Glucose Oxidase로 손상된 배양 C6 Glioma 세포에 대한 인동덩굴 추출물의 항산화 효과 Antioxidative Effect of Lonicera japonica L. Extract on Cultured C6 Glioma Cells Damaged by Glucose Oxidase
인동과에 속하는 인동덩굴(Lonicera japonica Thunb., LJ) 추출물이 활성산소의 일종인 glucose oxidase(GO)에 미치는 영향을 배양 C6 glioma 세포를 재료로 알아보았으며, 또한 LJ 추출물의 항산화 효과를 전자공여능(DPPH-radical scavenging activity)을 비롯하여 항산화 효소의 하나인 SOD의 활성과 비슷한 superoxide dismutase(SOD)-유사활성(SOD-like activity), 지질과산화 억제능(lipid peroxidation inhibitory activity) 및 lactate dehydrogenase(DH) 활성에 의하여 조사하였다. 본 연구에서 GO는 처리 농도 의존적으로 대조군에 비하여 배양 C6 glioma 세포의 생존율을 유의하게 감소시킴으로서 세포독성을 나타냈으며(P<0.01), 이 때 XTT₅₀값은 35 mUㆍmL⁻¹에서 나타났다. 또한, LJ 추출물은 GO에 의하여 감소된 세포생존율을 유의하게 증가시킴으로써 GO의 세포독성을 방어하였다. 한편, LJ 추출물은 전자공여능을 비롯한 SOD-유사활성, 지질과산화 억제능 및 LDH의 활성 감소를 보임으로서 항산화 효과를 나타냈다. 이상의 결과로부터 LJ 추출물은 항산화 효과에 의하여 GO의 세포독성을 유효하게 방어한 것으로 나타났다.
인동과에 속하는 인동덩굴(Lonicera japonica Thunb., LJ) 추출물이 활성산소의 일종인 glucose oxidase(GO)에 미치는 영향을 배양 C6 glioma 세포를 재료로 알아보았으며, 또한 LJ 추출물의 항산화 효과를 전자공여능(DPPH-radical scavenging activity)을 비롯하여 항산화 효소의 하나인 SOD의 활성과 비슷한 superoxide dismutase(SOD)-유사활성(SOD-like activity), 지질과산화 억제능(lipid peroxidation inhibitory activity) 및 lactate dehydrogenase(DH) 활성에 의하여 조사하였다. 본 연구에서 GO는 처리 농도 의존적으로 대조군에 비하여 배양 C6 glioma 세포의 생존율을 유의하게 감소시킴으로서 세포독성을 나타냈으며(P<0.01), 이 때 XTT₅₀값은 35 mUㆍmL⁻¹에서 나타났다. 또한, LJ 추출물은 GO에 의하여 감소된 세포생존율을 유의하게 증가시킴으로써 GO의 세포독성을 방어하였다. 한편, LJ 추출물은 전자공여능을 비롯한 SOD-유사활성, 지질과산화 억제능 및 LDH의 활성 감소를 보임으로서 항산화 효과를 나타냈다. 이상의 결과로부터 LJ 추출물은 항산화 효과에 의하여 GO의 세포독성을 유효하게 방어한 것으로 나타났다.
To evaluate the protective effect of Lonicera japonica Thunb.(LJ) extract on glucose oxidase(GO)-mediated cytotoxicity in cultured C6 glioma cells, and the antioxidative effect of LJ extract, colorimetric assays such as the cell viability, DPPH-radical scavenging activity, superoxide dismutase(SOD...
To evaluate the protective effect of Lonicera japonica Thunb.(LJ) extract on glucose oxidase(GO)-mediated cytotoxicity in cultured C6 glioma cells, and the antioxidative effect of LJ extract, colorimetric assays such as the cell viability, DPPH-radical scavenging activity, superoxide dismutase(SOD)-like activity, lipid peroxidation(LP) inhibitory activity and lactate dehydrogenase(LDH) activity were performed. For this study, C6 glioma cells were treated with the media containing 15∼35 mUㆍmL⁻¹ GO for 4 hours, respectively. In this study, GO significantly decreased cell viability in dose-dependent manner, and the XTT₅₀ value was measured at 40 mUㆍmL⁻¹ GO. In the effect of LJ extract on GO-mediated cytotoxicity, LJ extract significantly increased the cell viability which was decreased by GO. In the antioxidative effects, LJ extract showed the DPPH-radical scavenging activity, SOD-like activity, the inhibitory activity of LP and the decrease of LDH activity. From these results, it is suggested that the GO showed cytotoxicity on cultured C6 glioma cells by the decrease of cell viability, and LJ extract was effective in the protection of GO-mediated cell injury by antioxidative effect.
To evaluate the protective effect of Lonicera japonica Thunb.(LJ) extract on glucose oxidase(GO)-mediated cytotoxicity in cultured C6 glioma cells, and the antioxidative effect of LJ extract, colorimetric assays such as the cell viability, DPPH-radical scavenging activity, superoxide dismutase(SOD)-like activity, lipid peroxidation(LP) inhibitory activity and lactate dehydrogenase(LDH) activity were performed. For this study, C6 glioma cells were treated with the media containing 15∼35 mUㆍmL⁻¹ GO for 4 hours, respectively. In this study, GO significantly decreased cell viability in dose-dependent manner, and the XTT₅₀ value was measured at 40 mUㆍmL⁻¹ GO. In the effect of LJ extract on GO-mediated cytotoxicity, LJ extract significantly increased the cell viability which was decreased by GO. In the antioxidative effects, LJ extract showed the DPPH-radical scavenging activity, SOD-like activity, the inhibitory activity of LP and the decrease of LDH activity. From these results, it is suggested that the GO showed cytotoxicity on cultured C6 glioma cells by the decrease of cell viability, and LJ extract was effective in the protection of GO-mediated cell injury by antioxidative effect.
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