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Abstract ▼ AI-Helper

Ve-SCAR (sequence characterized amplified region) tightly linked to verticillium wilt resistance genes (Ve1 and Ve2) in tomato has potential to be used for marker-assisted selection (MAS) in breeding programs. In our MAS program using this SCAR marker, it was revealed that the cultivar ‘Mountain Cre...

Ve-SCAR (sequence characterized amplified region) tightly linked to verticillium wilt resistance genes (Ve1 and Ve2) in tomato has potential to be used for marker-assisted selection (MAS) in breeding programs. In our MAS program using this SCAR marker, it was revealed that the cultivar ‘Mountain Crest’ and several individual plants in a segregating population were susceptible to verticillium wilt, but carried marker genotypes of homozygous resistance. To develop a gene sequence-based marker that would universally work for diverse tomato breeding materials, the Ve1 and Ve2 genes were amplified from the resistance check ‘Hayslip’, susceptible check ‘Ace’, and ‘Mountain Crest’ and the PCR amplicons were tested for RFLP (restriction fragment length polymorphism) by using 14 restriction enzymes. Only the restriction enzyme Hinc II digestion revealed polymorphisms among those cultivars. In addition, sequence alignment of the Ve1 gene of those cultivars clearly showed that ‘Mountain Crest’ carried a second susceptible allele distinguished from other susceptible lines. The polymorphic Hinc II sites were confirmed by the gene sequence analysis and a CAPS (cleaved amplified polymorphic site) marker was developed. Compared to the previous SCAR marker, our new CAPS marker can be more reliable and efficient for the selection of verticillium wilt resistance in tomato.

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