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[해외논문] A removable virus vector suitable for plant genome editing 원문보기

The Plant journal : for cell and molecular biology, v.91 no.3, 2017년, pp.558 - 561  

Chujo, Tetsuya (Plant and Microbial Research Unit, Division of Plant and Microbial Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 2‐) ,  Yoshikawa, Manabu (1‐) ,  Ariga, Hirotaka (2 Kannondai, Tsukuba, Ibaraki, 305‐) ,  Endo, Masaki (8602, Japan) ,  Toki, Seiichi (Plant and Microbial Research Unit, Division of Plant and Microbial Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 2‐) ,  Ishibashi, Kazuhiro (1‐)

Abstract AI-Helper 아이콘AI-Helper

SummaryPlant genome editing is achieved by the expression of sequence‐specific nucleases (SSNs). RNA virus vector‐mediated expression of SSNs is a promising approach for transgene integration‐free targeted mutagenesis in plants. However, the removal of virus vectors from infected p...

Abstract

Significance StatementHere we developed a removable tomato mosaic virus vector by inserting the target sequence of an inducible host endogenous microRNA. Expression of a sequence‐specific nuclease using the virus vector in tobacco leaves followed by removal of the virus vector from regenerated genome‐edited shoots was demonstrated.

주제어

참고문헌 (20)

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  8. Feng, Xiao-Ming, Qiao, Yu, Mao, Ke, Hao, Yu-Jin. Ectopic overexpression of AtmiR398b gene in tobacco influences seed germination and seedling growth. Plant cell, tissue and organ culture, vol.102, no.1, 53-59.

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  13. Liu, Qikun, Wang, Feng, Axtell, Michael J.. Analysis of Complementarity Requirements for Plant MicroRNA Targeting Using a Nicotiana benthamiana Quantitative Transient Assay. The Plant cell, vol.26, no.2, 741-753.

  14. Marton, Ira, Zuker, Amir, Shklarman, Elena, Zeevi, Vardit, Tovkach, Andrey, Roffe, Suzy, Ovadis, Marianna, Tzfira, Tzvi, Vainstein, Alexander. Nontransgenic Genome Modification in Plant Cells. Plant physiology, vol.154, no.3, 1079-1087.

  15. Miyashita, Shuhei, Ishibashi, Kazuhiro, Kishino, Hirohisa, Ishikawa, Masayuki, Sugden, Bill. Viruses Roll the Dice: The Stochastic Behavior of Viral Genome Molecules Accelerates Viral Adaptation at the Cell and Tissue Levels. PLoS biology, vol.13, no.3, e1002094-.

  16. Nakazono, Mikio, Qiu, Fang, Borsuk, Lisa A., Schnable, Patrick S.. Laser-Capture Microdissection, a Tool for the Global Analysis of Gene Expression in Specific Plant Cell Types: Identification of Genes Expressed Differentially in Epidermal Cells or Vascular Tissues of Maize[W]. The Plant cell, vol.15, no.3, 583-596.

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  19. Yoshikawa, Manabu, Iki, Taichiro, Numa, Hisataka, Miyashita, Kyoko, Meshi, Tetsuo, Ishikawa, Masayuki. A Short Open Reading Frame Encompassing the MicroRNA173 Target Site Plays a Role in trans-Acting Small Interfering RNA Biogenesis. Plant physiology, vol.171, no.1, 359-368.

  20. Zhu, Cheng, Ding, Yanfei, Liu, Haili. MiR398 and plant stress responses. Physiologia plantarum, vol.143, no.1, 1-9.

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