Abstract ▼ AI-Helper

Objective: Although the chlorohexidine was known to be a good agent for the prevention and control of dental disease, it has also side effect, that is hypersensitivity and staining of mouth etc. Extracts of natural plant could be a good choice because of almost negligible side effects. In our previo...

Objective: Although the chlorohexidine was known to be a good agent for the prevention and control of dental disease, it has also side effect, that is hypersensitivity and staining of mouth etc. Extracts of natural plant could be a good choice because of almost negligible side effects. In our previous study, the crude extract from the stem of Akebia quinata showed strong antibacterial activity to oral bacteria related dental disease. This study was designed to investigate antibacterial effect in another site of Akebia quinata. The fruit of Akebia quinata was studied to investigate the effect to oral environmental system. Material and Method: The dried and finely ground Akebia quinata fruit was extracted with methanol and then the crude extract was concentrated under vacuum. The crude extract was dissolved in 70% aqueous methanol and successively separated with n-hexane. The resultant extracts were concentrated and were lyophilized. The dried extract were dissolved in dimethyl sulfoxide (DMSO) to a concentration of 100 ㎎/㎖ just prior to performance of the assays. The extracts were tested for antimicrobial activity against 18 bacterial strains. S. mutans and S. sobrinus were tested in the assays time-kill at above-MBC levels. Results: Among the extracts of Akebia quinata, the hexane fraction showed the most effective antibacterial action. The MIC of hexane fraction in S. cricetus, S. mutans, S. sobrinus defined from 0.04 to 0.08 ㎎/㎖, MBC of it defined from 0.08 to 2.5 ㎎/㎖. The hexane fraction killed about 1-2 log10 CFU/㎖ of S. mutans and S. sobrinus after 24h of exposure. The time-kill effects of hexane fraction increased in a treatment time-dependent manner. But, it was not a concentration-dependent manner. Conclusion: MIC level for Akebia quinata fruit of antimicrobial effect to S. cricetus, S. mutans, S. sobrinus was from 0.04 to 0.08 ㎎/㎖, and MBC level of it was from 0.08 to 2.5 ㎎/㎖.

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