.) plants obtained by thermotherapy and meristem tip culture 

  11. J. Phytopathol. Dovas 149 11-12 731 2001 10.1046/j.1439-0434.2001.00705.x Comparison of methods for virus detection in Allium spp 

  12. Bull. Eur. Assay Fish Pathol. Estepa 15 2 49 1995 Detection of trout hemorrhagic septicaemia rhabdovirus by capture with monoclonal antibodies and amplification with PCR 

  13. J. Phytopathol. Helguera 150 2 94 2002 10.1046/j.1439-0434.2002.00696.x Enhanced detection of Prune dwarf virus in peach leaves by immunocapture-reverse transcription-polymerase chain reaction with nested polymerase chain reaction (IC-RT-PCR nested PCR) 

  14. J. Virol. Methods Helguera 95 1-2 93 2001 10.1016/S0166-0934(01)00299-3 Immunocapture reverse transcription-polymerase chain reaction combined with nested PCR greatly increases the detection of Prunus necrotic ring spot virus in the peach 

  15. J. Virol. Methods Korimbocus 103 2 109 2002 10.1016/S0166-0934(01)00406-2 Improved detection of Sugarcane yellow leaf virus using a real-time fluorescent (TaqMan®) RT-PCR assay 

  16. Plant Dis. Lot 82 12 1381 1998 10.1094/PDIS.1998.82.12.1381 Effects of Onion yellow dwarf and Leek yellow stripe viruses on symptomatology and yield loss of three French garlic cultivars 

  17. J. Plant Pathol. Lunello 84 11 2002 An Argentinean isolate of Leek yellow stripe virus from leek can be transmitted to garlic 

  18. Eur. J. Plant Pathol. Mansilla 109 139 2003 10.1023/A:1022550502049 The diagnosis of the tomato variant of pepino mosaic virus: an IC-RT-PCR approach 

  19. Phytopathology Melhus 19 73 1929 A new virus disease epidemic on onions 

  20. Phytopathology Moury 90 5 522 2000 10.1094/PHYTO.2000.90.5.522 Enzyme-linked immunosorbent assay testing of shoots grown in vitro and the use of immunocapture-reverse transcription-polymerase chain reaction improve the detection of Prunus necrotic ringspot virus in rose 

  21. Phytopathology Mumford 90 5 448 2000 10.1094/PHYTO.2000.90.5.448 Detection of Potato mop top virus and Tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay 

  22. J. Virol. Methods Nolasco 45 2 201 1993 10.1016/0166-0934(93)90104-Y A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens 

  23. J. Virol. Methods Rodrı?guez 47 345 1994 10.1016/0166-0934(94)90030-2 Direct PCR detection of Foot-and-mouth disease virus 

  24. Ann. Appl. Biol. Shiboleth 138 2 187 2001 10.1111/j.1744-7348.2001.tb00101.x Molecular characterization of Onion yellow dwarf virus (OYDV) infecting garlic (Allium sativum L.) in Israel: thermotherapy inhibits virus elimination by meristem tip culture 

  25. J. Virol. Methods Stevens 68 9 1997 10.1016/S0166-0934(97)00103-1 Comparison of ELISA and RT-PCR for the detection of Beet yellows closterovirus in plants and aphids 

  26. Plant Dis. Takaichi 82 6 694 1998 10.1094/PDIS.1998.82.6.694 Four garlic viruses identified by reverse transcription-polymerase chain reaction and their regional distribution in northern Japan 

  27. Arch. Virol. Tsuneyoshi 143 6 1093 1998 10.1007/s007050050358 Differentiation of Allium carlaviruses isolated from different parts of the world based on the viral coat protein sequence 

  28. Arch. Virol. Tsuneyoshi 143 1 97 1998 10.1007/s007050050271 Nucleotide sequence analysis of virus isolates indicates the presence of three potyvirus species in Allium plants 

  29. Phytopathology Tsuneyoshi 86 3 253 1996 10.1094/Phyto-86-253 Differentiation among garlic viruses in mixed infections based on RT-PCR procedures and direct tissue blotting immunoassays 

  30. Neth. J. Plant Pathol. Van Dijk 99 2 1 1993 10.1007/BF02017734 Survey and characterization of potyviruses and their strains of Allium species 

  31. Phytoparasitica Varveri 28 2 141 2000 10.1007/BF02981743 Potato Y potyvirus detection by immunological and molecular techniques in plants and aphids 

  32. Eur. J. Plant Pathol. Verbeek 101 231 1995 10.1007/BF01874779 Efficiency of eradication of four viruses from garlic (Allium sativum) by meristem-tip culture 

  33. J. Hort. Sci. Walkey 62 211 1987 10.1080/14620316.1987.11515771 Production of virus-free garlic (Allium sativum L.) and shallot (A. ascalonicum l.) by meristem-tip culture 

  34. J. Hort. Sci. Walkey 64 1 53 1989 10.1080/14620316.1989.11515927 Agronomic evaluation of virus-free and virus-infected garlic (Allium sativum L.) 

  35. J. Virol. Methods Wetzel 33 355 1992 10.1016/0166-0934(91)90035-X A highly sensitive immunocapture polymerase chain reaction method for Plum pox potyvirus detection 

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