[논문]PEGylated liposome encapsulating nido-carborane showed significant tumor suppression in boron neutron capture therapy (BNCT)

Lee, Woonghee; Sarkar, Swarbhanu; Ahn, Heesu; Kim, Jung Young; Lee, Yong Jin; Chang, Yongmin; Yoo, Jeongsoo

doi:10.1016/j.bbrc.2019.11.144

PEGylated liposome encapsulating nido-carborane showed significant tumor suppression in boron neutron capture therapy (BNCT)

Biochemical and biophysical research communications, v.522 no.3, 2020년, pp.669 - 675

Lee, Woonghee (Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, School of Medicine, Kyungpook National University) , Sarkar, Swarbhanu (Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, School of Medicine, Kyungpook National University) , Ahn, Heesu (Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, School of Medicine, Kyungpook National University) , Kim, Jung Young (Division of Applied RI, Korea Institute of Radiological and Medical Sciences) , Lee, Yong Jin (Division of Applied RI, Korea Institute of Radiological and Medical Sciences) , Chang, Yongmin (Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, School of Medicine, Kyungpook National University) , Yoo, Jeongsoo (Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, School of Me)

Abstract ▼ AI-Helper

Abstract Boron neutron capture therapy (BNCT) is a binary radiotherapy based on nuclear reactions that occur when boron-10 is irradiated with neutrons, which result in the ejection of high-energy alpha particles. Successful BNCT requires the efficient delivery of a boron-containing compound to effect high concentrations in tumor cells while minimizing uptake in normal tissues. In this study, PEGylated liposomes were employed as boron carriers to maximize delivery to tumors and minimize uptake in the reticuloendothelial system (RES). The water-soluble potassium salt of nido-7,8-carborane, nido-carborane, was chosen as the boron source due to its high boron content per molecule. Nido-carborane was encapsulated in the aqueous cores of PEGylated liposomes by hydrating thin lipid films. Repeated freezing and thawing increased nido-carborane loading by up to 47.5 ± 3.1%. The average hydrodynamic diameter of the prepared boronated liposomes was determined to be 114.5 ± 28 nm through dynamic light scattering (DLS) measurement. Globular liposomes approximately 100 nm in diameter were clearly visible in transmission electron microscope (TEM) images. The viability of tumor cells following BNCT with 70 μM nido-carborane was reduced to 17.1% compared to irradiated control cells, which did not contain boronated liposomes. Confocal microscopy revealed that fluorescently labeled liposomes injected into the tail veins of mice were deeply and evenly distributed in tumor tissues and localized in the cytoplasm of tumor cells. When mice were properly shielded with a 12 mm-thick polyethylene board during in-vivo irradiation at a thermal neutron flux of 1.94 × 104/cm2·sec, almost complete tumor suppression was achieved in tumor models injected with boronated liposomes (21.0 mg 10B/kg). Two BNCT cycles spaced 10 days apart further enhanced the therapeutic anti-tumor effect, even when the dose was lowered to 10.5 mg 10B/kg. No notable weight loss was observed in the tumor models during the BNCT study. Highlights High concentrations of nido-7,8-carborane were efficiently encapsulated in PEGylated liposomes. Injected boronated liposomes penetrated tumor tissues deeply and were evenly distributed. Tumor growth was significantly suppressed in mice given BNCT versus control mice. Graphical abstract [DISPLAY OMISSION]

주제어

참고문헌 (24)

Angew. Chem. Int. Ed. Hawthorne 32 950 1993 10.1002/anie.199309501 The role of chemistry in the development of boron neutron-capture therapy of cancer

상세보기
Cancer Commun. Barth 38 35 2018 10.1186/s40880-018-0299-7 Boron delivery agents for neutron capture therapy of cancer

상세보기
Appl. Radiat. Isot. Barth 67 S3 2009 10.1016/j.apradiso.2009.03.102 Boron neutron capture therapy at the crossroads: challenges and opportunities

상세보기
Radiat. Oncol. Barth 7 146 2012 10.1186/1748-717X-7-146 Current status of boron neutron capture therapy of high grade gliomas and recurrent head and neck cancer

상세보기
J. Med. Chem. Altieri 52 7829 2009 10.1021/jm900763b Carborane derivatives loaded into liposomes as efficient delivery systems for boron neutron capture therapy

상세보기
P. Natl. Acad. Sci. USA. Feakes 92 1367 1995 10.1073/pnas.92.5.1367 Selective boron delivery to murine tumors by lipophilic species incorporated in the membranes of unilamellar liposomes

