[논문]Pulsed electromagnetic field induces Ca2+-dependent osteoblastogenesis in C3H10T1/2 mesenchymal cells through the Wnt-Ca2+/Wnt-β-catenin signaling pathway

Wu, Shaoyu; Yu, Qiang; Lai, Anli; Tian, Jing

doi:10.1016/j.bbrc.2018.06.066

Pulsed electromagnetic field induces Ca2+-dependent osteoblastogenesis in C3H10T1/2 mesenchymal cells through the Wnt-Ca2+/Wnt-β-catenin signaling pathway

Biochemical and biophysical research communications, v.503 no.2, 2018년, pp.715 - 721

Wu, Shaoyu (Zhujiang Hospital of Southern Medical University) , Yu, Qiang (Zhujiang Hospital of Southern Medical University) , Lai, Anli (Department of Orthopaedics, Zhujiang Hospital, Southern Medical University) , Tian, Jing (Department of Orthopaedics, Zhujiang Hospital, Southern Medical University)

Abstract ▼ AI-Helper

Abstract Pulsed electromagnetic fields (PEMFs) are effective in healing fractures and improving osteoporosis. However, their effect on mesenchymal cells remains largely unknown. In this study, the effects of PEMF on osteoblastogenesis and its underlying molecular signaling mechanisms were systematically investigated in C3H10T1/2 cells. C3H10T1/2 mesenchymal cells were exposed to 30-Hz PEMF bursts at various intensities for 3 consecutive days. The optimal PEMF exposure (30 Hz, 1 mT, 2 h/day) was applied in subsequent experiments. Our results suggest that intracellular [Ca2+]i in C3H10T1/2 cells can be upregulated upon exposure to PEMF and that PEMF-induced C3H10T1/2 cell differentiation was Ca2+-dependent. The pro-osteogenic effect of PEMF on Ca2+-dependent osteoblast differentiation was then verified by alkaline phosphatase (ALP) and von Kossa staining. Furthermore, PEMF promoted the gene expression and protein synthesis of the Wnt/β-catenin pathway. Increased [Ca2+]i in the nucleoplasm was followed by the mobilization and translocation of β-catenin into the nucleus in C3H10T1/2 cells. A model of Wnt/β-catenin signaling and the Wnt/Ca2+ signaling network is proposed. Taken together, these findings indicated for the first time that PEMF induces osteoblastogenesis through increased intracellular [Ca2+]i and the Wnt-Ca2+/Wnt-β-catenin signaling pathway in C3H10T1/2 mesenchymal cells. Highlights C3H10T1/2 cell proliferation can be greatly promoted by PEMF exposure. C3H10T1/2 cell differentiation into osteoblast can be induced upon the stimulation of PEMF. Intracellular [Ca2+]i up-regulation was observed during the process of PEMF-induced C3H10T1/2 cell differentiation. PEMF-induced C3H10T1/2 cell differentiation was Ca2+-dependent. PEMF-induced [Ca2+]i elevation suggested a combination of Wnt/Ca2+ and Wnt/β-catenin signaling pathways. Graphical abstract [DISPLAY OMISSION]

주제어

참고문헌 (25)

Semin. Cell Dev. Biol. Reid 19 473 2008 10.1016/j.semcdb.2008.08.002 Anti-resorptive therapies for osteoporosis

상세보기
J. Cell. Biochem. Brommage 116 2139 2015 10.1002/jcb.25179 Genetic approaches to identifying novel osteoporosis drug targets

상세보기
Pain Fulfaro 78 157 1998 10.1016/S0304-3959(98)00135-3 The role of bisphosphonates in the treatment of painful metastatic bone disease: a review of phase III trials

상세보기
Science Turgeon 304 1269 2004 10.1126/science.1096725 Hormone therapy: physiological complexity belies therapeutic simplicity

상세보기
Electromagn. Biol. Med. Li 31 356 2012 10.3109/15368378.2012.662194 Effects of 50 Hz pulsed electromagnetic fields on the growth and cell cycle arrest of mesenchymal stem cells: an in vitro study

상세보기
Int. Orthop. Li 35 1875 2011 10.1007/s00264-011-1245-3 Effects of pulsed electromagnetic fields on cartilage apoptosis signalling pathways in ovariectomised rats

