[논문]The Galleria mellonella Hologenome Supports Microbiota-Independent Metabolism of Long-Chain Hydrocarbon Beeswax

Kong, Hyun Gi; Kim, Hyun Ho; Chung, Joon-hui; Jun, JeHoon; Lee, Soohyun; Kim, Hak-Min; Jeon, Sungwon; Park, Seung Gu; Bhak, Jong; Ryu, Choong-Min

doi:10.1016/j.celrep.2019.02.018

[해외논문] The Galleria mellonella Hologenome Supports Microbiota-Independent Metabolism of Long-Chain Hydrocarbon Beeswax 원문보기

Cell reports, v.26 no.9, 2019년, pp.2451 - 2464.e5

Kong, Hyun Gi (Molecular Phytobacteriology Laboratory, Infection Disease Research Center, KRIBB) , Kim, Hyun Ho (The Clinomics Institute, Ulsan National Institute of Science and Technology) , Chung, Joon-hui (Molecular Phytobacteriology Laboratory, Infection Disease Research Center, KRIBB) , Jun, JeHoon (The Clinomics Institute, Ulsan National Institute of Science and Technology) , Lee, Soohyun (Molecular Phytobacteriology Laboratory, Infection Disease Research Center, KRIBB) , Kim, Hak-Min (Biomedical Engineering, Ulsan National Institute of Science and Technology) , Jeon, Sungwon (Biomedical Engineering, Ulsan National Institute of Science and Technology) , Park, Seung Gu (Biomedical Engineering, Ulsan National Institute of Science and Technology) , Bhak, Jong (Biomedical Engineering, Ulsan National Institute of Science and Technology) , Ryu, Choong-Min (Molecula)

Abstract ▼ AI-Helper

Summary The greater wax moth, Galleria mellonella, degrades wax and plastic molecules. Despite much interest, the genetic basis of these hallmark traits remains poorly understood. Herein, we assembled high-quality genome and transcriptome data from G. mellonella to investigate long-chain hydrocarbon wax metabolism strategies. Specific carboxylesterase and lipase and fatty-acid-metabolism-related enzymes in the G. mellonella genome are transcriptionally regulated during feeding on beeswax. Strikingly, G. mellonella lacking intestinal microbiota successfully decomposes long-chain fatty acids following wax metabolism, although the intestinal microbiome performs a supplementary role in short-chain fatty acid degradation. Notably, final wax derivatives were detected by gas chromatography even in the absence of gut microbiota. Our findings provide insight into wax moth adaptation and may assist in the development of unique wax-degradation strategies with a similar metabolic approach for a plastic molecule polyethylene biodegradation using organisms without intestinal microbiota. Highlights The weight of G. mellonella larvae is maintained by feeding wax in germ-free conditions Intestinal beeswax metabolism is similar regardless of intestinal microbiota Genome and RNA sequencing provides insights into molecular mechanisms of wax degradation Polyethylene metabolism can occur independently of intestinal microbiota Graphical Abstract [DISPLAY OMISSION]

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