[논문]Metallothioneins 2 and 3 contribute to the metal-adapted phenotype but are not directly linked to Zn accumulation in the metal hyperaccumulator, Thlaspi caerulescens

Hassinen, V. H.; Tuomainen, M.; Perä; niemi, S.; Schat, H.; Kä; renlampi, S. O.; Tervahauta, A. I.

doi:10.1093/jxb/ern287

[해외논문] Metallothioneins 2 and 3 contribute to the metal-adapted phenotype but are not directly linked to Zn accumulation in the metal hyperaccumulator, Thlaspi caerulescens 원문보기

Journal of experimental botany, v.60 no.1, 2009년, pp.187 - 196

Hassinen, V. H. (Department of Biosciences, University of Kuopio, Kuopio, Finland) , Tuomainen, M. (Department of Biosciences, University of Kuopio, Kuopio, Finland) , Peräniemi, S. (Department of Biosciences, University of Kuopio, Kuopio, Finland) , Schat, H. (Institute of Cellular and Molecular Biology, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands) , Kärenlampi, S. O. (Department of Biosciences, University of Kuopio, Kuopio, Finland) , Tervahauta, A. I. (Department of Biosciences, University of Kuopio, Kuopio, Finland)

Abstract ▼ AI-Helper

To study the role of metallothioneins (MTs) in Zn accumulation, the expression of TcMT2a, TcMT2b, and TcMT3 was analysed in three accessions and 15 F3 families of two inter-accession crosses of the Cd/Zn hyperaccumulator Thlaspi caerulescens, with different degrees of Zn accumulation. The highest expression levels were found in the shoots of a superior metal-accumulating calamine accession from St Laurent le Minier, with >10-fold TcMT3 expression compared with another calamine accession and a non-metallicolous accession. Moreover, F3 sibling lines from the inter-accession crosses that harboured the MT2a or MT3 allele from St Laurent le Minier had higher expression levels. However, there was no co-segregation of TcMT2a or TcMT3 expression and Zn accumulation. To examine the functions of TcMTs in plants, TcMT2a and TcMT3 were ectopically expressed in Arabidopsis. The transformant lines had reduced root length in control medium but not at high metal concentrations, suggesting that the ectopically expressed proteins interfered with the physiological availability of essential metals under limited supply. The Arabidopsis transformant lines did not show increased tolerance to Cd, Cu, or Zn, nor increased Cd or Zn accumulation. Immunohistochemical analysis indicated that in roots, MT2 protein is localized in the epidermis and root hairs of both T. caerulescens and Arabidopsis thaliana. The results suggest that TcMT2a, TcMT2b, and TcMT3 are not primarily involved in Zn accumulation as such. However, the elevated expression levels in the metallicolous accessions suggests that they do contribute to the metal-adapted phenotype, possibly through improving Cu homeostasis at high Zn and Cd body burdens. Alternatively, they might function as hypostatic enhancers of Zn or Cd tolerance.

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참고문헌 (30)

Abramoff MD Magelhaes PJ Ram SJ Image processing with ImageJ Biophotonics International 2004 11 36 42

상세보기
Assunção AGL Ten Bookum WM Nelissen HJM Vooijs R Schat H Ernst WHO Differential metal-specific tolerance and accumulation patterns among Thlaspi caerulescens populations originating from different soil types New Phytologist 2003a 159 411 419

상세보기
Assunção AGL Ten Bookum WM Nelissen HJM Vooijs R Schat H Ernst WHO A cosegregation analysis of zinc (Zn) accumulation and Zn tolerance in the Zn hyperaccumulator Thlaspi caerulescens New Phytologist 2003b 159 383 390

상세보기
Clough SJ Bent AF Floral dip: a simplified method for Agrobacterium -mediated transformation of Arabidopsis thaliana The Plant Journal 1998 16 735 743 10069079

상세보기
Cobbett C Goldsbrough P Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis Annual Review of Plant Biology 2002 53 159 182

상세보기
Deniau AX Pieper B Ten Bookum WM Lindhout P Aarts MG Schat H QTL analysis of cadmium and zinc accumulation in the heavy metal hyperaccumulator Thlaspi caerulescens Theoretical and Applied Genetics 2006 113 907 920 16850314
Domènech J Mir G Huguet G Capdevila M Molinas M Atrian S Plant metallothionein domains: functional insight into physiological metal binding and protein folding Biochimie 2006 88 583 593 16377055

상세보기
Domènech J Orihuela R Mir G Molinas M Atrian S Capdevila M The Cd II -binding abilities of recombinant Quercus suber metallothionein: bridging the gap between phytochelatins and metallothioneins Journal of Biological Inorganic Chemistry 2007 12 867 882 17503092

상세보기
Eide DJ Clark S Nair TM Gehl M Gribskov M Guerinot ML Harper JF Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae Genome Biology 2005 6 R77 16168084
Guo W Bundithya W Goldsbrough PB Characterization of the Arabidopsis metallothionein gene family: tissue-specific expression and induction during senescence and in response to copper New Phytologist 2003 159 369 381

