[논문]Alterations of proliferative and differentiation potentials of human embryonic stem cells during long-term culture

Park, Yong Bin; Kim, Yoon Young; Oh, Sun Kyung; Chung, Sun Gan; Ku, Seung-Yup; Kim, Seok Hyun; Choi, Young Min; Moon, Shin Yong

doi:10.3858/emm.2008.40.1.98

Alterations of proliferative and differentiation potentials of human embryonic stem cells during long-term culture 원문보기

Experimental & molecular medicine : EMM, v.40 no.1, 2008년, pp.98 - 108

Park, Yong Bin (Institute of Reproductive Medicine and Population Medical Research Center) , Kim, Yoon Young (Institute of Reproductive Medicine and Population Medical Research Center) , Oh, Sun Kyung (Institute of Reproductive Medicine and Population Medical Research Center) , Chung, Sun Gan (Central Research Institute Sam Jin Pharm. Co. Ltd. Hwasung 445-746, Korea) , Ku, Seung-Yup (Institute of Reproductive Medicine and Population Medical Research Center) , Kim, Seok Hyun (Institute of Reproductive Medicine and Population Medical Research Center) , Choi, Young Min (Institute of Reproductive Medicine and Population Medical Research Center) , Moon, Shin Yong (Institute of Reproductive Medicine and Population Medical Research Center)

Abstract ▼ AI-Helper

Human embryonic stem cells (hESCs) are considered to be able to stably maintain their haracteristics in vitro for prolonged periods, but we had previously encountered changes in proliferative ability and differentiation potential during extended culture of hESCs. Therefore, we investigated the proliferative ability and differentiation potential of hESCs during long-term culture. The hESCs, SNUhES3, were used to analyze population-doubling time, proliferation rate and differentiation potential. We classified hESCs into three groups according to culture period. Ten colonies of hESCs for each group were daily measured colony area and population-doubling time was assessed by the changes of colony area. Proliferation rate of hESCs was measured by 5-bromo-2'-deoxyuridine (BrdU) assay and telomerase activity. To evaluate differentiation potentials for hESCs, expression levels of undifferentiated and/or differentiated hESCs markers were examined by FACS, RT-PCR and immunostaining. Population-doubling time of early passage hESCs was longer than those of middle or late passage. Proliferative ability of hESCs was accelerated depending on culture periods. Cellular morphologies and the expression level of each three germ layer markers were obviously different from each passage of reattached embryoid bodies (EBs) after spontaneous differentiation. Differentiated cells of late passage expressed higher levels of undifferentiated markers such as Oct4 and SSEA4 than those of early and middle passage. But differentiated cells of early and middle passage expressed higher level of differentiated state marers, Nestin (ectoderm), Brachyury (mesoderm), HNF3β (endoderm). From these results, it can be inferred that hESCs show higher proliferative abilities and reduced differentiation potentials as the passage number increased. Therefore, we conclude that early passage hESCs could be more suitable than middle and late passage hESCs in differentiation studies.

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참고문헌 (35)

Nat Biotechnol Baker 25 207 2007 10.1038/nbt1285

상세보기
J Cell Physiol Becker 209 883 2006 10.1002/jcp.20776

상세보기
Trends Cell Biol Burdon 12 432 2002 10.1016/S0962-8924(02)02352-8

상세보기
Med Sci Monit Chiou 9 PI25 2003
Int J Biochem Cell Biol Conley 36 555 2004 10.1016/j.biocel.2003.07.003

상세보기
Nat Biotechnol Draper 22 53 2004 10.1038/nbt922

상세보기
Biol Neonate Dushnik-Levinson 67 77 1995 10.1159/000244147

상세보기
Nature Evans 292 154 1981 10.1038/292154a0

상세보기
Mol Ther Gropp 7 281 2003 10.1016/S1525-0016(02)00047-3

상세보기
Human Reproduction Ha 20 1779 2005 10.1093/humrep/deh854

상세보기
Mutat Res Harley 256 271 1991 10.1016/0921-8734(91)90018-7

상세보기
Stem Cells Kang 52 419 2006
Curr Opin Cell Biol Keller 7 862 1995 10.1016/0955-0674(95)80071-9

상세보기
J Neurochem Ko 103 1417 2007 10.1111/j.1471-4159.2007.04898.x

상세보기
Transgenic Res Longo 6 321 1997 10.1023/A:1018418914106

상세보기
Stem Cells Ma 21 111 2003 10.1634/stemcells.21-1-111

상세보기
Nature Genetics Maitra 37 1099 2005 10.1038/ng1631

상세보기
Oncogene Mostert 16 2617 1998 10.1038/sj.onc.1201787

상세보기
Stem Cells Oh 23 211 2005 10.1634/stemcells.2004-0122

상세보기
Stem Cells Oh 23 605 2005 10.1634/stemcells.2004-0297

상세보기
Cell Transplant Rambhatla 12 1 2003 10.3727/000000003783985179

상세보기
Nature Resnick 359 550 1992 10.1038/359550a0

상세보기
Nat Biotechnol Reubinoff 18 399 2000 10.1038/74447

상세보기
Human Reproduction Reubinoff 16 2187 2001 10.1093/humrep/16.10.2187

상세보기
Developmental Dynamics Rosler 229 259 2004 10.1002/dvdy.10430

상세보기
Nat Genet Rugg-Gunn 37 585 2005 10.1038/ng1556

상세보기
Oncogene Savatier 9 809 1994

상세보기
Stem Cells Segev 22 265 2004 10.1634/stemcells.22-3-265

상세보기
J Reproductive Medicine and Population Seol 20 86 2007
Annu Rev Cell Dev Biol Smith 17 435 2001 10.1146/annurev.cellbio.17.1.435

상세보기
Am J Physiol Heart Circ Physiol Snir 285 H2355 2003 10.1152/ajpheart.00020.2003

상세보기
Oncogene Stead 21 8320 2002 10.1038/sj.onc.1206015

상세보기
Science Thomson 282 1145 1998 10.1126/science.282.5391.1145

상세보기
Stem Cell Reviews White 1 131 2005 10.1385/SCR:1:2:131

상세보기
Dev Dyn Wianny 212 49 1998 10.1002/(SICI)1097-0177(199805)212:1<49::AID-AJA5>3.0.CO;2-2

상세보기

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Alterations of proliferative and differentiation potentials of human embryonic stem cells during long-term culture 원문보기

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Alterations of proliferative and differentiation potentials of human embryonic stem cells during long-term culture 원문보기

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