초록

1. 미곡산물의 혈압강하 기능소재 탐색 및 개발 미강단백질 가수분해물로부터 Angiotensin converting enzyme 저해효과를 확인하며, 그 저해 peptide 구조규명을 위하여 분리 정제방법을 확립하고, peptide분획물의 본태성고혈압쥐에 대한 혈압강하 효과를 확인한다.2. 미곡부산물의 혈당강하 기능소재 탐색 및 개발 α-amylase와 α-glucohydrolases저해 시험방법을 확립하며, 미강단백질 및 ethanol추출물의 이들 효소에 대한 저해효과를 확인한다.

Abstract ▼

Ⅰ. Title of Research A Study on Development of Hypotensive and Hypoglycemic Functional Material of Rice Byproducts Ⅱ. Objective ana Importance of Research The objectives of this research were to develop essential technology to prepare functional foods from hypotensive and hypoglycemic components o

Ⅰ. Title of Research A Study on Development of Hypotensive and Hypoglycemic Functional Material of Rice Byproducts Ⅱ. Objective ana Importance of Research The objectives of this research were to develop essential technology to prepare functional foods from hypotensive and hypoglycemic components of rice byproducts. The research is important in that prevention and treatment of diseases by utilizing specific food functional components is more efficient than medical curement after onset of diseases Ⅲ. Scope and Contents of Research1. Development of Hypotensive Functional Material of Rice Byproducts Determine angiotensin converting enzyme (ACE) inhibitory activities of rice bran protein hydrolysate, set up the separation ana purification process of ACE inhibitory peptide and prove in vivo hypotensive effect of the separated fraction.2. Development of Hypoglycemic Functional Material of Rice Byproducts Set up the method of a-amylase and a-glucohydrolases inhibition and determine the inhibitory effects of rice bran protein and ethanol extractIV Conclusion and Recommendation1. Development of Hypotensive Functional Material from Rice Byproducts ACE inhibitory activity of rice bran was investigated. Isolated rice bran protein was hydrolyBed with each of pepsin, Novozym 89L, Neutrase 0.5L and Alcalase 0.6L at each pH of 2.0, 5.0, 6.5 and 8.0 for 3 hours, respectively. Pepsin was most effective on peptide production and ACE inhibitory ability among other enzymes, The low molecular weight fraction (MW < 1,000) was more inhibitory on ACE than the high molecular weight portion (MW > 1,000) of each enzymatic hydrolysate The low molecular weight portion of peptic hydrolysate was partially purified by successive column chromatography of SP Sephadex C-25, ODS AQ-1205 and a reverse-phase HPLC with YMC-Pack ODS-l2OA column. When the fraction eluted from SP Sephadex C-25 column was orally administered to the spontanously hypertensive rats, the blood pressure was siginificantly reduced 3, 5 and 7 hours after administration. From these results it is possible to prepare hypotensive functional food from rice bran protein hyarolysate. 2. Development of Hypoglycemic Functional Material from Rice Byproducts It was investigated on the a-amylase and a-glucohydrolases inhibitory activity of rice bran. Rice bran protein (ammonium sulfate 40-60% saturation) and ethanol-extract showed inhibitory activity on α-amylase and α-glucohydrolase. The ethanol extract was partitioned into hexane, chloroform, ethyl acetate and water-soluble portions. Only the ethly acetate fraction had the inhibitory activities. This fraction was ohrornatographed on a silica gelcolumn, and successively eluted with cilloroform-methanol gradint. The fraction eluted with chloroform : methanol = 3:1 (v/v) showed the highest inhibitory activity among other fractions. Erom these results it is possible to prepare hypoglycemic functional food fromrice bran protein and ethanol extract after the in vivo effect and the active principle is confirmed

목차 Contents

• 표지 ...1
• 제출문 ...2
• 요약문 ...3
• 목차 ...12
• 제 1 장 서 론...15
• 제1절 연구개발의 목적과 범위...16
• 제2절 연구개발의 필요성...16
• 제3절 지금까지의 연구개발 실적...17
• 참고문헌...19
• 제 2 장 미곡부산물의 혈압강하 기능소재 탐색 및 개발...20
• 제1절 서설...22
• 제2절 재료 및 방법...27
• 1. 시 료...27
• 2. ACE 저해활성의 측정...27
• 3. Peptide 량 측정법...27
• 4. 미강 및 탈지미강의 단백질 분리...28
• 5. ACE 저해 peptide 의 최적제조조건검토...30
• 6. ACE 저해 peptide 분리, 정제...30
• 7. in vivo test 에 의한 강압효과 확인...31
• 제3절 결과 및 고찰...32
• 1. ACE 저해 peptide의 최적제조조건검토...32
• 1) 미강 및 탈지미강 단백질의 가수분해조건검토...32
• 2) Small peptide 및 large peptide fraction의 ACE 저해효과...34
• 2. ACE 저해 peptide 분리, 정제...36
• 1) SP Sephadex C-25 column chromatography...36
• 2) ODS AQ-12OS column chromatography...38
• 3) YMC-Pack ODS-12OA column을 이용한 HPLC...39
• 3. in vivo test 에 의한 강압효과 확인...47
• 제4절 결론...50
• 참고문헌...52
• 제 3 장 미곡부산물의 혈당강하 기능소재 탐색 및 개발...55
• 제1절 서설...56
• 제2절 재료 및 방법...60
• 1. 실험재료...60
• 가. 미 강...60
• 나. 시 약...60
• 2. 실험방법...60
• 가. α-amylase 및 α -glucohydrolases 저해 활성의 측정...60
• 1) α-amylase 저해 활성의 측정...60
• 2) α -glucohydrolases 저해 활성의 측정...61
• 나. 미강 추출물의 제조...61
• 1) 미강단백질의 추출...61
• 2) 미강의 ethanol 추출 및 분획...62
• 제3절 결과 및 고찰...64
• 1. α-amylase 및 α -glucohydrolases 저해활성...64
• 가. α-amylase 저해활성...64
• 나. α -glucohydrolases 저해활성...65
• 2. 미강단백질의 저해효과...67
• 가. 미강단백질의 α-amylase 저해효과...67
• 나. 미강단백질의 α -glucohydrolases 저해효과...68
• 3. 미강 ethanol 추출물의 저해효과...69
• 가. 미강 ethanol 추출물 및 그 분획물의 α-amylase 저해효과...69
• 나. Silica gel column 분획물의 α-amylase 저해효과...70
• 다. 미강ethanol추출물 및 그 분획물의 α-glucohydrolases 저해효과...71
• 라. Silica gel column 분획물의 α-glucohydrolases 저해효과...73
• 제4절 결론...78
• 참고문헌...79