초록 ▼

가. Infusion pump를 이용한 미정맥 연속투여 시험법 확립 미정맥 연속투여를 실시함에 있어서 필요한 제반 기술확립과 생리식염수 투여시험나. 정충독성 시험법을 확립 수태능력 평가를 위해서 필요한 제반기술 확립과 항암제 투여시험다. 화학물질의 신경독성 평가방법 확립 약물의 신경독성작용에 대한 검색체계확립 및 FOB 시험라. 단기발암성 시험방법 약물의 발암성검색의 경제화와 간장에서 전암병변검색마. 트란스지닉 마우스를 이용한 돌연변이 시험법 확립 본 연구기간에는 Stratagene 사의 Big BlueTM system을 이용하는

가. Infusion pump를 이용한 미정맥 연속투여 시험법 확립 미정맥 연속투여를 실시함에 있어서 필요한 제반 기술확립과 생리식염수 투여시험나. 정충독성 시험법을 확립 수태능력 평가를 위해서 필요한 제반기술 확립과 항암제 투여시험다. 화학물질의 신경독성 평가방법 확립 약물의 신경독성작용에 대한 검색체계확립 및 FOB 시험라. 단기발암성 시험방법 약물의 발암성검색의 경제화와 간장에서 전암병변검색마. 트란스지닉 마우스를 이용한 돌연변이 시험법 확립 본 연구기간에는 Stratagene 사의 Big BlueTM system을 이용하는 in vivo 돌연변이 시험법을 확립하고자 하였다.바. 환경독성 연구 화학물질의 토양대사시험과 어류생육초기독성시험법 개발 및 살충제 독성시험사. 외국공인 획득을 위한 GLP 시스템 구축 안전성평가기술의 GLP 시스템구축과 환경독성평가 기술의 GLP 시스템구축:모의사찰 및 SOP 정리아. 신의약·신농약 지원연구 신의약 및 신농약 개발 연구자. 미생물 모니터링 기술확립 SPF 실험동물 유지 및 미생물검사 시스템 구축차. 유전적 모니터링 기술확립 SPF 실험동물의 육종 및 누드마우스 생산을 위한 기초자료 조사카. 표준화된 SPF ICR 마우스의 육종기술 연구 실험동물 생산과 육종기술 연구 확립타. 실험동물 Database 확립 C59BL/6 마우스의 체중, 장기중량, 혈액학적치, 혈액생화학치의 분석과 연령별 변동상황파. Nude 마우스 육종을 위한 기초연구 Nude 마우스의 사육조건과 육종체계 확립을 위한 기초조사하. 실험동물 대량생산 보급 G7 과제의 약물검색과 안전성평가를 위한 생산체계 확립

Abstract ▼

A. A study on continuous intravenous injection by using infusion pump in rats To establish an i.v. injection method by using infusion pumps in rats, which is necessary in toxicity tests. Physiological saline was administered through the tail vein of the rat by using infusion pumps for 1 week to

A. A study on continuous intravenous injection by using infusion pump in rats To establish an i.v. injection method by using infusion pumps in rats, which is necessary in toxicity tests. Physiological saline was administered through the tail vein of the rat by using infusion pumps for 1 week to determine the appropriate infusion volume and speed which will be applied to other test substances.B. A study on the Sperm Evaluations Sperm evaluation methods were established in rat fertility test of DA-125, an anticancer antibiotic, in order to make a efficient evaluation of drug-induced infertility. Fertility index, weights of testis and epididymis, histopathological examination of testis were investigated in rat fertility test of DA-125. Ih additian, spermatid number in testis, daily sperm production rate, sperm number in cauda epididymis, sperm morphology, motility, velocity and serum testosteron concentration were measured.C. Assessment and Evaluation of the Neurotoxicity Test, the Functional Observational Battery (FOB) Central nervous system(CNS) toxicity historically been defined by histopathological, electrophysiological or neurochemical techniques. The effects of chemicals on the functional integrity of the CNS have more recently been studied utilizing techniques which measure the integrated behavior of organisms. The use of multiple behavioral methodologies for detecting neurobehavioral toxicity evolved from early work in phamacology, since then, a variety of behavioral test batteries have been described for assessing neurotoxic potential of exogenous agents. The office of toxic substances of the U.S. EPA has prepared guidelines using the behaviroal methodologies for testing for chemical-induced neurotoxicity (U.S. EPA. 1985). These guidelines include the following behavioral tests ; a functional observational battery(FOB), motor activity and schedule-controlled behavior. FDA now recognizes that information about neurotoxicity is significant endpoint in assessing the safety of chemicals and published testing guidelines described the use of a functional observational battery. In response to the recent regulatory activities in this area, a battery of behavioral tests has been developed in this laboratory to screen for potential neurotoxicity of industrial chemicals, pesticides and drugs and the FOB protocol(standard operating procedure), used in the present experiment was based lagely on the EPA test guidelines, but with some modifications.D. Medium-term carcinogenicity bioassay Several chemicals were tested for carcinogenicity by a new medium-term carcinogenicity bioassay using D-galactosamine.E. Establishment of Transgenic mouse mutagenesis assay system It was intended to introduce transgenic mouse mutagenesis assay system(Big Blue TM) to this laboratory this year for in in vivo mutagenicity test.F. Study on the environmental toxicology (1) Establishment of method for anaerobic soil metabolism study of chemicals (2) Establishment of method for fish early-life toxicity test (3) Establishment of GLP system in environmental toxicological testing of chemicalsG. Construction of the system for GLP(Good Laboratory Practice) accrediation in advanced nations (1) Preparation for the accrediation of AAALAC(The American Association for the Accrediation of Laboratory Animal Care) (2) Preparation of KRICT TRC-GLP in English (3) Maintenance of GLP accrediation from Japanese Ministry of Agriculture, Forestry and FisheriesH. Establishment of the microbiological monitoring technique The microbiological monitoring technique was established and the experimental animals breeded in our facilities and the breeding environment were checked by the technique to maintain the animals as specific pathgen free.I. Establishment of genetic monitoring technique These studies were carried out to establish the genetic monitoring system for inbred mice through the detection of biochemical genetic markers with Helena system (Helena Lab., USA) and modified ATTO system (ATTO Corporation, Japan) of Titan III. Six loci of biochemical markers, mainly critical subset, were tested.J. A study on the breeding technique of the standardized SPF Ktc:ICR mouse for high quality The number of suckling litters of ICR mice was fixed as 10, 12, 14 and 16 to establish the appropriate number of suckling litter for supply of animals which were equally developed. The changes of body weight from 0 to 4 weeks after the birth, the weaning rate and the ratio of the undergrowth at 3 and 4 weeks old were investigated.K. Studies on the basic data of SPF Ktc:C57BL/6 mice Basic study of Ktc:C57BL/6 mice breeded in our facilities were collected. The body weight and organ weights were measured and the biochemical tests of urine and blood were carried out at 10, 15 and 18 weeks old.L. Basic study on the breeding technique of the nude mice Basic dondition for breeding the nude mice was investigated and the facilities for breeding the nude mice were designed.M: Mass production and the supply of the experimental animals The experimental animals were mass produced through the single paired rotational mating system and supplied.

목차 Contents

• 제1장 서론...29
• 제2장 과제구성...31
• 제3장 세부과제별 연구결과...35
• 제4장 결론...39
• 제5장 향후 연구계획...41