초록 ▼

Human papillomavirus에서 L1 유전자를 분리한 후 baculovirus vector를 이용하여 insect cell에서 L1을 발현하고 분리 정제하여 natural virus와 유사 구조로 assembly된 human papillomavirus-like particle을 수득한다. Mucosal adjuvant로 최근 연구되는 cytokine을 plasmid형태로 construction하였다. Cytokine plasmid를 virus-like particle과 안정한 complex를 형성시키는 방법을 연구하였다.

Human papillomavirus에서 L1 유전자를 분리한 후 baculovirus vector를 이용하여 insect cell에서 L1을 발현하고 분리 정제하여 natural virus와 유사 구조로 assembly된 human papillomavirus-like particle을 수득한다. Mucosal adjuvant로 최근 연구되는 cytokine을 plasmid형태로 construction하였다. Cytokine plasmid를 virus-like particle과 안정한 complex를 형성시키는 방법을 연구하였다. 한편, 점막점착성 고분자를 이용하여 점막 점착시간을 증강시키고 model 항원의 점막 조직 수송율을 높이는 안전성 및 안전성이 검증된 점막 수송체를 개발하여 cytokine plasmid 및 virus-like particle을 점막 점착성 수송체를 이용하여 점막 투여하여 점막 수송체 및 mucosal adjuvant로서 cytokine plasmid의 사용이 virus-like particle vaccine의 점막 면역증가에 미치는 효과를 평가하였다.

Abstract ▼

A major capsid protein L1 gene of human papillomavirus type 16 was amplified by PCR, and expressed in insect cells using a baculovirus vector. Human papillomavirus-like paticle (VLP), self-assembled form of L1 protein, was purified and characterized. As a mucosal adjuvant, cytokine plasmid will be c

A major capsid protein L1 gene of human papillomavirus type 16 was amplified by PCR, and expressed in insect cells using a baculovirus vector. Human papillomavirus-like paticle (VLP), self-assembled form of L1 protein, was purified and characterized. As a mucosal adjuvant, cytokine plasmid will be constructed. Cytokine plasmid was complexed to VLP by various methods. In formulation study, bioadhesive mucosal delivery stems were developed. The mucosal adhesion time of the delivery vehicle, and the uptake of a model antigen by mucosal tissue were tested. Moreover, the safety and the stability of the mucosal delivery system was observed. Developed mucosal delivery system was applied to deliver cytokine plasmid and VLP into mucosal tissue more effectively. The effects of bioadhesive mucosal delivery system, and cytokine plasmid on the mucosal immune response were evaluated.

목차 Contents

• I. 연구계획 요약문...3
• 1. 국문요약문...3
• II. 연구결과 요약문...4
• 1. 국문요약문...4
• 2. 영문요약문...5
• III. 연구내용...6
• 1. 서론...6
• 2. 연구방법 및 이론...8
• 3. 결과 및 고찰...16
• 4. 결론...55
• 5. 인용문헌...56