[논문]Investigation of cellular responses upon interaction with silver nanoparticles

Subbiah, Ramesh; Jeon, Seong Beom; Park, Kwideok; Ahn, Sang Jung; Yun, Kyusik

doi:10.2147/IJN.S88508

Investigation of cellular responses upon interaction with silver nanoparticles 원문보기

International journal of nanomedicine, v.10 specal issue, 2015년, pp.191 - 201

Subbiah, Ramesh (Center for Biomaterials, Korea Institute of Science and Technology, Seoul, Republic of Korea) , Jeon, Seong Beom (Department of Bionanotechnology, Gachon University, Gyeonggi-do, Republic of Korea) , Park, Kwideok (Center for Biomaterials, Korea Institute of Science and Technology, Seoul, Republic of Korea) , Ahn, Sang Jung (Centre for Advanced Instrumentation, Korea Research Institute of Standard and Science, Korea University of Science and Technology, Daejeon, Republic of Korea) , Yun, Kyusik (Department of Bionanotechnology, Gachon University, Gyeonggi-do, Republic of Korea)

Abstract ▼ AI-Helper

In order for nanoparticles (NPs) to be applied in the biomedical field, a thorough investigation of their interactions with biological systems is required. Although this is a growing area of research, there is a paucity of comprehensive data in cell-based studies. To address this, we analyzed the physicomechanical responses of human alveolar epithelial cells (A549), mouse fibroblasts (NIH3T3), and human bone marrow stromal cells (HS-5), following their interaction with silver nanoparticles (AgNPs). When compared with kanamycin, AgNPs exhibited moderate antibacterial activity. Cell viability ranged from ≤80% at a high AgNPs dose (40 µg/mL) to >95% at a low dose (10 µg/mL). We also used atomic force microscopy-coupled force spectroscopy to evaluate the biophysical and biomechanical properties of cells. This revealed that AgNPs treatment increased the surface roughness (P<0.001) and stiffness (P<0.001) of cells. Certain cellular changes are likely due to interaction of the AgNPs with the cell surface. The degree to which cellular morphology was altered directly proportional to the level of AgNP-induced cytotoxicity. Together, these data suggest that atomic force microscopy can be used as a potential tool to develop a biomechanics-based biomarker for the evaluation of NP-dependent cytotoxicity and cytopathology.

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Investigation of cellular responses upon interaction with silver nanoparticles 원문보기

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Investigation of cellular responses upon interaction with silver nanoparticles 원문보기

Abstract ▼ AI-Helper

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