[논문]RNA-seq based transcriptome analysis of EHMT2 functions in breast cancer

Kim, Kwangho; Ryu, Tae Young; Ryu, Jea-Woon; Han, Tae-Su; Jung, Cho-Rok; Son, Mi-Young; Kim, Dae-Soo; Cho, Hyun-Soo

doi:10.1016/j.bbrc.2020.01.128

[해외논문] RNA-seq based transcriptome analysis of EHMT2 functions in breast cancer

Biochemical and biophysical research communications, v.524 no.3, 2020년, pp.672 - 676

Kim, Kwangho (Korea Research Institute of Bioscience and Biotechnology) , Ryu, Tae Young (Korea Research Institute of Bioscience and Biotechnology) , Ryu, Jea-Woon (Korea Research Institute of Bioscience and Biotechnology) , Han, Tae-Su (Korea Research Institute of Bioscience and Biotechnology) , Jung, Cho-Rok (Korea Research Institute of Bioscience and Biotechnology) , Son, Mi-Young (Korea Research Institute of Bioscience and Biotechnology) , Kim, Dae-Soo (Korea Research Institute of Bioscience and Biotechnology) , Cho, Hyun-Soo (Korea Research Institute of Bioscience and Biotechnology)

Abstract ▼ AI-Helper

Abstract For breast cancer treatment, hormone therapy is effective for hormone receptor-positive breast cancer but not for TNBC (triple-negative breast cancer). Thus, many researchers have attempted to identify more effective therapeutic candidates for all subtypes of breast cancer. In this study, we established an RNA-seq analytical pipeline to analyze the subtype-specific functions of EHMT2 in the MB231 and MCF7 cell lines. After EHMT2 knockdown, we identified subtype-specific DEGs (differentially expressed genes) and overlapping DEGs. Through GO (Gene Ontology) analysis, GSEA (gene set enrichment analysis), and KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis using the DEGs, we identified the subtype-specific functions of EHMT2 in the MB231 and MCF7 cell lines. Therefore, herein, we suggest that EHMT2 is an attractive therapeutic target for the treatment of all types of breast cancer. Highlights EHMT2 knockdown suppressed cell proliferation and migration/invasion in MB231 and MCF7. In RNA-seq analysis, EHMT2 have cell type specific functions in MB231 and MCF7. EHMT2 is an attractive therapeutic target for treating all types of breast cancer.

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