초록 ▼

본 연구에서는 세계적으로 신기술에 속하는 광섬유와 마이크로어레이 기술을 기반으로 국내의 주요 10종류 식중독 균에 대한 실시간 검출기법을 개발하였다. 우리나라의 식중독균들을 단시간 내에 효율적으로 검출하기 위하여 지노믹스와 immune-proteomics를 활용하여 광섬유와 microarray 코팅용 DNA와 단백질을 개발하고 레이저를 이용한 타깃 검출 기술을 개발, 광섬유와 microarray 기술을 통하여 식품 중에 포함되어 있는 식중독 균을 신속하게 검출할 수 있는 기술을 또한, 센서 반응, 식품 및 농산물 소재 반응분석으로

본 연구에서는 세계적으로 신기술에 속하는 광섬유와 마이크로어레이 기술을 기반으로 국내의 주요 10종류 식중독 균에 대한 실시간 검출기법을 개발하였다. 우리나라의 식중독균들을 단시간 내에 효율적으로 검출하기 위하여 지노믹스와 immune-proteomics를 활용하여 광섬유와 microarray 코팅용 DNA와 단백질을 개발하고 레이저를 이용한 타깃 검출 기술을 개발, 광섬유와 microarray 기술을 통하여 식품 중에 포함되어 있는 식중독 균을 신속하게 검출할 수 있는 기술을 또한, 센서 반응, 식품 및 농산물 소재 반응분석으로 환경스트레스에 의한 특성 변화와 주요 식품의 식중독 세균의 prevalence분석, 개발된 센서 반응의 유효성 평가를 수행하여 식품 및 농산 소재로부터 식중독 세균의 분리 및 전처리 기법을 개발하였다.

Abstract ▼

Using the flourescent labeled antibodies against the specific antigen protein(especially membrane related proteins), The fiber-optic fluoro-immunosensor to detect foodbome pathogens and new fluorescent probe for DNA detection were fabricated and tested. The fabricated system is consisted of the mult

Using the flourescent labeled antibodies against the specific antigen protein(especially membrane related proteins), The fiber-optic fluoro-immunosensor to detect foodbome pathogens and new fluorescent probe for DNA detection were fabricated and tested. The fabricated system is consisted of the multimode optical fiber on which antibodies were immobilized. And a sandwich immunoassay is applied to the fabricated the fiber-optic fluoro-immunosensor. In the 'sandwich' binding format, a primary or 'capture' antibody is immobilized on the core surface of the multimode optical fiber and a secondary or named as 'tracer' antibody was added to the bulk solution. A tracer is that an antibody is labeled FITC. It was tested for characteristics of the detection about Salmonella, Vibrio and Staphylococcus, the nation top three foodbome pathogens, by the fabricated optical system. Most of the antibody-antigen reaction was achieved within 300 seconds and there was non-interference from the fabricated fluoro-immunosensor system by interference tests about other kinds of antigens. The fluorescent probe showed high sensitivity, reliability and stability in the target DNA detection.
Specific 70-mer oligonucleotide probes of major 11 food-borne pathogens were selected by comparative genomics using the Basic Local Alignment Search Tool (BLAST) program between genomic DNA sequences of food-borne pathogen and 70-mer oligonucleotides of 11 food-borne pathogens were constructed. Among constructed probes, 10 probes of each food-borne pathogen were selected and a DNA micro array chip, which is consists of 224 spots, was constructed for the detection of 11 food-borne pathogens. Phylogenetic tree showed high discriminative power that each food-borne pathogen clustered and non-pathogens were discriminated from food borne pathogens. Also, strains in the same food-borne pathogen showed different genetic diversitybased on the micro array results. These results will apply to rapid and convenient method for identification and detection of food-borne pathogens in food industry. In addition, this study demonstrate that genome sequence comparison and DNA microarray has a potential application in epidemiologic and taxonomic studies. Analyses on the prevalence of foodborne pathogens in our foods, their morphological/physiological changes under the food stress, and development of the protocol for improving the detection efficiency by pretreatment concentration of the pathogens were done. E.coli, B.cereus, Staphylococcus aureus, and Salmonella could be the major pathogens in our foods and food stuffs. Their biofilm formed in the foods would be more important inhibitor than the orphological change. To increase the detection efficiency, dilution of food component like acids under 1ppm concentration, pretreatment for pathogen concentration such as filtration, two-step centrifugation, biofilm Triton X100-shaking removal would be needed.

목차 Contents

• 제 1 장 연구개발과제의 개요...77
• 제 2 장 국내외 기술개발 현황...84
• 제 3 장 연구개발수행 내용 및 결과...89
• 제 4 장 목표달성도 및 관련분야에의 기여도...101
• 제 5 장 연구개발결과의 활용계획...104
• 제 7 장 참고문헌...105
• 제 8 장 첨부서류...114