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NTIS 바로가기Biotechnology and bioengineering, v.89 no.2, 2005년, pp.157 - 163
Hunt, Lisa (Swiss Federal Institute of Technology Lausanne, Faculty of Basic Sciences, Institute of Biological and Chemical Process Sciences, CH1015 Lausanne, Switzerland) , Hacker, David L. (telephone: 041.21.693.6141) , Grosjean, Frédéric (fax: 041.21.693.6140) , De Jesus, Maria (Swiss Federal Institute of Technology Lausanne, Faculty of Basic Sciences, Institute of Biological and Chemical Process Sciences, CH1015 Lausanne, Switzerland) , Uebersax, Lorenz (telephone: 041.21.693.6141) , Jordan, Martin (fax: 041.21.693.6140) , Wurm, Florian M. (Swiss Federal Institute of Technology Lausanne, Faculty of Basic Sciences, Institute of Biological and Chemical Process Sciences, CH1015 Lausanne, Switzerland)
There are currently two methods for maintaining cultured mammalian cells, continuous passage at 37°C and freezing in small batches. We investigated a third approach, the “pausing” of cells for days or weeks at temperatures below 37°C in a variety of cultivation vessels. High cell...
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