[논문]A rapid method for simultaneous quantification of berberine, berbamine, magnoflorine and berberrubine in mouse serum using UPLC-MS/MS

Liang, Xiufang; Xiang, Yunan; Li, Yanling; Feng, Ping; Qin, Yongping; Lai, Xianrong

doi:10.1016/j.jchromb.2020.122040

A rapid method for simultaneous quantification of berberine, berbamine, magnoflorine and berberrubine in mouse serum using UPLC-MS/MS

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, v.1142, 2020년, pp.122040 -

Liang, Xiufang (Institute of Clinical Trials, West China Hospital, Sichuan University) , Xiang, Yunan (Chengdu University of Traditional Chinese Medicine) , Li, Yanling (Chengdu University of Traditional Chinese Medicine) , Feng, Ping (Institute of Clinical Trials, West China Hospital, Sichuan University) , Qin, Yongping (Institute of Clinical Trials, West China Hospital, Sichuan University) , Lai, Xianrong (Chengdu University of Traditional Chinese Medicine)

Abstract ▼ AI-Helper

Abstract Berberidis cortex, the dry bark of Berberis L., is used to treat diabetes and contains at least three bioactive components: berberine (BBR), berbamine (BBM) and magnoflorine (MGF). BBR in turn is metabolized into berberrubine (BRB). Although it is possible to quantify each of these components individually in serum, there are currently no methods for simultaneously quantifying all four. Here, we developed a specific and rapid method for simultaneously quantifying BBR, BBM, MGF and BRB in mouse serum using ultra high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Samples were pretreated by protein precipitation, separated using an ACQUITY UPLC® BEH C18 column and detected by a triple quadrupole mass spectrometer with electrospray ionization. The compound [9,10-(OC2H3)2]-BBR (d 6-BBR) was used as internal standard for BBR and BRB, boldine (BOL) for MGF and tetrandrine (TET) for BBM. The m/z transitions for precursor/product ion pairs were 336.1/320.2 for BBR, 305.2/566.3 for BBM, 342.0/297.1 for MGF, 322.1/307.2 for BRB, 342.2/294.3 for d 6-BBR, 312.2/580.3 for TET and 328.1/265.2 for BOL. We validated our method in terms of selectivity, linearity and lower limit of quantification, accuracy, precision, matrix effect and recovery, dilution integrity and stability. This method showed good linearity from 0.1 to 40 ng/mL for BBR, 8 to 3200 ng/mL for BBM, 5 to 2000 ng/mL for MGF and 0.2 to 80 ng/mL for BRB. The chromatographic run time was 3.9 min, and sample preparation took approximately 15 min per batch. Finally, we used our method to measure BBR, BBM, MGF and BRB in serum from diabetic mice after gavage administration of BBR hydrochloride, BBM hydrochloride, and MGF. This method is precise, accurate and suitable for high-throughput sample analysis. Highlights A method to quantitate Berberis components and berberrubine is described. Adjusting collision energy gave good linearity for all analytes. One complete analysis required only 20 μL of mouse serum and 15 min.

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참고문헌 (33)

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A rapid method for simultaneous quantification of berberine, berbamine, magnoflorine and berberrubine in mouse serum using UPLC-MS/MS

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