초록

○ 유용향료성분 생산균주의 screening○ 선정균주의 방향성화합물의 분석 및 동정○ 향료성분 생성을 위한 적정배양조건조사

Abstract ▼

Title : Screening of flavor producing microorganismsAbstract : For the purpose of aroma production by microorganisms, we isolated 6 strains of aroma producing microorganisms from 108 soil samples. One of the strain named AY 70-4 which produced powerful apple-like aroma in potato dextrose broth mediu

Title : Screening of flavor producing microorganismsAbstract : For the purpose of aroma production by microorganisms, we isolated 6 strains of aroma producing microorganisms from 108 soil samples. One of the strain named AY 70-4 which produced powerful apple-like aroma in potato dextrose broth medium was further investigated. The strain AY 70-4 was tentatively identified as Geotrichum sp, based on its morphological and cultural characteristics; formed white and cottony colony, true septated hyphae and arthrospores without conidiophores on the potato dextrose agar medium after 3 days at 25℃. The apple-like odor of the Geotrichum sp. was most intense after 48 hours of shaking culture(40rpm) around pH 4.0-5.0. Volatile compounds of the culture broth were analyzed with high-resolution packed and open tubula capillary gas chromatographic columns and mass spectrometry. Thirty one of the forty four peaks on chromatograms could be identified: 18 esters, 6 free fatty acids, 4 alcohols, 2 aldehydes and 1 hydrocarbon. Twenty-four compounds of them are frequently found in apple flavor components. Especially, ethyl 2-methylpropionate, ethyl butyrate and ethyl propionate seem to play an important role in the of apple-like odor of Geotrichum sp. culture broth.

목차 Contents

• 제1장 서 론...17
• 제2장 재료 및 방법...19
• 제1절 실험재료...19
• 제2절 방 법...19
• 1. 방향성 화합물생성 미생물의 분리...19
• 2. 선발균주의 동정...20
• 가. 형태적 및 배양적 특성...20
• 나. 생리적 특성...21
• 3. Geotrichum sp. 의 방향생성을 위한 배양조건 검토...22
• 4. Geotrichum sp.가 생성하는 방향성분의 분석...23
• 가. Headspace GC...23
• 나. Caillary GC 및 GC-MS...24
• 제3장 결과 및 고찰...28
• 제1절 방향성 화합물생성 미생물의 분리...28
• 1. 시료의 채취...28
• 2. 분 리...28
• 제2절 AY70-4균주의 동정...32
• 제3절 Geotrichum sp.의 방향생성조건...38
• 1. pH의 영향...38
• 2. 진탕속도의 영향...39
• 제4절 Geotrichum sp.가 생성하는 방향성화합물의 분석...40
• 제4장 결론 및 건의사항...50
• 참고문헌...52