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[해외논문] A removable virus vector suitable for plant genome editing 원문보기

The Plant journal : for cell and molecular biology, v.91 no.3, 2017년, pp.558 - 561  

Chujo, Tetsuya (Plant and Microbial Research Unit, Division of Plant and Microbial Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 2‐) ,  Yoshikawa, Manabu (1‐) ,  Ariga, Hirotaka (2 Kannondai, Tsukuba, Ibaraki, 305‐) ,  Endo, Masaki (8602, Japan) ,  Toki, Seiichi (Plant and Microbial Research Unit, Division of Plant and Microbial Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 2‐) ,  Ishibashi, Kazuhiro (1‐)

Abstract AI-Helper 아이콘AI-Helper

SummaryPlant genome editing is achieved by the expression of sequence‐specific nucleases (SSNs). RNA virus vector‐mediated expression of SSNs is a promising approach for transgene integration‐free targeted mutagenesis in plants. However, the removal of virus vectors from infected p...

Abstract

Significance StatementHere we developed a removable tomato mosaic virus vector by inserting the target sequence of an inducible host endogenous microRNA. Expression of a sequence‐specific nuclease using the virus vector in tobacco leaves followed by removal of the virus vector from regenerated genome‐edited shoots was demonstrated.

Keyword

참고문헌 (20)

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  2. Ali, Zahir, Abul-faraj, Aala, Piatek, Marek, Mahfouz, Magdy M. Activity and specificity of TRV-mediated gene editing in plants. Plant signaling & behavior, vol.10, no.10, e1044191-.

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  6. Bonnet, Eric, Wuyts, Jan, Rouzé, Pierre, Van de Peer, Yves. Detection of 91 potential conserved plant microRNAs in Arabidopsis thaliana and Oryza sativa identifies important target genes. Proceedings of the National Academy of Sciences of the United States of America, vol.101, no.31, 11511-11516.

  7. Dugas, Diana V., Bartel, Bonnie. Sucrose induction of Arabidopsis miR398 represses two Cu/Zn superoxide dismutases. Plant molecular biology, vol.67, no.4, 403-417.

  8. Feng, Xiao-Ming, Qiao, Yu, Mao, Ke, Hao, Yu-Jin. Ectopic overexpression of AtmiR398b gene in tobacco influences seed germination and seedling growth. Plant cell, tissue and organ culture, vol.102, no.1, 53-59.

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  12. KURODA, Masaharu, KIMIZU, Mayumi, MIKAMI, Chikako. A Simple Set of Plasmids for the Production of Transgenic Plants. Bioscience, biotechnology, and biochemistry, vol.74, no.11, 2348-2351.

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  14. Marton, Ira, Zuker, Amir, Shklarman, Elena, Zeevi, Vardit, Tovkach, Andrey, Roffe, Suzy, Ovadis, Marianna, Tzfira, Tzvi, Vainstein, Alexander. Nontransgenic Genome Modification in Plant Cells. Plant physiology, vol.154, no.3, 1079-1087.

  15. Miyashita, Shuhei, Ishibashi, Kazuhiro, Kishino, Hirohisa, Ishikawa, Masayuki, Sugden, Bill. Viruses Roll the Dice: The Stochastic Behavior of Viral Genome Molecules Accelerates Viral Adaptation at the Cell and Tissue Levels. PLoS biology, vol.13, no.3, e1002094-.

  16. Nakazono, Mikio, Qiu, Fang, Borsuk, Lisa A., Schnable, Patrick S.. Laser-Capture Microdissection, a Tool for the Global Analysis of Gene Expression in Specific Plant Cell Types: Identification of Genes Expressed Differentially in Epidermal Cells or Vascular Tissues of Maize[W]. The Plant cell, vol.15, no.3, 583-596.

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  19. Yoshikawa, Manabu, Iki, Taichiro, Numa, Hisataka, Miyashita, Kyoko, Meshi, Tetsuo, Ishikawa, Masayuki. A Short Open Reading Frame Encompassing the MicroRNA173 Target Site Plays a Role in trans-Acting Small Interfering RNA Biogenesis. Plant physiology, vol.171, no.1, 359-368.

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