상세보기
Int. J. Immunopathol. Pharmacol. Chalidis 24 17 2011 10.1177/03946320110241S204 Stimulation of bone formation and fracture healing with pulsed electromagnetic fields: biologic responses and clinical implications

상세보기
J. Bone Miner. Res. Adami 21 1565 2006 10.1359/jbmr.060715 Fracture incidence and characterization in patients on osteoporosis treatment: the ICARO study

상세보기
J. Cell Biol. Ooashi 170 1159 2005 10.1083/jcb.200503157 Cell adhesion molecules regulate Ca2+-mediated steering of growth cones via cyclic AMP and ryanodine receptor type 3

상세보기
Cell Calcium Grassi 35 307 2004 10.1016/j.ceca.2003.09.001 Effects of 50 Hz electromagnetic fields on voltage-gated Ca2+ channels and their role in modulation of neuroendocrine cell proliferation and death

상세보기
Bioelectromagnetics Lisi 27 641 2006 10.1002/bem.20255 Extremely low frequency electromagnetic field exposure promotes differentiation of pituitary corticotrope-derived AtT20 D16V cells

상세보기
J. Orthop. Res. Santos 27 1280 2009 10.1002/jor.20888 Pulsating fluid flow modulates gene expression of proteins involved in Wnt signaling pathways in osteocytes

상세보기
Cell. Signal. Jing 27 1772 2015 10.1016/j.cellsig.2015.05.018 Down-regulation of Wt1 activates Wnt/beta-catenin signaling through modulating endocytic route of LRP6 in podocyte dysfunction in vitro

상세보기
J. Biol. Chem. Gaur 280 33132 2005 10.1074/jbc.M500608200 Canonical WNT signaling promotes osteogenesis by directly stimulating Runx2 gene expression

상세보기
J. Cell. Physiol. Macsai 215 578 2008 10.1002/jcp.21342 Roles of Wnt signalling in bone growth, remodelling, skeletal disorders and fracture repair

상세보기
J. Clin. Invest. Krishnan 116 1202 2006 10.1172/JCI28551 Regulation of bone mass by Wnt signaling

상세보기
J. Biol. Chem. Ouko 279 26707 2004 10.1074/jbc.M402877200 Wnt11 signaling promotes proliferation, transformation, and migration of IEC6 intestinal epithelial cells

상세보기
Bone Zhang 52 145 2013 10.1016/j.bone.2012.09.029 Wnt/beta-catenin signaling activates bone morphogenetic protein 2 expression in osteoblasts

상세보기
J. Cell Biol. Greber 128 5 1995 10.1083/jcb.128.1.5 Depletion of calcium from the lumen of endoplasmic reticulum reversibly inhibits passive diffusion and signal-mediated transport into the nucleus

상세보기
Endocrinology Pang 154 283 2013 10.1210/en.2012-1772 Characterization, neurosteroid binding and brain distribution of human membrane progesterone receptors delta and {epsilon} (mPRdelta and mPR{epsilon}) and mPRdelta involvement in neurosteroid inhibition of apoptosis

상세보기
J. Bone Miner. Res. Jing 29 2250 2014 10.1002/jbmr.2260 Pulsed electromagnetic fields partially preserve bone mass, microarchitecture, and strength by promoting bone formation in hindlimb-suspended rats

상세보기
J. Orthop. Trauma Fredericks 14 93 2000 10.1097/00005131-200002000-00004 Effects of pulsed electromagnetic fields on bone healing in a rabbit tibial osteotomy model

상세보기
Cell Biol. Int. Takahashi 29 237 2005 10.1016/j.cellbi.2004.12.009 Prolonged Ca2+ transients in ATP-stimulated endothelial cells exposed to 50 Hz electric fields

상세보기
Science Perez-Terzic 273 1875 1996 10.1126/science.273.5283.1875 Conformational states of the nuclear pore complex induced by depletion of nuclear Ca2+ stores

상세보기
Oncogene Miller 18 7860 1999 10.1038/sj.onc.1203245 Mechanism and function of signal transduction by the Wnt/B-catenin and Wnt/Ca2+ pathways

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Pulsed electromagnetic field induces Ca2+-dependent osteoblastogenesis in C3H10T1/2 mesenchymal cells through the Wnt-Ca2+/Wnt-β-catenin signaling pathway

Abstract ▼ AI-Helper

주제어

참고문헌 (25)

이 논문을 인용한 문헌

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트