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NTIS 바로가기Biotechnology and bioengineering, v.89 no.4, 2005년, pp.400 - 406
Muller, Natalie (Swiss Federal Institute of Technology, Institute of Biological Engineering and Biotechnology, Laboratory of Cellular Biotechnology, Lausanne, Switzerland) , Girard, Philippe (telephone: +41 21 693 6141) , Hacker, David L. (fax: +41 21 693 6140) , Jordan, Martin (Swiss Federal Institute of Technology, Institute of Biological Engineering and Biotechnology, Laboratory of Cellular Biotechnology, Lausanne, Switzerland) , Wurm, Florian M. (telephone: +41 21 693 6141)
For large-scale applications in biotechnology, cultivation of mammalian cells in suspension is an essential prerequisite. Typically, suspension cultures are grown in glass spinner flasks filled to less than 50% of the nominal volume. We propose a superior system for suspension cultures of mammalian ...
Annathur, Gopinath V., Pierce, Jennifer L., Combs, Rodney G., Rathore, Anurag S., Banerjee, Amit, Steinmeyer, David E.. Improvements in spinner‐flask designs for insect‐cell suspension culture. Biotechnology and applied biochemistry, vol.38, no.1, 15-18.
Brorson, Kurt, de Wit, Christina, Hamilton, Elizabeth, Mustafa, Mehnaz, Swann, Patrick G., Kiss, Robert, Taticek, Ron, Polastri, Gian, Stein, Kathryn E., Xu, Yuan. Impact of cell culture process changes on endogenous retrovirus expression. Biotechnology and bioengineering, vol.80, no.3, 257-267.
Büchs, Jochen. Introduction to advantages and problems of shaken cultures. Biochemical engineering journal, vol.7, no.2, 91-98.
Büchs, Jochen, Lotter, Stefan, Milbradt, Claudia. Out-of-phase operating conditions, a hitherto unknown phenomenon in shaking bioreactors. Biochemical engineering journal, vol.7, no.2, 135-141.
Duetz, Wouter A., Witholt, Bernard. Effectiveness of orbital shaking for the aeration of suspended bacterial cultures in square-deepwell microtiter plates. Biochemical engineering journal, vol.7, no.2, 113-115.
Duetz, Wouter A, Witholt, Bernard. Oxygen transfer by orbital shaking of square vessels and deepwell microtiter plates of various dimensions. Biochemical engineering journal, vol.17, no.3, 181-185.
Girard, P., Jordan, M., Tsao, M., Wurm, F.M.. Small-scale bioreactor system for process development and optimization. Biochemical engineering journal, vol.7, no.2, 117-119.
Girard, Philippe, Derouazi, Madiha, Baumgartner, Gwendoline, Bourgeois, Michaela, Jordan, Martin, Jacko, Barbara, Wurm, Florian M.. 100-liter transient transfection. Cytotechnology, vol.38, no.1, 15-21.
Gupta, Atul, Rao, Govind. A study of oxygen transfer in shake flasks using a non-invasive oxygen sensor. Biotechnology and bioengineering, vol.84, no.3, 351-358.
Hermann, Robert, Lehmann, Mathias, Büchs, Jochen. Characterization of gas–liquid mass transfer phenomena in microtiter plates. Biotechnology and bioengineering, vol.81, no.2, 178-186.
Jordan, Martin, Köhne, Christiane, Wurm, Florian M.. Calcium-phosphate mediated DNA transfer into HEK-293 cells in suspension: control of physicochemical parameters allows transfection in stirred media. Transfection and protein expression in mammalian cells. Cytotechnology, vol.26, no.1, 39-47.
Liu, Chao-Min, Hong, Li-Na. Development of a shaking bioreactor system for animal cell cultures. Biochemical engineering journal, vol.7, no.2, 121-125.
Maier, Ulrike, Büchs, Jochen. Characterisation of the gas–liquid mass transfer in shaking bioreactors. Biochemical engineering journal, vol.7, no.2, 99-106.
Appl Microbiol McDaniel LE 286 17 1969 10.1128/am.17.2.286-290.1969 Effect of shaking speed and type of closure on shake flask cultures
Meissner, Petra, Pick, Horst, Kulangara, Alexandra, Chatellard, Philippe, Friedrich, Kirstin, Wurm, Florian M.. Transient gene expression: Recombinant protein production with suspension-adapted HEK293-EBNA cells. Biotechnology and bioengineering, vol.75, no.2, 197-203.
Pick, H. M., Meissner, P., Preuss, A. K., Tromba, P., Vogel, H., Wurm, F. M.. Balancing GFP reporter plasmid quantity in large-scale transient transfections for recombinant anti-human Rhesus-D IgG1 synthesis. Biotechnology and bioengineering, vol.79, no.6, 595-601.
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