상세보기
Guo W Meetam M Goldsbrough PB Examining the specific contributions of individual arabidopsis metallothioneins to copper distribution and metal tolerance Plant Physiology 2008 146 1697 1706 18287486

상세보기
Hassinen VH Tervahauta AI Halimaa P Plessl M Aarts MGM Peräniemi S Servomaa K Schat H Kärenlampi SO Isolation of Zn-responsive genes from two accessions of the hyperaccumulator plant Thlaspi caerulescens Planta 2007 225 977 989 17013613

상세보기
Jack E Hakvoort HWJ Reumer A Verkleij JAC Schat H Ernst WHO Real-time PCR analysis of metallothionein-2b expression in metallicolous and non-metallicolous populations of Silene vulgaris (Moench) Garcke Environmental and Experimental Botany 2007 59 84 91

상세보기
Lee J Shim D Song WY Hwang I Lee Y Arabidopsis metallothioneins 2a and 3 enhance resistance to cadmium when expressed in Vicia faba guard cells Plant Molecular Biology 2004 54 805 815 15604653

상세보기
Livak KJ Schmittgen TD Analysis of relative gene expression data using realtime quantitative PCR and the 2 –ΔΔ C T method Methods 2001 25 402 408 11846609
Meerts P Van Isacker N Heavy metal tolerance and accumulation in metallicolous and non-metallicolous populations of Thlaspi caerulescens from continental Europe Plant Ecology 1997 133 221 231

상세보기
Mir G Domènech J Huguet G Guo W-J Goldsbrough P Atrian S Molinas M A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress Journal of Experimental Botany 2004 55 2483 2493 15448172

상세보기
Murphy A Taiz L Comparison of metallothionein gene expression and nonprotein thiols in ten Arabidopsis ecotypes. Correlation with copper tolerance Plant Physiology 1995 109 945 954 8552721

상세보기
Murphy A Zhou J Goldsbrough PB Taiz L Purification and immunological identification of metallothioneins 1 and 2 from Arabidopsis thaliana Plant Physiology 1997 113 1293 1301 9112777

상세보기
Pagani A Villarreal L Capdevila M Atrian S The Saccharomyces cerevisiae Crs5 metallothionein metal-binding abilities and its role in the response to zinc overload Molecular Microbiology 2007 63 256 269 17163970

상세보기
Roosens NH Bernard C Leplae R Verbruggen N Evidence for copper homeostasis function of metallothionein (MT3) in the hyperaccumulator Thlaspi caerulescens FEBS Letters 2004 577 9 16 15527754

상세보기
Roosens NH Leplae R Bernard C Verbruggen N Variations in plant metallothioneins: the heavy metal hyperaccumulator Thlaspi caerulescens as a study case Planta 2005 222 716 729 16052319

상세보기
Schat H Vooijs R Kuiper E Identical major gene loci for heavy metal tolerances that have independently evolved in different local populations and subspecies of Silene vulgaris Evolution 1996 50 1888 1895

상세보기
van de Mortel JE Villanueva LA Schat H Kwekkeboom J Coughlan S Moerland PD Ver Loren van Themaat E Koornneef M Aarts MGM Large expression differences in genes for iron and zinc homeostasis, stress response, and lignin biosynthesis distinguish roots of Arabidopsis thaliana and the related metal hyperaccumulator Thlaspi caerulescens Plant Physiology 2006 142 1127 1147 16998091
van Hoof NA Hassinen VH Hakvoort HW Ballintijn KF Schat H Verkleij JA Ernst WH Kärenlampi SO Tervahauta AI Enhanced copper tolerance in Silene vulgaris (Moench) Garcke populations from copper mines is associated with increased transcript levels of a 2b-type metallothionein gene Plant Physiology 2001 126 1519 1526 11500550

상세보기
Zha HG Jiang RF Zhao FJ Vooijs R Schat H Barker JHA McGrath SP Co-segregation analysis of cadmium and zinc accumulation in Thlaspi caerulescens interecotypic crosses New Phytologist 2004 163 299 312

상세보기
Zhao FJ Hamon RE Lombi E McLaughlin MJ McGraph SP Characteristics of cadmium uptake in two contrasting ecotypes of the hyperaccumulator Thlaspi caerulescens Journal of Experimental Botany 2002 53 535 543 11847252
Zhigang A Cuijie L Yuangang Z Yejie D Wachter A Gromes R Rausch T Expression of BjMT2, a metallothionein 2 from Brassica juncea , increases copper and cadmium tolerance in Escherichia coli and Arabidopsis thaliana , but inhibits root elongation in Arabidopsis thaliana seedlings Journal of Experimental Botany 2006 57 3575 3582 16957018
Zhou J Goldsbrough PB Functional homologues of fungal metallothionein genes from Arabidopsis The Plant Cell 1994 6 875 884 8061521
Zimeri AM Dhankher OP McCaig B Meagher RB The plant MT1 metallothioneins are stabilized by binding cadmium and are required for cadmium tolerance and accumulation Plant Molecular Biology 2005 58 839 855 16240177

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[해외논문] Metallothioneins 2 and 3 contribute to the metal-adapted phenotype but are not directly linked to Zn accumulation in the metal hyperaccumulator, Thlaspi caerulescens 원문보기

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