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Abstract ▼ AI-Helper

This experiment was conducted to develop a simple method for evaluating the difference in glufosinate tolerance levels of Agrobacterium mediated transgenic petunia lines, having bar gene. Wild type plants were discolored to yellow after 3 days of 0.72% glufosinate foliar spray, and killed within 7 d...

This experiment was conducted to develop a simple method for evaluating the difference in glufosinate tolerance levels of Agrobacterium mediated transgenic petunia lines, having bar gene. Wild type plants were discolored to yellow after 3 days of 0.72% glufosinate foliar spray, and killed within 7 days, while three transgenic lines still survived. On the other hand, in the response of transgenic lines to glufosinate they showed much differences each other: T₀1, transgenic line, was little damaged, however T₀30 and T₀57, other two lines, were injured more or less. But the injured transgenic lines soon grew new shoots again. The PAT activity of transgenic lines and wild type plants was analysed before glufosinate treatment, and the chlorophyll content was measured before and 3 days after glufosinate foliar spray. The PAT activity and chlorophyll content of transgenic plants were higher than that of non-transgenic wild type plants, but among transgenic lines much differences were observed also. The plant to have higher phosphinothricin acetyltransferase (PAT) activity showed more chlorophyll content with high correlation (r=0.738**). Therefore it was suggested that the measurement of chlorophyll content is more effective method than that of measuring PAT activity for evaluation of tolerance to glufosinate of the lines transformed with bar gene. This chlorophyll content measurement method was applied to evaluate glufosinate tolerance of 17 transgenic lines, and their glufosinate tolerance level could be quantified easily.

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