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Abstract ▼ AI-Helper

Microorganisms often develop the way to survive against antibiotics and other antimicrobial drugs as people overuse them. Antibiotic resistant microorganisms could threaten human health with deadly disease. In nature, microorganisms need few nutrients to stay alive. In laboratory conditions, however...

Microorganisms often develop the way to survive against antibiotics and other antimicrobial drugs as people overuse them. Antibiotic resistant microorganisms could threaten human health with deadly disease. In nature, microorganisms need few nutrients to stay alive. In laboratory conditions, however, microbes from environmental samples are normally grown in nutrient-rich culture media. Because of this, the currently-used and typical cultivation methods are not suitable for microbial risk assessment of environment water samples. The purpose of this research is to develop new cultivation method to detect oligotrophic antibiotic resistant bacteria. For this, one of general high nutrient media, LB, was diluted in series, and then amended with tetracycline at 100 ㎍/mL. For quantification of antibiotic resistant bacteria, a standard spread plate method was modified using the antibiotic-amended diluted LB plates. The ability of the new method to detect oligotrophics and antibiotic resistant microorganisms was confirmed when analyzing drinking water and swine manure wastewater, respectively, followed by 16S rDNA analysis of cultivated isolates from the plates. When this method was applied in evaluating antibiotic resistant microbial risk in river water, microbial risk seemed to be low. However, in the case of Asian dust rain, the relative abundance of antibiotic resistant microorganisms in total culturable bacteria was higher than that for swine manure. This suggests that microbial risk due to antibiotic resistant microbes could be high in the Asian dust containing rain water.

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