[논문]Development of a Laboratory-safe and Low-cost Detection Protocol for SARS-CoV-2 of the Coronavirus Disease 2019 (COVID-19)

Won, Joungha; Lee, Solji; Park, Myungsun; Kim, Tai Young; Park, Mingu Gordon; Choi, Byung Yoon; Kim, Dongwan; Chang, Hyeshik; Kim, V. Narry; Lee, C. Justin

doi:10.5607/en20009

[국내논문] Development of a Laboratory-safe and Low-cost Detection Protocol for SARS-CoV-2 of the Coronavirus Disease 2019 (COVID-19) 원문보기

Experimental neurobiology : en, v.29 no.2, 2020년, pp.107 - 119

Won, Joungha (Center for Cognition and Sociality, Cognitive Glioscience Group, Institute for Basic Science, Daejeon 3426, Korea) , Lee, Solji (Center for Cognition and Sociality, Cognitive Glioscience Group, Institute for Basic Science, Daejeon 3426, Korea) , Park, Myungsun (Center for Cognition and Sociality, Cognitive Glioscience Group, Institute for Basic Science, Daejeon 3426, Korea) , Kim, Tai Young (Center for Cognition and Sociality, Cognitive Glioscience Group, Institute for Basic Science, Daejeon 3426, Korea) , Park, Mingu Gordon (Center for Cognition and Sociality, Cognitive Glioscience Group, Institute for Basic Science, Daejeon 3426, Korea) , Choi, Byung Yoon (Department of Otorhinolaryngology, Seoul National University Bundang Hospital, Seongnam 13620, Korea) , Kim, Dongwan (Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea) , Chang, Hyeshik (Center for RNA Research, Institute for Basic Science, Seoul) , Kim, V. Narry , Lee, C. Justin

Abstract ▼ AI-Helper

The severe acute respiratory coronavirus 2 (SARS-CoV-2), which emerged in December 2019 in Wuhan, China, has spread rapidly to over a dozen countries. Especially, the spike of case numbers in South Korea sparks pandemic worries. This virus is reported to spread mainly through person-to-person contact via respiratory droplets generated by coughing and sneezing, or possibly through surface contaminated by people coughing or sneezing on them. More critically, there have been reports about the possibility of this virus to transmit even before a virus-carrying person to show symptoms. Therefore, a low-cost, easy-access protocol for early detection of this virus is desperately needed. Here, we have established a real-time reverse-transcription PCR (rtPCR)-based assay protocol composed of easy specimen self-collection from a subject via pharyngeal swab, Trizol-based RNA purification, and SYBR Green-based rtPCR. This protocol shows an accuracy and sensitivity limit of 1-10 virus particles as we tested with a known lentivirus. The cost for each sample is estimated to be less than 15 US dollars. Overall time it takes for an entire protocol is estimated to be less than 4 hours. We propose a cost-effective, quick-and-easy method for early detection of SARS-CoV-2 at any conventional Biosafety Level II laboratories that are equipped with a rtPCR machine. Our newly developed protocol should be helpful for a first-hand screening of the asymptomatic virus-carriers for further prevention of transmission and early intervention and treatment for the rapidly propagating virus.

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참고문헌 (18)

1 Wang D Hu B Hu C Zhu F Liu X Zhang J Wang B Xiang H Cheng Z Xiong Y Zhao Y Li Y Wang X Peng Z 2020 Clinical characteristics of 138 hospitalized patients with 2019 novel Coronavirus-infected pneumonia in Wuhan, China JAMA doi: 10.1001/jama.2020.1585 10.1001/jama.2020.1585 32031570

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2 Centers for Disease Control and Prevention 2020 How COVID-19 spreads [Internet] Centers for Disease Control Atlanta Available from: https://www.cdc.gov/coronavirus/2019-ncov/about/transmission.html
3 Bendix A 2020 A person can carry and transmit COVID-19 without showing symptoms, scientists confirm [Internet] Business Insider New York Available from: https://www.sciencealert.com/researchers-confirmed-patients-can-transmit-the-coronavirus-without-showing-symptoms
4 Hoehl S Berger A Kortenbusch M Cinatl J Bojkova D Rabenau H Behrens P Boddinghaus B Gotsch U Naujoks F Neumann P Schork J Tiarks-Jungk P Walczok A Eickmann M Vehreschild MJGT Kann G Wolf T Gottschalk R Ciesek S 2020 Evidence of SARS-CoV-2 infection in returning travelers from Wuhan, China N Engl J Med doi: 10.1056/NEJMc2001899 10.1056/NEJMc2001899 32069388

