[논문]Profiling of Serum Metabolites Using MALDI-TOF and Triple-TOF Mass Spectrometry to Develop a Screen for Ovarian Cancer

Lee, Jun Hwa; Kim, Yun Hwan; Kim, Kyung-Hee; Cho, Jae Youl; Woo, Sang Myung; Yoo, Byong Chul; Kim, Seung Cheol

doi:10.4143/crt.2017.275

Profiling of Serum Metabolites Using MALDI-TOF and Triple-TOF Mass Spectrometry to Develop a Screen for Ovarian Cancer 원문보기

Cancer research and treatment : official journal of Korean Cancer Association, v.50 no.3, 2018년, pp.883 - 893

Lee, Jun Hwa (Biomarker Branch, Research Institute, National Cancer Center, Goyang, Korea) , Kim, Yun Hwan (Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Ewha Womans University Mokdong Hospital, Ewha Womans University School of Medicine, Seoul, Korea) , Kim, Kyung-Hee (Biomarker Branch, Research Institute, National Cancer Center, Goyang, Korea) , Cho, Jae Youl (Department of Genetic Engineering, Sungkyunkwan University, Suwon, Korea) , Woo, Sang Myung (Biomarker Branch, Research Institute, National Cancer Center, Goyang, Korea) , Yoo, Byong Chul (Biomarker Branch, Research Institute, National Cancer Center, Goyang, Korea) , Kim, Seung Cheol (Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Ewha Womans University Mokdong Hospital, Ewha Womans University School of Medicine, Seoul, Korea)

Abstract ▼ AI-Helper

PurposeWe sought to develop a matrix assisted laser desorption ionization-time of flight (MALDI-TOF)-based, ovarian cancer (OVC), low-mass-ion discriminant equation (LOME) and to evaluate a possible supportive role for triple-TOF mass analysis in identifying metabolic biomarkers.Materials and MethodsA total of 114 serum samples from patients with OVC and benign ovarian tumors were subjected to MALDI-TOF analysis and a total of 137 serum samples from healthy female individuals and patients with OVC, colorectal cancer, hepatobiliary cancer, and pancreatic cancer were subjected to triple-TOF analysis. An OVC LOME was constructed by reference to the peak intensity ratios of discriminatory low-mass ion (LMI) pairs. Triple-TOF analysiswas used to select and identify metabolic biomarkers for OVC screening.ResultsThree OVC LOMEs were finally constructed using discriminatory LMI pairs (137.1690 and 84.4119 m/z; 496.5022 and 709.7642 m/z; and 524.5614 and 709.7642 m/z); all afforded accuracies of > 90%. The LMIs at 496.5022 m/z and 524.5614 m/z were those of lysophosphatidylcholine (LPC) 16:0 and LPC 18:0. Triple-TOF analysis selected seven discriminative LMIs; each LMI had a specificity > 90%. Of the seven LMIs, fourwith a 137.0455 m/z ion atretention times of 2.04-3.14 minuteswere upregulated in sera from OVC patients; the ion was identified as that derived from hypoxanthine.ConclusionMALDI-TOF–based OVC LOMEs combined with triple-TOF–based OVC metabolic biomarkers allow reliable OVC screening; the techniques are mutually complementary both quantitatively and qualitatively.

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참고문헌 (12)

1 Kim YH Kim SC Recent advances in the biomarkers for epithelial ovarian cancer J Gynecol Oncol 2011 22 219 21 22247797

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2 Menon U Gentry-Maharaj A Hallett R Ryan A Burnell M Sharma A Sensitivity and specificity of multimodal and ultrasound screening for ovarian cancer, and stage distribution of detected cancers: results of the prevalence screen of the UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS) Lancet Oncol 2009 10 327 40 19282241
3 Jacobs IJ Menon U Ryan A Gentry-Maharaj A Burnell M Kalsi JK Ovarian cancer screening and mortality in the UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS): a randomised controlled trial Lancet 2016 387 945 56 26707054
4 Buys SS Partridge E Black A Johnson CC Lamerato L Isaacs C Effect of screening on ovarian cancer mortality: the Prostate, Lung, Colorectal and Ovarian (PLCO) Cancer Screening Randomized Controlled Trial JAMA 2011 305 2295 303 21642681

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5 Lee JH Yoo BC Kim YH Ahn SA Yeo SG Cho JY Lowmass-ion discriminant equation (LOME) for ovarian cancer screening BioData Min 2016 9 32 27752286

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6 Lee JH Kim KH Park JW Chang HJ Kim BC Kim SY Low-mass-ion discriminant equation: a new concept for colorectal cancer screening Int J Cancer 2014 134 1844 53 24096867

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7 Kim K Yeo SG Yoo BC Identification of hypoxanthine and phosphoenolpyruvic acid as serum markers of chemoradiotherapy response in locally advanced rectal cancer Cancer Res Treat 2015 47 78 89 25143052

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8 Yoo BC Kong SY Jang SG Kim KH Ahn SA Park WS Identification of hypoxanthine as a urine marker for non-Hodgkin lymphoma by low-mass-ion profiling BMC Cancer 2010 10 55 20175931

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9 Lorenzi M Vannoni D Leoncini R Caldarone R Marinello E The determination of urinary oxypurines as markers of gastrointestinal tumors Tumori 1987 73 289 94 3603725

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10 Jung J Jung Y Bang EJ Cho SI Jang YJ Kwak JM Noninvasive diagnosis and evaluation of curative surgery for gastric cancer by using NMR-based metabolomic profiling Ann Surg Oncol 2014 21 Suppl 4 S736 42 25092158
11 Kyriakides M Rama N Sidhu J Gabra H Keun HC El-Bahrawy M Metabonomic analysis of ovarian tumour cyst fluid by proton nuclear magnetic resonance spectroscopy Oncotarget 2016 7 7216 26 26769844

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12 Chan TC Howell SB Role of hypoxanthine and thymidine in determining methotrexate plus dipyridamole cytotoxicity Eur J Cancer 1990 26 907 11 2171601

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Profiling of Serum Metabolites Using MALDI-TOF and Triple-TOF Mass Spectrometry to Develop a Screen for Ovarian Cancer 원문보기

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Profiling of Serum Metabolites Using MALDI-TOF and Triple-TOF Mass Spectrometry to Develop a Screen for Ovarian Cancer 원문보기

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