Abstract ▼ AI-Helper

To identify pre-harvest sprouting (PHS) tolerant wheat varieties (or lines) and to understand their mechanism of resistance, three methods, i. e. , spike germination rate (SGR) , germination index (GI), and molecular marker VplB3 , were used to screen the PHS tolerance of the 33 new wheat lines in c...

To identify pre-harvest sprouting (PHS) tolerant wheat varieties (or lines) and to understand their mechanism of resistance, three methods, i. e. , spike germination rate (SGR) , germination index (GI), and molecular marker VplB3 , were used to screen the PHS tolerance of the 33 new wheat lines in comparison with two red grain tolerant cultivars Jingdong 8 and Jing 9428, and two white grain susceptible cultivars Jing 411 and Zhongyou 9507. Seven lines with lower SGR than the average value of PHS tolerant cultivars (12. 7%) were identified, among which five lines had red grain and two lines'.had white grain. Only one red grain line CA0489 exhibited lower GI (12. 8%) than the mean value of. PHS tolerant cultivars (13. 2%). Three types of DNA fragments, 845 bp, 569 bp and 652 bp, could be amplified by STS molecular marker VplB3 with frequencies of 3% , 18% and 79% in all lines, and which belonged to resistance genotype VplBb , VplBc and susceptible genotype VplBa , respectively. The red grain PHS resistance line CA0489 possessed both VplBh tolerant gene and red grain related dormancy gene; red grain PHS resistant line CA0481 had VplBc tolerant 'gene, but the mechanisms of the rest three red grain PHS resistant lines remain unclear. The two white grain PHS resistance lines CAO5O9 and CA0459 did not contain Vpl tolerant gene" their PHS tolerance might be related to the characters of spike and the stayers excreted from glumes.

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