상세보기
P Natl Acad Sci USA Kueffer 110 6512 2013 10.1073/pnas.1303437110 Boron neutron capture therapy demonstrated in mice bearing EMT6 tumors following selective delivery of boron by rationally designed liposomes

상세보기
P Natl Acad Sci USA Shelly 89 9039 1992 10.1073/pnas.89.19.9039 Model studies directed toward the boron neutron-capture therapy of cancer - boron delivery to murine tumors with liposomes

상세보기
Biomaterials Gao 104 201 2016 10.1016/j.biomaterials.2016.06.046 Use of boron cluster-containing redox nanoparticles with ROS scavenging ability in boron neutron capture therapy to achieve high therapeutic efficiency and low adverse effects

상세보기
Chem. Commun. Tachikawa 50 12325 2014 10.1039/C4CC04344H Spermidinium closo-dodecaborate-encapsulating liposomes as efficient boron delivery vehicles for neutron capture therapy

상세보기
Transl. Oncol. Maitz 10 686 2017 10.1016/j.tranon.2017.05.003 Validation and comparison of the therapeutic efficacy of boron neutron capture therapy mediated by boron-rich liposomes in multiple murine tumor models

상세보기
Bioconjug. Chem. Koganei 24 124 2013 10.1021/bc300527n Development of high boron content liposomes and their promising antitumor effect for neutron capture therapy of cancers

상세보기
Chem. Rev. Scholz 111 7035 2011 10.1021/cr200038x Carbaboranes as pharmacophores: properties, synthesis, and application strategies

상세보기
Dalton Trans. Armstrong 4240 2007 10.1039/b709843j The bioinorganic and medicinal chemistry of carboranes: from new drug discovery to molecular imaging and therapy

상세보기
Adv. Drug Deliv. Rev. Fang 63 136 2011 10.1016/j.addr.2010.04.009 The EPR effect: unique features of tumor blood vessels for drug delivery, factors involved, and limitations and augmentation of the effect

상세보기
Adv. Drug Deliv. Rev. Maruyama 63 161 2011 10.1016/j.addr.2010.09.003 Intracellular targeting delivery of liposomal drugs to solid tumors based on EPR effects

상세보기
Acc. Chem. Res. Al-Jamal 44 1094 2011 10.1021/ar200105p Liposomes: from a clinically established drug delivery system to a nanoparticle platform for theranostic nanomedicine

상세보기
Colloids Surf., B Eloy 123 345 2014 10.1016/j.colsurfb.2014.09.029 Liposomes as carriers of hydrophilic small molecule drugs: strategies to enhance encapsulation and delivery

상세보기
Contrast Media Mol. Jung I 3727109 2018 Image-guided neutron capture therapy using the Gd-DO3A-BTA complex as a new combinatorial treatment approach
Inorg. Synth. Plesek 22 231 1983 10.1002/9780470132531.ch53 Potassium dodecahydro-7, 8-dicarba-nido-undecaborate(1-), K[7,8-C2B9H12], intermediates, stock solution, and anhydrous salt
ACS Med. Chem. Lett. Kim 5 390 2014 10.1021/ml400513g Vivid tumor imaging utilizing liposome-carried bimodal radiotracer

상세보기
Proc. Natl. Acad. Sci. U. S. A. Feakes 91 3029 1994 10.1073/pnas.91.8.3029 Na3[B20H17NH3]: synthesis and liposomal delivery to murine tumors

상세보기
Int. J. Pharm. Xu 423 410 2012 10.1016/j.ijpharm.2011.12.019 Predicting hydrophilic drug encapsulation inside unilamellar liposomes

상세보기
Compos. Sci. Technol. Zhang 150 16 2017 10.1016/j.compscitech.2017.06.007 Enhancing the neutron shielding ability of polyethylene composites with an alternating multi-layered structure

상세보기

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

PEGylated liposome encapsulating nido-carborane showed significant tumor suppression in boron neutron capture therapy (BNCT)

Abstract ▼ AI-Helper

주제어

참고문헌 (24)

이 논문을 인용한 문헌

연구과제 타임라인

관련 콘텐츠

원문 URL 링크

연관된 기능

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

PEGylated liposome encapsulating nido-carborane showed significant tumor suppression in boron neutron capture therapy (BNCT)

Abstract ▼ AI-Helper

주제어

참고문헌 (24)

이 논문을 인용한 문헌

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

관련 콘텐츠

원문 URL 링크

연관된 기능

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트