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| 국가/구분 | United States(US) Patent 등록 |
|---|---|
| 국제특허분류(IPC7판) |
|
| 출원번호 | US-0693164 (2010-01-25) |
| 등록번호 | US-8753668 (2014-06-17) |
| 발명자 / 주소 |
|
| 출원인 / 주소 |
|
| 대리인 / 주소 |
|
| 인용정보 | 피인용 횟수 : 2 인용 특허 : 378 |
Disclosed are methods for producing yeast β-glucan and mannan preparations. The methods employ an autolysis process, followed by enzymatic treatment with one or more of a protease, glucanase or lipase. The preparations produced may be used in food supplements, pharmaceuticals, cosmetics, animal feed
Disclosed are methods for producing yeast β-glucan and mannan preparations. The methods employ an autolysis process, followed by enzymatic treatment with one or more of a protease, glucanase or lipase. The preparations produced may be used in food supplements, pharmaceuticals, cosmetics, animal feeds, and neutraceuticals.
1. A method for processing yeast cells comprising: (a) autolyzing the yeast cells at a temperature of 50° C. to 65° C. to release yeast cell walls;(b) incubating the cell walls with an exogenous protease at a pH of 9 to 10;(c) incubating the protease-treated cell walls of step (b) with an enzyme com
1. A method for processing yeast cells comprising: (a) autolyzing the yeast cells at a temperature of 50° C. to 65° C. to release yeast cell walls;(b) incubating the cell walls with an exogenous protease at a pH of 9 to 10;(c) incubating the protease-treated cell walls of step (b) with an enzyme comprising at least one of an amylase, lipase and a combination thereof;(d) separating the enzyme-treated cell walls of step (c) into a glucan-enriched component and a mannan-enriched component; and(e) separately retaining said glucan-enriched component and said mannan-enriched component;wherein said glucan-enriched component of step (e) can be added to an animal feed or to a product selected from a food supplement, pharmaceutical, cosmetic and neutraceutical. 2. The method of claim 1, wherein the yeast cells comprise brewer's yeast cells. 3. The method of claim 1, wherein step (c) is carried out at a pH of 4 to 6. 4. The method of claim 1, further comprising adding the glucan-enriched component of step (e) to a product selected from a food supplement, pharmaceutical, cosmetic and neutraceutical. 5. The method of claim 1, further comprising (f) ultrafiltering the mannan-enriched component of step (e) to form a filtrate and a retentate. 6. The method of claim 1, wherein the retentate comprises mannans, and wherein at least 85% (w/w) of the mannans have a molecular weight of at least 10,000 Da. 7. The method of claim 5, further comprising adding the filtrate of step (f) to a product selected from a food supplement, pharmaceutical, cosmetic and neutraceutical.
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