초록

O 부자 및 가공부자로부터 alkaloid성분(aconitine, hypaconitine, mesaconitine 등 및 benzoylaconine 등)의 분리
O 승마로부터 27-deoxyactein, deoxyacetylacteol 등의 분리
O 당귀로부터 decursin 및 기타 쿠마린 성분들의 분리
O 각 표준품의 순도 분석(각 표준품 100mg씩 제출 및 성적서 작성)
O 이들 생약 10kg 이상에서 얻을 수 있는 성분들을 달리하여 제출
O 기원이 명확한 대상생약의 확보(10-25g씩 제출)

Abstract ▼

The 7th Edition of the Korean Pharmacopoea describes about 530 crude drugs. However, only a limited number of the authentic standard compounds for evaluation of these crude drugs are described in this Pharmacopoea. To isolate these compounds from four crude drugs such as Aconiti Tuber, processed Aco

The 7th Edition of the Korean Pharmacopoea describes about 530 crude drugs. However, only a limited number of the authentic standard compounds for evaluation of these crude drugs are described in this Pharmacopoea. To isolate these compounds from four crude drugs such as Aconiti Tuber, processed Aconiti Tuber, Cimicifugae Rhizoma and Angelicaegigantis Radix, this work has been carried out. The known diterpene alkaloids such as neoline, lipohypaconitine from Aconiti Tuber as well as songorine and neoline from the processed Aconiti Tuber were isolated and identified. In addition , fournovel diterpene alkaloids, lipo 14-O-anisoyl bikhaconine and 14-cinnamoylneoline from Aconiti Tuber as well as 15-episongorine and deethylneoline from the processed Aconiti Tuber, were also isolated and characterized by means ofphysico-chemical and spectroscopic methods. However, the well-known diterpene alkaloids, aconitine, mesaconitine and hypaconitine, could not be detected form Aconiti Tuber. To isolate these compounds, the roots of Aconitum jaluense collected at Mt. Taebak area, Kangwon province were conducted, and we isolated five alkaloids including mesaconitine, hypaconitine, lipohypaconitine and neoline. To obtain benzoylaconine, aconitine purchased from Sigma Co. was refluxed in 50% dioxane solution for 1 hr at 120℃. The crude product was purified by silica gel column chromatography to give benzoylaconine. From Cimicifugae Rhizoma, saponins such as cimiside E, 24-epi-7,8-didehydrocimigenol 3-O-$\beta$-D-xyloside,23-O-acetylshengmanol 3-O-$\beta$-D-xyloside, cimigenol 3-O-$\beta$-D-xyloside and a mixture of cimiside C and D togetherwith a phenylpropanoid, isoferulic acid were isolated and characterized. The plant material of Cimicifuga dahurica from Mt. Gyebangarea, was also investigated, and we isolated two saponins, cimiside E and 23-O-acetylshengmanol 3-O-$\beta$-D-xyloside. It isimpossible to isolate the target compounds, 27-deoxyactein and deoxyacetylacteol from a couple of Cimicifuga extracts. Threecoumarins such as decursin, decursinol angelate and nodakenin were isolated from Angelica gigantis Radix. It is noteworthythat the results from these experiments on Aconiti Tuber and processed Aconiti Tuber were quite different from thosedescribed in the published papers.

목차 Contents

• 표지...1
• 제출문...3
• 요약문...5
• SUMMARY...7
• 목차...9
• 제1장 서론...10
• 제2장 국내ㆍ외 기술개발 현황...11
• 제3장 연구개발 수행내용 및 결과...17
• 부자의 성분연구 및 지표성분 분리...17
• 제1절 실험방법...17
• 제2절 투구꽃(Acontium jaluense Kom.)으로부터 alkaloid의 분리...23
• 제3절 결과 및 고찰...29
• 3.1. 화합물 1 (Neoline)...29
• 3.2. 화합물 2 (Lipohypaconitine)...29
• 3.3. 화합물 3 (Lipo 14-O-anisoylbikhaconine)...30
• 3.4. 화합물 4 (14-Cinnamoylneoline)...30
• 3.5. 화합물 5 (Mesaconitine)...33
• 3.6. 화합물 6 (Lipomesaconitine)...33
• 3.7. 화합물 7 (Hypaconitine)...33
• 첨부 : Spectra of compounds...35