상세보기
5 Yu P Zhu J Zhang Z Han Y Huang L 2020 A familial cluster of infection associated with the 2019 novel coronavirus indicating potential person-to-person transmission during the incubation period J Infect Dis doi: 10.1093/infdis/jiaa077 10.1093/infdis/jiaa077 32067043

상세보기
6 Ministry of Health and Welfare 2020 [Government support 2019 - novel coronavirus testing condition] South Korea Sejong Korean
7 Ahn S 2020 [Coronavirus test cost] [Internet] LawTalk News Seoul Available from: https://news.lawtalk.co.kr/issues/1848 . Korean
8 Yu E 2020 [Lack of medical personnel following the corona spread] [Internet] dongA.com Seoul Available from: http://www.donga.com/news/article/all/20200221/99817769/1 . Korean
9 Corman VM Landt O Kaiser M Molenkamp R Meijer A Chu DKW Bleicker T Brunink S Schneider J Schmidt ML Mulders DGJC Haagmans BL van der Veer B van den Brink S Wijsman L Goderski G Romette JL Ellis J Zambon M Peiris M Goossens H Reusken C Koopmans MPG Drosten C 2020 Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR Euro Surveill doi: 10.2807/1560-7917.ES.2020.25.3.2000045 10.2807/1560-7917.ES.2020.25.3.2000045 31992387

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10 Blow JA Dohm DJ Negley DL Mores CN 2004 Virus inactivation by nucleic acid extraction reagents J Virol Methods 119 195 198 10.1016/j.jviromet.2004.03.015 15158603

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11 Centers for Disease Control and Prevention 2020 Research use only 2019-novel Coronavirus (2019-nCoV) real-time RT-PCR primer and probe information [Internet] Centers for Disease Control and Prevention Atlanta Available from: https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html
12 Park WB Kwon NJ Choi SJ Kang CK Choe PG Kim JY Yun J Lee GW Seong MW Kim NJ Seo JS Oh MD 2020 Virus isolation from the first patient with SARS-CoV-2 in Korea J Korean Med Sci 35 e84 10.3346/jkms.2020.35.e84 32080990

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13 Centers for Disease Control and Prevention 2020 Interim laboratory biosafety guidelines for handling and processing specimens associated with Coronavirus disease 2019 (COVID-19) [Internet] Centers for Disease Control and Atlanta Available from: https://www.cdc.gov/coronavirus/2019-ncov/lab/lab-biosafety-guidelines.html
14 Murray MA Schulz LA Furst JW Homme JH Jenkins SM Uhl JR Patel R Cockerill FC Myers JF Pritt BS 2015 Equal performance of self-collected and health care worker-collected pharyngeal swabs for group a streptococcus testing by PCR J Clin Microbiol 53 573 578 10.1128/JCM.02500-14 25502528

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15 Ventura A Meissner A Dillon CP McManus M Sharp PA Van Parijs L Jaenisch R Jacks T 2004 Cre-lox-regulated conditional RNA interference from transgenes Proc Natl Acad Sci U S A 101 10380 10385 10.1073/pnas.0403954101 15240889

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16 Thermo Fisher Scientific 2020 The manual of TRIzol TM LS Reagent [Internet] Thermo Fisher Scientific Waltham Available from: https://www.thermofisher.com/order/catalog/product/10296028
17 Chu DKW Pan Y Cheng SMS Hui KPY Krishnan P Liu Y Ng DYM Wan CKC Yang P Wang Q Peiris M Poon LLM 2020 Molecular diagnosis of a novel Coronavirus (2019-nCoV) causing an outbreak of pneumonia Clin Chem doi: 10.1093/clinchem/hvaa029 10.1093/clinchem/hvaa029 32031583

상세보기
18 Gopalan V Vioque A Altman S 2002 RNase P: variations and uses J Biol Chem 277 6759 6762 10.1074/jbc.R100067200 11741968

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[국내논문] Development of a Laboratory-safe and Low-cost Detection Protocol for SARS-CoV-2 of the Coronavirus Disease 2019 (COVID-19) 원문보기

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[국내논문] Development of a Laboratory-safe and Low-cost Detection Protocol for SARS-CoV-2 of the Coronavirus Disease 2019 (COVID-19) 원문보기

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